20 2228-5741 Shahid Sadoughi University of Medical Sciences 4016 Medical Physics Investigation of Reduction of the Uncertainty of Monte Carlo Dose Calculations in Oncor® Clinical Linear Accelerator Simulation Using the DBS Variance Reduction Technique in Monte Carlo Code BEAMnrc Asadi Amin b Nickfarjam Abolfazl c Jabari Keyvan d b Shahid Sadoughi University of Medical Sciences and Health Services, Yazd c Shahid Sadoughi University of Medical Sciences and Health Services, Yazd d Faculty of Medicine, Isfahan University of Medical Sciences 1 10 2017 25 7 512 525 19 12 2016 09 07 2017 Purpose: To study the benefits of Directional Bremsstrahlung Splitting (DBS) dose variance reduction technique in BEAMnrc Monte Carlo (MC) code for Oncor® linac at 6MV and 18MV energies. Materials and Method: A MC model of Oncor® linac was built using BEAMnrc MC Code and verified by the measured data for 6MV and 18MV energies of various field sizes. Then Oncor® machine was modeled running DBS technique, and the efficiency of total fluence and spatial fluence for electron and photon, the efficiency of dose variance reduction of MC calculations for PDD on the central beam axis and lateral dose profile across the nominal field was measured and compared. Result: With applying DBS technique, the total fluence of electron and photon increased in turn 626.8 (6MV) and 983.4 (6MV), and 285.6 (18MV) and 737.8 (18MV), the spatial fluence of electron and photon improved in turn 308.6±1.35% (6MV) and 480.38±0.43% (6MV), and 153±0.9% (18MV) and 462.6±0.27% (18MV). Moreover, by running DBS technique, the efficiency of dose variance reduction for PDD MC dose calculations before maximum dose point and after dose maximum point enhanced 187.8±0.68% (6MV) and 184.6±0.65% (6MV), 156±0.43% (18MV) and 153±0.37% (18MV), respectively, and the efficiency of MC calculations for lateral dose profile remarkably on the central beam axis and across the treatment field raised in turn 197±0.66% (6MV) and 214.6±0.73% (6MV), 175±0.36% (18MV) and 181.4±0.45% (18MV). Conclusion: Applying dose variance reduction technique of DBS for modeling Oncor® linac with using BEAMnrc MC Code surprisingly improved the fluence of electron and photon, and it therefore enhanced the efficiency of dose variance reduction for MC calculations. As a result, running DBS in different kinds of MC simulation Codes might be beneficent in reducing the uncertainty of MC calculations. 
3717 Biochemistry Effect of resveratrol the expression of some genes involved epigenetic in breast cancer cell lines (MCF-7, MDA-MB-453) Ahmadirad Hadis e Hajizadeh Mohammad Reza Hajizadeh f Mahmoodi Mehdi g Mirzaee Mohamadreza h Shahrbabaki Fahime Mohammadain i Soltaninejad Mostafa j e University of Medical Sciences, Rafsanjan, Iran f Molecular Medicine Research Center, Medical School, Rafsanjan University of Medical Sciences, Rafsanjan, Iran g Rafsanjan University of Medical Sciences, Rafsanjan, Iran h Medical School, Rafsanjan University of Medical Sciences, Rafsanjan, Iran i University of Medical Sciences, Rafsanjan, Iran j Rafsanjan University of Medical Sciences, Rafsanjan, Iran 1 10 2017 25 7 526 536 09 05 2016 11 03 2017 Background: Breast cancer is one of the most common cancers among women.  Incorrect pattern of gene expression involved in epigenetic  Including APOBEC3B, DNMT-1 and TET_1 can develop breast cancer.   resveratrol is a natural flavonoids that antioxidant and anti-cancer properties have been reported in other studies. To investigate the mechanism of effect of resveratrol, this study examined  effect of  resveratrol on the expression of genes referred to the two classes of  breast cancer cell lines Materials and methods: cell lines, including MCF-7 and MDA-MB-453 in separate boxes including the control group and treated groups with two doses of two doses of 25 and 100 micro molar for 24 hours of resveratrol for were cultured. RNA was extracted from the cells and then converted to cDNA. realtime PCR were used for APOBEC3B and DNMT and TET_1expression. Results: The results showed that resveratrol decrease in gene expression APOBEC3B and  DNMT-1 And increased expression of  TET_1 in both of cell lines.  . Discussion: due to satisfactory effects of resveratrol has on breast cancer cells, probably these effects  through  epigenetic mechanisms applied.  However, the final decision needs to be further investigation. 