fa اثر هشت هفته تمرین ترکیبی (مقاومتی- استقامتی) و مصرف مکمل تورین بر بیان پروتئین گیرنده‌های LXR و سطح سرمی کلسترول تام در موش‌های صحرایی نر ویستار دیابتی فیزیولوژی ورزش Exercise Physiology پژوهشي Original article <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:20.0pt"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b> <span dir="LTR" new="" roman="" style="font-family:" times="">Liver X Receptor</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> (</span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">LXR</span></i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">)</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">از</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ژن‌های</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">اصلی کبدی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">هموستاز</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">گلوکز</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">کلسترول</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">می&shy;‌باشد. دیابت باعث اختلال </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">LXR</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">کلسترول&shy;تام&shy; می&shy;‌شود. هدف، تعیین اثر هشت&shy;&shy;&shy;‌هفته تمرین‌&shy;&shy;ترکیبی (مقاومتی- استقامتی) و مکمل&shy;تورین بر</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> بیان&shy;</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&shy;</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">پروتئین گیرنده‌&shy;های </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">LXR</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">کلسترول‌&shy;&shy;تام سرمی در موش‌&shy;های&shy;&shy; صحرایی نر ویستار دیابتی بود. </span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">آزمودنی‌های این مطالعه&shy; تجربی، 50 سر موش&shy; صحرایی نر ویستار 6هفته‌&shy;ای با دامنه‌&shy;&shy;وزنی220-200 بود. جهت دیابتی&shy; کردن 40سر موش &shy;صحرایی، در پایان هشت&shy;‌هفتگی، 55 میلی&shy;گرم استرپتوزوسین (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">STZ</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) به&shy; ازای هر کیلوگرم وزن&shy; بدن موش‌های&shy; صحرایی با روش درون&shy;‌صفاقی تزریق شد و به&shy;‌صورت تصادفی در چهار&shy;گروه، تمرین&shy;‌ترکیبی (10=</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">n</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">)، تورین (10=</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">n</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">)، تمرین&shy;‌ترکیبی- تورین (10=</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">n</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) و کنترل (10=</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">n</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) قرار&shy; گرفتند. 10سر موش &shy;صحرایی سالم به&shy;‌عنوان کنترل&shy; سالم (10=</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">n</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) در نظر گرفته شد. گروه تمرین &shy;ترکیبی، تمرین &shy;&shy;مقاومتی شامل 15بار صعود از نردبان و تمرین&shy; استقامتی دویدن روی نوارگردان با شدت 75درصد</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Vo2max</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> را به&shy; مدت هشت &shy;هفته و 5بار در هفته انجام&shy; دادند. گروه&shy; تورین، محلول 1% مکمل&shy; تورین را در آب&shy;آشامیدنی روزانه دریافت&shy; کردند. در گروه تمرین &shy;ترکیبی- تورین مداخلات تمرین و تورین اعمال شد. برای مقایسه بین&shy;‌گروهی و درون&shy;‌گروهی، </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">ANOVA</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> دوراهه و آزمون&shy; تعقیبی </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">LSD</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> استفاده شد.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج</span></span></b><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در پایان هشت&shy;&shy; هفته، </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">LXR</span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> گروه‌&shy;های مکمل&shy; (0/0001=</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">P</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) و تمرین- مکمل&shy; (0/0001=</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">P</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) در مقایسه&shy; با کنترل کاهش معنی‌داری داشت. </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">LXR</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> گروه&shy;های تمرین-&shy; مکمل&shy; (0/0001=</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">P</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) و مکمل&shy; (0/011=</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">P</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) در مقایسه&shy; با تمرین کاهش معنی‌&shy;داری داشت. کلسترول گروه تمرین- &shy;مکمل&shy;&shy; (0/027=</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">P</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) در مقایسه &shy;با کنترل کاهش معنی‌&shy;داری داشت. وزن و </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">BMI</span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> گروه‌&shy;های تمرین، مکمل&shy; و تمرین-&shy; مکمل&shy; در &shy;مقایسه&shy; با کنترل&shy; کاهش معنی&shy;‌داری داشت. در حالیکه در گروه تمرین&shy; در &shy;مقایسه&shy; با گروه‌&shy;های مکمل&shy; و تمرین- مکمل&shy; افزایش معنی&shy;‌داری داشت (0/05></span></span><span dir="LTR" new="" roman="" style="font-family:" times="">P</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">).