fa بررسی فراوانی پلی‌مورفیسم‌های r‌s2274907 ژن Omentin و rs9770242 ژن Visfatin در ارتباط با بیماری کبد چرب غیر الکلی در یک جمعیت ایرانی عمومى General پژوهشي Original article <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:.1pt">مقدمه:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:.1pt">بیماری کبد چرب غیرالکلی رایج‌ترین بیماری مزمن کبدی است که در بسیاری از کشورهای شایع و در صورت عدم‌</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:.1pt">تشخیص و درمان تا ابتلا به سیروز کبدی و گاهی مرگ پیش خواهد رفت. این بیماری مرتبط با دیابت، چاقی، فشارخون، سندرم متابولیک و بیماری‌های قلبی و عروقی است. ژن امنتین دارای 8 اگزون و 7 اینترون می‌باشد که محصول پروتئینی آن شامل 313 اسیدآمینه است. </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">Visfatin</span></span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:.1pt"> یکی دیگر از آدیپوکین‌ها است. ژن </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">Visfatin</span></span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:.1pt"> بر روی بازوی بزرگ کروموزوم 7 قرار دارد. هدف از انجام این مطالعه بررسی ارتباط پلی‌مورفیسم های </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">rs2274907 Omentin</span></span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:.1pt"> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">rs9770242 Visfatin</span></span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:.1pt"> با بیماری کبد چرب غیرالکلی در یک جمعیت ایرانی است</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> این مطالعه به روش مورد </span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&ndash;</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> شاهدی با انتخاب 70 بیمار مبتلا به کبدچرب غیرالکلی و 70 فرد سالم انجام شد. میزان قندخون ناشتا، تری‌گلیسیرید، کلسترول،</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HD&nbsp; </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">،</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">LDL </span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&nbsp;با روش‌های استاندارد و میزان </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Omentin</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با کیت الایزا اندازه‌گیری شد. تعیین ژنوتیپ با روش </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">PCR-RFLP</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> انجام شد. داده‌ها توسط نرم‌افزار</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">version 16 </span></span>&nbsp;<span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">SPSS</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> تجزیه و تحلیل گردید.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">دو گروه مورد بررسی از نظر متغیرهای سن،</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">BMI </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">،</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">LDL </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">،</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">HDL </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، تفاوت معنی‌داری نشان داد. سطوح تری‌گلیسرید، </span></span><i><span dir="LTR" lang="EN" new="" roman="" style="font-family:" times="">Omentin</span></i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و میانگین نمایه توده بدنی‌، در افراد مبتلا به</span></span><span dir="LTR" new="" roman="" style="font-family:" times=""> NAFLD </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بالاتر و سطح </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">HDL-C </span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&nbsp;کم‌تر از افراد سالم بود. امنتین با نمایه توده بدنی </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">LDL-C</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">HDL-C</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> ارتباط معنی‌دار شد</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">. </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجه‌گیری:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> مطالعه حاضر نشان داد بین </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Omentin</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و بیماری کبدچرب غیر الکلی ارتباط معنی‌دار وجود دارد. هم‌چنین پلی‌مورفیسم </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">rs2274907</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در ژن </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Omentin</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با کبدچرب غیرالکلی ارتباط معنی‌داری دارد.</span></span></span></span></span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:17.0pt"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Introduction:</span></b> <span new="" roman="" style="font-family:" times="">Non-alcoholic fatty liver disease is the most common chronic disease, frequently occurring in various instances; if left undiagnosed and untreated, it will progress to cirrhosis and, in some cases, result in death. This disease is related to obesity, high blood pressure, metabolic syndrome and diseases and treatment. The Omentin gene consists of 8 exons and 7 introns, producing a protein made up of 313 amino acids. Visfatin is another adipokine. The Visfatin gene is located on the big arm of chromosome 7. The purpose of this study was to investigate the relationship between rs2274907 omentin and rs9770242 visfatin polymorphisms with non-alcoholic fatty liver disease in an Iranian population.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:17.0pt"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b> <span lang="EN" new="" roman="" style="font-family:" times="">This study was carried out through a case-control study involving 70 patients with non-alcoholic fatty liver disease and 70 healthy individuals. The level of fasting blood sugar, triglyceride, cholesterol, HDL, and LDL was measured by standard methods; the amount of <i>Omentin</i> was measured with an ELISA kit. Genotype was determined by PCR-RFLP method. The data were analyzed by SPSS software version 16.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:17.0pt"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><b> </b><span new="" roman="" style="font-family:" times="">The two investigated groups showed a significant difference in terms of age, BMI, LDL, HDL (P<0.05). The levels of triglycerides, omentin and average body mass profile were higher in the individuals with NAFLD; the level of HDL-C was lower than healthy individuals. Omentin had a significant relationship with the body mass level of LDL-C and HDL-C.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:17.0pt"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><b> </b><span new="" roman="" style="font-family:" times="">The current research demonstrated a notable connection between omentin and non-alcoholic fatty liver disease. This polymorphism of rs2274907 in the omentin gene has a significant relationship with non-alcoholic fatty liver disease.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br> &nbsp;</div> Omentin, آدیپوکین,آنزیم های کبدی , بیماری کبد چرب غیرالکلی Omentine, Adipokine, Liver enzymes. 8439 8450 http://jssu.ssu.ac.ir/browse.php?a_code=A-10-4882-3&slc_lang=fa&sid=1 Mahnaz Mohammadi مهناز محمدی mh_mohamadi@yahoo.com 0000-0002-7633-2162 Yes Department of Biology, Islamshahr Branch of Islamic Azad University, Islamshahr, Tehran, Iran. گروه زیست شناسی، واحد اسلامشهر دانشگاه آزاد اسلامی، اسلامشهر،تهران، ایران. Neda kolivand saloki ندا کولیوند سالوکی neda.kolivand03@gmail.com 0000-0002-5644-0968 No Department of Biotechnology, Faculty of Biotechnology, Medicine Branch, Azad University, Tehran, Iran. گروه بیوتکنولوژی، دانشگاه آزاد واحد علوم پزشکی تهران، ایران