4059 Immunology Evaluation of three different administration routes (IM, SC and IN) on humoral immune responses against Mycobacterium Tuberculosis ESAT-6/CFP-10 fusion protein Namvarpour Mojdeh k Mansouri Reza l Tebianian Majid m k Shahid sadoughi university of medical sciences, Yazd l Shahid sadoughi university of medical sciences, Yazd m Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran 1 10 2017 25 7 537 546 08 01 2017 20 05 2017 Introduction: Tuberculosis (TB) has been considered as a main health problems of the present century. Unfortunately, it has been reported different protective responses against BCG vaccine, as only available vaccine against TB .The search for a new and improved vaccine against tuberculosis is currently a very active field of research. In this study, we have evaluated the relative effects of intranasal (i.n), subcutaneous (s.c) and intramuscular (i.m) routes of immunization on the induction of humoral immune responses against TB recombinant protein. Methods: The recombinant ESAT-6/CFP-10 antigens has been assessed and confirmed preliminary, for this study. The Balb/C mice were immunized in 3 different routes, three times at 2-week intervals with recombinant protein, formulated with or without adjuvants (MF-59 for the i.m and s.c routes and CTB for i.n). Blood was collected from retro-orbital sinus 10 days after each immunization and after separation of sera, they were evaluated for specific antibodies against antigen by an indirect ELISA method, which had set up during the research. Results: Mice vaccinated against recombinant proteins had high level of specific antibodies compared to the controls. Our results showed that although the i.m and s.c injections also induce immune responses, but the antibody titer in i.n rout had graduately elevated to reach in acceptable levels. Conclusion: The results could be used for formulation of mucosal vaccines against tuberculosis in future studies. 4197 Biochemistry The level of gene expression of Bax and Bcl-2 and the activity of caspase 3 in the liver tissues of normal, type 1 and type 2 diabetic rats before and after treatment with aqueous extract of garlic Karimi Mohammad Naser Abbasalipourkabir Roghayeh Arab Sadeghabadi Zahra Ziamajidi Nasrin 1 10 2017 25 7 547 555 11 05 2017 03 06 2017 Introduction: One of the complications of diabetes mellitus is induction of apoptosis in liver tissues. Activation of caspase 3 caused apoptosis in the cells. Garlic is a most common plant in diet that its effect on apoptosis was not determined. In this study the effects of this extract on Bax and Bcl-2 gene expression and the activity of caspase 3, were determined in type 1 and 2 of diabetes in rats. Methods: Wistar rats divided into six groups included normal control rats, diabetes type 1 (D1), diabetes type 1 treated with garlic extract (D1G), diabetes type 2 (D2), diabetes type 2 treated with garlic extract (D2G) and garlic control (G). At the end of treatment time, liver tissues were removed quickly and stored in -70 °C. For evaluation of gene expression, RNA extraction, cDNA synthesis and Real time PCR were done. The activity of caspase 3 was determined with flourometric method. Results: The level of gene expression of Bax and Bcl-2, did not change significantly in different groups. The activity of caspase 3 in D1 rats, increased significantly compared to the control rats(p=0.003). The activity of this enzyme was high in D1G compared to control rats yet (p=0.021). In D2, D2G and G groups, caspase activity was not changed in comparison with the control rats Conclusion: Diabetes mellitus type1 caused apoptosis in rat livers, but garlic extract cannot ameliorate it. 4122 Obstetrics Evaluation of severity of preeclampsia with mother’s serum high sensitive C-reactive protein (Hs-CRP) Samiee Rad Fatemeh Jahani Hashemi Hassan Sofiabadi Mohammad Bahrami Hamidreza 1 10 2017 25 7 556 563 20 02 2017 29 07 2017 Introduction: Preeclampsia is one of the most common complications in pregnancy and a main cause of death in the perinatal period. The early diagnosis of severe disease form is important. The aim of this study was to evaluate the relationship between the serum high sensitive C - reactive protein (hs-CRP) and severity of preeclampsia. Methods: This cross-sectional study conducted between 2013 October -2014 February in third trimester pregnant women that had been admitted to Qazvin Kosar Hospital they were divided into three groups as: the first included 50 healthy pregnant women (control), the second included 45 women with mild preeclampsia and the third group included 42 women with severe pre-eclampsia. Blood pressure was measured after at least 10 minutes resting in a sitting position. Blood samples were taken six hours after admission and the hs-CRP measured by immunoturbidometric method using bionic kit. Data were analyzed using ANOVA, x2 and Kruskal-Wallis tests. Results: The average serum levels of hs-CRP were 2.4 in the control, 8.7 in the mild and 32.6 in the severe preeclampsia groups and this difference were significant in all the three groups. Conclusion: According to the results, it seems that the serum levels of hs-CRP can be used in the prediction and diagnosis of the severity of preeclampsia. 