</span></span> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجه‌گیری:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">هشت&shy; هفته تمرین&shy; ترکیبی (مقاومتی- استقامتی) و مکمل &shy;تورین باعث کاهش گیرنده‌های </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">LXR</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، وزن، </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">BMI</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و کلسترول‌تام در موش‌های دیابتی شد.</span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:15.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">Introduction:</span></span></b> <span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">Liver X Receptor is a crucial gene in liver responsible for maintaining glucose and cholesterol homeostasis. Diabetes leads to disruption in LXR and total cholesterol disorder. The aim was to determine the effect of eight weeks of combined training (resistance-endurance) along with taurine supplementation on the expression of LXR receptors and serum total cholesterol in male Wistar rats</span></span><span new="" roman="" style="font-family:" times="">.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:15.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b><span new="" roman="" style="font-family:" times=""> The participants in this experimental study consisted of 50 male Wistar rats, each 6 weeks old, weighing between of 200-220 grams. To induce diabetes of 40 rats, at the end of the eighth week, the amount of 55 mg of streptozocin (STZ) per rat&rsquo;s body weight was injected intraperitoneally, and the rats were randomly divided into four groups: combined exercise (n=10), taurine (n = 10), combined training and taurine (n = 10) and control (n = 10). Ten healthy rats were considered as healthy controls. The combined training group completed resistance training, which included climbing the ladder 15 times, along with endurance workouts on the treadmill at an intensity of 75% Vo2max for eight weeks, 5 times each week. The taurine group received 1% taurine supplement solution in drinking water daily. Exercise and taurine interventions were applied in the combined exercise-taurine group. Two-way ANOVA along with the LSD post hoc test was employed to compare intergroup and intragroup.</span><b><span style="font-family:"Times New Roman",serif"></span></b></span></span></span><br> <span style="font-size:11pt"><span style="line-height:15.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><b> </b><span new="" roman="" style="font-family:" times="">After eight weeks, LXR levels decreased significantly in the supplement group (P=0.0001) and the exercise + supplementation group (P=0.0001) when compared to the control group. LXR showed a notable reduction in the exercise + supplement (P=0.0001) and supplement (P=0.011) groups when compared to the exercise group. Cholesterol levels decreased significantly in the exercise + supplement group (P=0.027) when compared to the control group. Body weight and BMI significantly were lower in the exercise, supplement and exercise + supplement groups in comparison to the control group. Although it was significantly higher in the exercise group when compared to the supplement and exercise + supplement groups (P<0.05).</span><b><span style="font-family:"Times New Roman",serif"></span></b></span></span></span><br> <span style="font-size:11pt"><span style="line-height:15.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><b> </b><span new="" roman="" style="font-family:" times="">Eight weeks of combined (resistance-endurance) training and taurine consumption decreased LXR, body weight, BMI and total cholesterol in diabetic rats.</span><b><span style="font-family:"Times New Roman",serif"></span></b></span></span></span><br> &nbsp;</div> تمرین مقاومتی- استقامتی, مکمل تورین, بیان پروتئین گیرنده LXR کبدی, کلسترول تام سرمی, موش‌های دیابتی Resistance-endurance training, Taurine supplementation, LXR protein expression, Serum total cholesterol, Diabetic rats. 8498 8511 http://jssu.ssu.ac.ir/browse.php?a_code=A-10-4070-4&slc_lang=fa&sid=1 Ameneh PourRahim Ghouroghchi آمنه پوررحیم قورقچی amenehpoorrahim@yahoo.com 0000-0003-3448-5950 Yes Department of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran. گروه فیزیولوژی ورزشی، دانشکده علوم تربیتی و روانشناسی، دانشگاه محقق اردبیلی، اردبیل، ایران. Aydin Valizadeh Orange آیدین ولیزاده اورنج A.valizadeh@uma.ac.ir 0000-0002-5890-4972 No Department of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran. گروه فیزیولوژی ورزشی، دانشکده علوم تربیتی و روانشناسی، دانشگاه محقق اردبیلی، اردبیل، ایران.