fa تاثیر شش هفته پیش آماده‌سازی با تمرین تداومی و تناوبی پرشدت بر مقادیر miR-132، miR-134، وحجم سکته مغزی در رت‌های صحرایی نر : مدل سکته مغزی فیزیولوژی ورزش Exercise Physiology پژوهشي Original article <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">متخصصان علوم ورزشی هنوز در مورد یک دستورالعمل ورزشی جامع جهت پیشگیری و درمان سکته مغزی به نتیجه قطعی نرسیده</span></span>‌<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">اند. لذا هدف از تحقیق حاضر تاثیر شش هفته پیش آماده&shy;سازی با تمرین تداومی و تناوبی پرشدت بر مقادیر</span></span><span dir="LTR" new="" roman="" style="font-family:" times=""> miR-132 </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">miR-134</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، حجم سکته مغزی و نقص نورولوژیک در رت‌های صحرایی نر: مدل سکته مغزی بود.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در این پژوهش تجربی، 24 سر رت نر (هشت هفته ای از نژاد ویستار) به چهار گروه (6 سر در هر گروه) شم، </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HIIT</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MICT</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و کنترل تقسیم شدند. برنامه تمرین گروه </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HIIT</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> (6 ست دو </span></span><span lang="FA" style="font-size:12.0pt">‌</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">دقیقه ای با 85-90% </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">VO2max</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در مرحله فعالیت و 5 ست 2 دقیقه</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ای با 65% </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">VO2max</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در مرحله استراحت) و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MICT</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> (با 65% </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">VO2max</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) پنج جلسه در هفته، به مدت شش هفته اجرا شد. در پایان بافت‌های هیپوکمپ، کورتکس و استریاتوم مغز جهت سنجش بیان </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">miR-132</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">miR-134</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با استفاده از روش </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Real Time PCR</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> اندازه‌گیری گردید. تحلیل داده</span></span><span lang="FA" style="font-size:12.0pt">&not;</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ها با استفاده از آزمون آنالیز واریانس یکراهه و آزمون تعقیبی بونفرونی (0/05<</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) به کمک نرم</span></span><span lang="FA" style="font-size:12.0pt">&not;</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">افزار </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">SPSS version 16</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> انجام شد.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">هر دو مدل تمرین پیش آماده‌سازی باعث افزایش معنی‌دار بیان </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">miR-132</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در ناحیه هیپوکمپ نسبت به گروه کنترل شدند (0/05<</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">)؛ اما در ناحیه کورتکس </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MICT</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و در ناحیه استریاتوم، </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HIIT</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> این اثر را داشت</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> HIIT</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> (0/05&nbsp;</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">(p<. </span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&nbsp;موجب افزایش معنی‌دار بیان </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">miR-134</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در ناحیه کورتکس شد و در ناحیه استریاتوم کاهش معنی‌دار نشان داد (0/05<</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">). در متغیرهای حجم سکته مغزی و نقص نورولوژیک بین هر دو گروه تمرینی </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HIIT</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MICT</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با گروه کنترل تفاوت معنی‌دار وجود داشت (0/05<</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">).</span></span> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجه‌گیری:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">انجام فعالیت</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های ورزشی در طول عمر می تواند تاثیر مثبت بر بخش</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های مختلف مغز بگذارد و پس از وقوع سکته مغزی به بیمار کمک کند. لذا انجام فعالیت ورزشی منظم می‌تواند عوارض حاد و مزمن سکته مغزی را به حداقل برساند.</span></span></span></span></span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Introduction:</span></b> <span new="" roman="" style="font-family:" times="">Sports science specialists have not yet reached a definitive or universal exercise guideline for stroke prevention and treatment. Therefore, the aim of the present study was to investigate the effect of six weeks of continuous and high-intensity interval training on miR-132 and miR-134 levels, stroke volume, and neurological deficits in male rats, a stroke model.&nbsp; </span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b><span new="" roman="" style="font-family:" times=""> Twenty-four eight-week-old male Wistar rats were divided into four groups (n=6 per group): sham, HIIT, MICT, and control. The HIIT (2&times;6 min, 85-90% VO2 max activity and 2&times;5 min, 65% VO2 max rest) and MICT (65% VO2 max) were performed five sessions per week for four weeks. At the end of the intervention, hippocampal, cortical, and striatum tissues were measured to measure the expression levels of miR-132 and miR-134 using Real-Time PCR. Data analysis was performed using one-way ANOVA followed by the Bonferroni post hoc test (p<0.05) with SPSS version 16.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><b> </b><span new="" roman="" style="font-family:" times="">Both training modalities significantly increased miR-132 expression in the hippocampus (p<0.05); however, HIIT also produced this effect in the cortex and the striatum compared with MICT (p<0.05). HIIT significantly increased miR-134 expression in the cortex and significantly decreased its expression in the striatum (p<0.05). In addition, there was a significant difference in the variables of stroke volume and neurological deficit between both the HIIT and MICT training groups and the control group (p<0.05).</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><b> </b><span new="" roman="" style="font-family:" times="">Performing exercise activities throughout life can have a positive effect on different parts of the brain and help the patient after a stroke. Therefore, it can be concluded that regular exercise may help to minimize both the acute and chronic complications of stroke.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> &nbsp;</div> سکته مغزی, miR-132 , miR-134, فعالیت ورزشی Stroke, microRNA-132, microRNA-134, Exercise. 9922 9936 http://jssu.ssu.ac.ir/browse.php?a_code=A-10-5490-1&slc_lang=fa&sid=1 Mohammad Baqer Azizi محمدباقر عزیزی azizi8998@gmail.com 0000-0009-1158-0846 No Department of Sport Sciences, Faculty of Educational Sciences and Psychology, Shiraz University, Shiraz, Iran. گروه علوم ورزشی، دانشکده علوم تربیتی و روانشناسی، دانشگاه شیراز، شیراز، ایران Rasoul Rezaei رسول رضایی rasoul.rezai1364@gmail.com 0000-0001-5136-3694 No Department of Sport Sciences, Faculty of Educational Sciences and Psychology, Shiraz University, Shiraz, Iran. گروه علوم ورزشی، دانشکده علوم تربیتی و روانشناسی، دانشگاه شیراز، شیراز، ایران Mohsen Salesi محسن ثالثی mhsnsls@gmail.com 0000-0003-2969-9102 Yes Department of Sport Sciences, Faculty of Educational Sciences and Psychology, Shiraz University, Shiraz, Iran. گروه علوم ورزشی، دانشکده علوم تربیتی و روانشناسی، دانشگاه شیراز، شیراز، ایران Javad Nemati جواد نعمتی nemati_phy@yahoo.com 0000-0002-6925-1727 No Department of Sport Sciences, Faculty of Educational Sciences and Psychology, Shiraz University, Shiraz, Iran. گروه علوم ورزشی، دانشکده علوم تربیتی و روانشناسی، دانشگاه شیراز، شیراز، ایران