3765 Microbiology Comparison of Genomic Polymorphisms and Genetic Relation of Clinical Salmonella Enterica Serovar Typhimurium Isolates in Kerman Province by ERIC- PCR and Box-PCR Methods Geranmayeh Arghavan Hoseini Farzaneh Rafiei Tabatabaei Robab Department of Microbiology, College of Bioscience, Islamic Azad University Tehran North Branch, Tehran, Iran. Department of Microbiology, College of Bioscience, Islamic Azad University Tehran North Branch, Tehran, Iran. Department of Microbiology, College of Bioscience, Islamic Azad University Tehran North Branch, Tehran, Iran. 1 10 2017 25 7 564 571 10 06 2016 24 12 2016 Introduction: Salmonella is one of the most important causes of gastroenteritis in humans. Salmonella enterica Serovar Typhimurium has many hosts in addition to humans, and its prevalence in the community is high. The aim of the study was comparing the genetic diversity of Salmonella enterica serovar Typhimurium isolated from human fecal samples by both of ERIC-PCR and BOX-PCR method. Methods: In this cross-sectional study, 60 Salmonella enterica serovar Typhimurium were obtained from the human fecal samples. These strains were identified by standard microbiological and biochemical tests. Then, ERIC-PCR and BOX-PCR were carried out for determination of molecular relatedness of Salmonella enterica serovar Typhimurium using specific primers. Results: The results showed that all 60 Salmonella enterica serovar Typhimurium were separable using ERIC 1, ERIC 2 and BOXAIR. In electrophoresis, 2-11 bands with 20-3200bp for ERIC-PCR and 2-10 bands with 200-1500bp for BOX-PCR were observed. Therefore, 13 different clusters (C1-C13) in ERIC-PCR and 21 different clusters (C1-C21) in BOX-PCR were identified. Conclusion: The results showed that Salmonella enterica serovar Typhimurium strains were non-homolog. Therefore, ERIC-PCR and BOX-PCR methods are appropriate methods for molecular typing of Salmonella strains and determine the original infection source for the epidemiological survey as well as infection prevention program. 4135 Biochemistry Effects of Exposure to WI-FI Signals (2.45 GHz) on Serum Oxidative Stress and Single Strand DNA Breaks in Peripheral Blood Lymphocytes of Mice borhan Fatemeh Zavvar-reza Javad Pandeh Masoud Fathi Saedeh ‏ Department of Clinical biochemistry, Shahid Saddughi University of Medical Sciences, Yazd, Iran Department of Clinical biochemistry, Shahid Saddughi University of Medical Sciences, Yazd, Iran Department of Clinical biochemistry, Shahid Saddughi University of Medical Sciences, Yazd, Iran 1 10 2017 25 7 572 584 05 03 2017 09 07 2017 Introduction: Use of wireless devices have been increasing during the last three decades in the world. Health risks caused by exposure to these appliances has become a public concern. The purpose of this study was to investigate the effects of exposure to Wi-Fi on DNA breaks and oxidative stress parameters. Methods:16 male mice divided in 2 groups; experimental (exposed) (n=8) and the control groups (n=8). While the control group was kept not exposed to the signals the experimental group was exposed to 2.45 GHz Wi-Fi signal GHz, for 8h/21 day; Single strand DNA breaks in lymphocytes were determined by using the Alkaline Comet Assay (before and after the exposure period). Oxidative stress parameters, including, catalase activity, PON1 activity, and TAC were measured (before and after the exposure period). Results: In the exposed group, it was observed an increase in single-stranded DNA break; decrease in PON1 enzyme activity also increase the catalase activity compared to before exposure to wi-fi,  but TAC was not significantly different. While in the control group, none of the indicators measured at the end of the study there was no significant difference compared to 21 days ago. Conclusion: The findings of the present study show that Wi-Fi exposure can increase oxidative stress and the strand DNA breaks 4096 Physiology Comparison of sperm PLCζ protein and DNA damage between fertile and infertile men tavalaee Marziyeh Arbabian Maryam Nasr- Esfahani Mohammad Hossein Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran 1 10 2017 25 7 585 594 04 02 2017 20 05 2017 Introduction: Sperm-specific phospholipase C zeta (PLCζ) protein is expressed during spermatogenesis and plays a main role during oocyte activation. In this study, the expression of sperm PLCζ protein and DNA damage in fertile and infertile men was assessed. Methods: In this case-control study, semen samples were collected from 15 fertile and 10 infertile men candidated for intra-cytoplasmic sperm injection (ICSI). Sperm parameters, the expression of PLCζ protein, and DNA damage were assessed by the World Health Organization (2010) protocol, Western Blot, and TUNEL assay, respectively. Results: The quality of sperm parameters were significantly lower in the infertile men compared with the fertile men. In addition, the expression of PLCζ protein was significantly lower, and percentage of sperm DNA damage were significantly higher in infertile men than fertile men.  Conclusion: Our results clearly showed that low or absence expression of PLCζ and sperm DNA damage could be considered as factors involved in failed fertilization in these infertile men. Therefore, the evaluation of some tests such as PLCζ protein and chromatin tests to assess fertilization potential of a semen sample for infertile men are recommended.