fa بررسی اثر عصاره اسپیرولینا پلاتنسیس بر میزان بیان ژن آپوپتوزی Drp1 و تنظیم مرگ سلولی برنامه‌ریزی شده در رده سلولی آدنوکارسینومای معده انسان (AGS) عمومى General پژوهشي Original article <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">در بین سرطان&shy;ها، آدنوکارسینومای معده یکی از شایع&shy;ترین سرطان&shy;ها در سراسر جهان می&shy;باشد. این نوع بدخیمی به شدت تهاجمی بوده و مبتلایان پاسخ به درمان ضعیفی دارند. </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">از این‌رو در مطالعه حاضر اثر عصاره اسپیرولینا پلاتنسیس بر میزان بیان ژن آپوپتوزی </span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Drp1</span></span></i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و تنظیم مرگ سلولی برنامه‌ریزی شده در رده سلولی آدنوکارسینومای معده انسان (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">AGS</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) مورد بررسی قرار گرفت.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در این مطالعه سلول</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&shy;</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های سرطانی معده رده </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">AGS</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در محیط کشت </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">DMEM-F12</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با 10 درصد سرم جنین گاوی کشت داده شدند. با تیمار سلول</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&shy;</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ها در غلظت 40 میکرو</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">رم/ میلی</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">لیتر از عصاره جلبک اسپیرولینا و انکوبه شدن در زمان</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> 48 ساعت، میزان القا آپوپتوز به‌وسیله دستگاه فلوسیتومتری با کیت آنکسین-پروپیدیوم یدید (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Annexin-PI</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) طبق دستورالعمل کیت مورد بررسی قرار گرفت. سلول</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&shy;</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های رده </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">AGS</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> نیز با غلظت 40 میکرو</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">گرم/ میلی</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">لیتر از عصاره جلبک اسپیرولینا برای بررسی بیان ژن </span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Drp1</span></span></i><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> ظرف مدت 48 ساعت انکوبه شدند. سپس میزان بیان ژن از طریق آنالیز </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RT-Real Time- PCR</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> مورد بررسی قرار گرفت.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> نتیجه تست انکسین نشان می</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">دهد که سلول</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های رده </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">AGS</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در تیمار با غلظت 40 میکرو</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">گرم/ میلی</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">لیتر از عصاره جلبک اسپیرولینا دچار آپوپتوز می</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">شوند. پرسش‌نامه تیمار با عصاره جلبک اسپیرولینا سبب افزایش معنی‌دار بیان ژن</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span> <i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Drp1</span></span></i><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در گروه</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> آزمایشی نسبت به گروه کنترل گردید.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجه‌گیری:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> نتایج بیان می</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">کنند که اسید سیتریک قادر است از طریق القاء مسیر آپوپتوزی توان زیستی سلول</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های آدنوکارسینومای معده را کاهش دهد. لذا به ‌نظر می</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&shy;</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سد اسید سیتریک </span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بتواند به‌ عنوان یک ماده ضد سرطان در راستای درمان سرطان معده به‌ کار رود.</span></span></span></span></span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Introduction:</span></b> <span new="" roman="" style="font-family:" times="">Gastric adenocarcinoma ranks among the most prevalent cancers globally. This aggressive malignancy often leads to poor treatment outcomes for the patients. This study investigated the effect of &nbsp;Spirulina platensis extract on the expression level of apoptosis-related gene <i>Drp1</i> and its role in regulating programmed cell death in the human gastric adenocarcinoma cell line AGS</span><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b><span new="" roman="" style="font-family:" times=""> AGS gastric adenocarcinoma cells were cultured in DMEM-F12 medium supplemented with 10% bovine serum. The cells were treated in 40 &micro;g/mL of Spirulina extract and incubated for 48 hours. Apoptosis was analyzed using flow- cytometry with an Annexin V-FITC/PI kit, following the manufacturer&#39;s protocol. Moreover, AGS cells were incubated with the same concentrations of Spirulina extract for 48 hours to evaluate <i>Drp1</i> gene expression, which was analyzed using Real Time PCR.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><b> </b><span new="" roman="" style="font-family:" times="">The Annexin V test results showed that treatment with 40 &micro;g/mL of Spirulina extract induced apoptosis in AGS cells. Furthermore, this treatment significantly increased the expression of <i>Drp1</i> gene in the experimental group compared to the control group.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><b> </b><span new="" roman="" style="font-family:" times="">The results indicate that Spirulina extract can enhance the bioavailability of gastric adenocarcinoma cells by inducing apoptosis pathway. Consequently, Spirulina extract seems to be used as an anticancer agent for treating gastric adenocarcinoma</span><span new="" roman="" style="font-family:" times="">. </span></span></span></span></span><br> &nbsp;</div> آدنوکارسینومای معده, جلبک اسپیرولینا پلاتنسیس, ژنDrp1, آپوپتوز Gastric Adenocarcinoma, Spirulina platensis algae, Drp1 Gene, Apoptosis 8974 8984 http://jssu.ssu.ac.ir/browse.php?a_code=A-10-3568-2&slc_lang=fa&sid=1 Farkhondeh Mohammadi فرخنده محمدی farkhondehmhd99@gmail.com 0009-0006-9854-7726 No Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran گروه زیست شناسی، دانشکده علوم پایه، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران. Leila Rouhi لیلا روحی lrouhi59@gmail.com 0000-0003-1545-5158 Yes Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran گروه زیست شناسی، دانشکده علوم پایه، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران.