fa بررسی اثر عصاره اسپیرولینا پلاتنسیس بر بقای سلولی و تغییر بیان ژن آپوپتوزی BAD در سلول‌های آدنوکارسینومای معده انسان AGS عمومى General پژوهشي Original article <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در این مطالعه اثر عصاره اسپیرولینا پلاتنسیس با خواص ضد التهابی و پیشگیری از سرطان و تقویت سیستم ایمنی بر بقای سلولی و تغییر بیان ژن آپوپتوزی </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">BAD</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در سلول‌های آدنوکارسینومای معده انسان رده </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">AGS</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> مورد بررسی قرار گرفت.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در پژوهش تجربی حاضر، با کشت رده سلولی </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">AGS</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در محیط کشت </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">DMEM-F12</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> حاوی 10% سرم جنین گاوی و تیمار سلول‌ها در غلظت‌های 10، 20 و 40 میکرو</span></span><span dir="LTR" style="font-size:10.0pt">‌</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">گرم/ میلی</span></span><span dir="LTR" style="font-size:10.0pt">‌</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">لیتر از عصاره جلبک اسپیرولینا پلاتنسیس و انکوباسیون 24 و 48 ساعته، سمیت سلولی با روش رنگ‌سنجی </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MTT</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> طبق دستورالعمل کیت در هر دو زمان انکوباسیون مورد بررسی قرار گرفت. سلول</span></span><span dir="LTR" style="font-size:10.0pt">‌</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های رده </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">AGS</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> سپس با غلظتی از عصاره که سبب کاهش 50 درصدی بقای سلولی گردید (40 میکرو</span></span><span dir="LTR" style="font-size:10.0pt">‌</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">گرم/</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">میلی</span></span><span dir="LTR" style="font-size:10.0pt">‌</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">لیتر) برای بررسی بیان ژن</span></span><span dir="LTR" style="font-size:10.0pt">‌</span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">BAD</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> ظرف مدت 48 ساعت انکوبه شدند. سپس میزان بیان ژن</span></span><span dir="LTR" style="font-size:10.0pt">‌</span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">BAD</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با آنالیز </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RT-Real Time- PCR</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> مورد بررسی قرار گرفت. آنالیز آماری با استفاده از نرم‌افزار </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">SPSS version 16</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و آزمون من ویتنی انجام شد.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> نتایج این پژوهش نشان داد که بقای سلولی سلول</span></span><span dir="LTR" style="font-size:10.0pt">‌</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های رده </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">AGS</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> آدنوکارسینومای معده، در مقایسه با گروه کنترل در دوز 20 (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P<0.001</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) و 40 (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P<0.0001</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) میکرو</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">گرم/ میلی</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">لیتر در زمان انکوباسیون 48 ساعت به طور معنی</span></span><span dir="LTR" style="font-size:10.0pt">‌</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">داری کاهش می</span></span><span dir="LTR" style="font-size:10.0pt">‌</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">یابد. به‌علاوه تیمار سلول</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&not;</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های رده </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">AGS</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">با دوز 40 میکرو</span></span><span dir="LTR" style="font-size:10.0pt">‌</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">گرم/ میلی</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">لیتر عصاره جلبک اسپیرولینا پلاتنسیس و انکوباسیون آن برای مدت 48 ساعت، افزایش معنی</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">دار بیان ژن (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">BAD (P<0.001</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> را نشان داد. </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجه‌گیری:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> نتایج بیانگر آن هستند که عصاره جلبک اسپیرولینا پلاتنسیس قادر است با القاء بیان ژن </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">BAD</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و جلوگیری از تکثیر، از بقای سلولی سلول</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های آدنوکارسینومای معده بکاهد.</span></span></span></span></span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Introduction:</span></b> <span new="" roman="" style="font-family:" times="">In this study, the influence of Spirulina platensis extract with anti-inflammatory, cancer prevention and immune system strengthening properties on cell viability and changes in BAD apoptotic gene expression in human gastric adenocarcinoma cells of&nbsp; the AGS line was investigated.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><span new="" roman="" style="font-family:" times="">Conclusion: The results indicate that Spirulina platensis algae extract is able to reduce the cell survival of gastric adenocarcinoma cells by inducing BAD gene expression and preventing proliferation.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b><span new="" roman="" style="font-family:" times=""> In the present experimental study, by culturing the AGS cell line in DMEM-12 medium with 10% fetal bovine serum and treating the cells at concentrations of </span><span new="" roman="" style="font-family:" times="">10, 20 and 40 &mu;g/ml of Spirulina platensis algae extract and incubating for 24 and 48 hours, cytotoxicity was examined using the MTT colorimetric method according to the kit instructions at both incubation times. </span><span new="" roman="" style="font-family:" times="">The AGS line cells were then incubated &nbsp;with a concentration of Spirulina platensis extract at IC50 point (40 &micro;g /mL) for 48 hours to assess the expression level of the BAD gene via real-time RT-PCR. Statistical analysis was accomplished using Mann-Whitney test using SPSS 16 software.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><b> </b><span new="" roman="" style="font-family:" times="">The results of this study indicated that the cell viability of AGS gastric Adenocarcinoma cell line significantly decreased at concentration of 20 (P<0.001) and 40 (P<0.0001) &micro;g/mL compared to the control group. In addition, AGS cell line treatment with Spirulina platensis extract at a dose of 40 &micro;g/mL and 48 incubation time, showed a significant increased expression of BAD gene (P<0.001).</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><b> </b><span new="" roman="" style="font-family:" times="">The results indicate that Spirulina platensis extract can reduce the viability of gastric adenocarcinoma cells by inducing expression of BAD gene that preventing proliferation.&nbsp; </span><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br> &nbsp;</div> آدنوکارسینومای معده, بیان ژن, BAD, اسپیرولینا پلاتنسیس Gastric Adenocarcinoma, Gene Expression, BAD, Spirulina platnissis. 9639 9649 http://jssu.ssu.ac.ir/browse.php?a_code=A-10-3568-3&slc_lang=fa&sid=1 Seyed Jahanbakhsh Daniyali سید جهانبخش دانیالی javandanyali@gmail.com 0009-0003-4853-6517 No Department of Biology, Islamic Azad University, Shahrekord, Iran گروه زیست‌شناسی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران. Leila Rouhi لیلا روحی lrouhi59@gmail.com 0000-0003-1545-5158 Yes Department of Biology, Islamic Azad University, Shahrekord, Iran گروه زیست‌شناسی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران.