The Journal of Shahid Sadoughi University of Medical Sciences
مجله علمي پژوهشي دانشگاه علوم پزشكي شهید صدوقی يزد
JSSU
Medical Sciences
http://jssu.ssu.ac.ir
20
journal20
2228-5741
2228-5733
fa
jalali
1404
1
1
gregorian
2025
4
1
33
1
online
1
fulltext
fa
تاثیر تمرین ورزشی شنا و مکمل آنتیاکسیدانی پیشرفته (MitoQ) بر مسیر سیگنالینگ TWEAK/TRAF2/ERK1/Ap-1 در عضله اسکلتی موش های نر دارای آسیب نخاعی
Effect of Swimming Exercise Training and Advanced Antioxidant Supplementation on the TWEAK/TRAF2/ERK1/Ap-1 Signaling Pathway in Skeletal Muscle of Male Mice with Spinal Cord Injury
فیزیولوژی ورزش
Exercise Physiology
پژوهشي
Original article
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">آسیب نخاعی یک بیماری تخریب کننده عصبی است که منجر به کاهش عملکرد حرکتی و آتروفی عضله اسکلتی میشود.</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> هدف </span></span></span><strong><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">از مطالعه حاضر، بررسی اثرات یک دوره تمرین استقامتی و استفاده از مکمل مایتوکیو بر مسیر سیگنالینگ آتروفی در عضله اسکلتی موشهای نر دچار آسیب نخاعی است.</span></span></span></strong><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در این مطالعه، تعداد ۴۰ سر موش صحرایی نر به صورت تصادفی در ۵ گروه (۸</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">n=</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) ۱) شم، 2) آسیب نخاعی (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Spinal</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Cord Injury: SCI</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">)، ۳) آسیب نخاعی و مکمل </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MitoQ</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> (۲۵۰ میکرومولار)، ۴) آسیب نخاعی و ورزش شنا (4 هفته، 5 روز در هفته و هر جلسه ۶ مرحله ۵ دقیقه ای) و 5) آسیب نخاعی و شنا و مکمل </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MitoQ</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> تقسیم شدند. برای ارزیابی متغیرها از روش </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Real time RT-PCR</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> استفاده گردید. آنالیز آماری دادهها با استفاده از آزمون </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">one-way ANOVA</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و آزمون تعقیبی توکی در سطح معنیداری 0/05</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p <</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و با نرمافزار</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">version 16</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">SPSS</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> انجام شد.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> چهار هفته تمرین شنا بهطور معنیداری میزان بیان ژن</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">TWEAK </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">،</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">TRAF2</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">AP-1</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> را در گروههای تمرین در مقایسه با گروه آسیب نخاعی کاهش داد (0/05</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p< </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) . همچنین، تمرین استقامتی با مصرف مکمل </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MitoQ</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> بهطور موثرتری مقادیر</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">mRNA</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> شاخص های</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> TWEAK </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">(0/001</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p =</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">)، </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">TRAF2</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> (0/001</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p =</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">AP-1</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> (0/001</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p= </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) را در عضله اسکلتی رتهای نر دچار آسیب نخاعی در مقایسه با گروه آسیب کاهش داد.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجهگیری:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> تعامل 4 هفته تمرین شنا و مصرف مکمل مایتوکیو میتواند بهطور موثری از بیان ژنهای درگیر در فرآیند آتروفی عضله اسکلتی در موشهای دارای آسیب نخاعی جلوگیری کند و موجب بهبود عملکرد حرکتی در موشهای دچار آسیب نخاعی شود.</span></span></span></span></span></span></span><br>
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<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:16.0pt"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Introduction:</span></b> <span new="" roman="" style="font-family:" times="">Spinal cord injury is a neurodegenerative condition that leads to reduced motor function and skeletal muscle atrophy. The aim of the present study was to investigate the effects of a period of endurance training and the administration of MitoQ supplementation on the atrophy signaling pathway in the skeletal muscle of male rats with spinal cord injury.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:16.0pt"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b><span new="" roman="" style="font-family:" times=""> In this study, a total of 40 male rats were randomly divided into 5 groups (n=8 each): 1) sham, 2) spinal cord injury (SCI), 3) spinal cord injury and MitoQ supplementation (250 μM), 4) spinal cord injury and swimming exercise (4 weeks, 5 days a week, 6 sessions of 5 minutes), and 5) spinal cord injury and swimming and MitoQ supplementation. Real-time RT-PCR was utilized to evaluate the variables. For statistical analysis, data were analyzed one-way ANOVA along with Tukey's post hoc test, applying a significance level of p < 0.05, utilizing and with SPSS software 16.</span><b><span style="font-family:"Times New Roman",serif"></span></b></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:16.0pt"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><span new="" roman="" style="font-family:" times=""> After four weeks of swimming training, there was a marked decrease in the expression of TWEAK, TRAF2, and AP-1 genes in the training groups when compared to the spinal cord injury group (p < 0.05). Furthermore, endurance training combined with MitoQ supplementation led to a more significant reduction in the mRNA levels of TWEAK (p = 0.001), TRAF2 (p = 0.001), and AP-1 (p = 0.001) within the skeletal muscle of male rats suffering from spinal cord injury, in contrast to the injury group.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:16.0pt"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><span new="" roman="" style="font-family:" times=""> The interaction of 4 weeks of swimming training and MitoQ supplementation can effectively prevent the expression of genes involved in the process of skeletal muscle atrophy in mice with spinal cord injury and improve motor function in mice with spinal cord injury.</span></span></span></span></span><br>
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تمرین ورزشی, MitoQ, آتروفی, آسیب نخاعی, موش
Exercise training, MitoQ, atrophy, spinal cord injury, and rat.
8604
8616
http://jssu.ssu.ac.ir/browse.php?a_code=A-10-5372-1&slc_lang=fa&sid=1
Fatemeh
Rastegari Nasab Ravari
فاطمه
رستگارینسب راوری
frastgarinasabravari@gmail.com