The Effects of Methamphetamine on the Development  of the Testes in Immature Male Rats

Lotfi, M; Nouri , A; Karimi, A; Pilehvarian, A

Volume 24, Issue 3 (Jun 2016) JSSU 2016, 24(3): 222-231 | Back to browse issues page

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Lotfi M, Nouri A, Karimi A, Pilehvarian A. The Effects of Methamphetamine on the Development of the Testes in Immature Male Rats. JSSU 2016; 24 (3) :222-231
URL: http://jssu.ssu.ac.ir/article-1-3684-en.html

The Effects of Methamphetamine on the Development of the Testes in Immature Male Rats

M Lotfi

, A Nouri ^*

, A Karimi

, A Pilehvarian

Abstract: (6457 Views)

Introduction: Methamphetamine (MAMP) is a central nervous system stimulant that its consumption has increased among youths. Adolescence is considered as a critical period and the reproductive organs are developing is very sensitive so that the. In this study, the effects of methamphetamine on the development of the testes in immature male rats were evaluated.

Methods: 40 immature male Wistar Rats (35-day-old male rats) were divided into four groups: control and the group receiving methamphetamine: (1, 3, 5 mg/kg). The control group received saline and experimental groups received methamphetamine for 10 days (intraperitoneally) and they were allowed to mature. Then, the rats were dissected. Testicularb tissue was isolated and after weighing, they were prepared to be placed in formalin. The results were analyzed by ANOVA and Duncan's test.

Results: Spaces created in the seminiferous tubules showed spermatogenesis disorder. The mean number of cells (spermatogonia, spermatocyte, spermatid, sperm) in the experimental groups decreased significantly than the control group (p<0/001), this reduction was dose dependent. The average weight of the testes to the body weight in the experimental groups showed no significant difference than the control group.

Conclusion: Frequent consumption of methamphetamine, even in low doses through the influence on the pituitary -gonad and the various factors involved in spermatogenesis, can disrupt the sexual maturation, which may cause reduced fertility in males.

Keywords: Methamphetamine, Spermatogenesis, Testis, Rats

Full-Text [PDF 892 kb] (1988 Downloads)

Type of Study: Original article | Subject: Biology
Received: 2016/04/14 | Accepted: 2016/06/5 | Published: 2016/09/11

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