The Journal of Shahid Sadoughi University of Medical Sciences
مجله علمي پژوهشي دانشگاه علوم پزشكي شهید صدوقی يزد
JSSU
Medical Sciences
http://jssu.ssu.ac.ir
20
journal20
2228-5741
2228-5733
fa
jalali
1403
8
1
gregorian
2024
11
1
32
8
online
1
fulltext
fa
تاثیر تمرین تناوبی با شدت متوسط بر بیان ژنهای مرتبط با نکروپتوز بافت قلب موشهای نر مدل سکته قلبی
Effect of Moderate Intensity Interval Training on the Expression of Genes Related to Necroptosis of Heart Tissue in Male Heart Attack Model Rats
فیزیولوژی ورزش
Exercise Physiology
پژوهشي
Original article
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">آسیب ایسکمی - خونرسانی مجدد میوکارد مجموعهای از پاتو مکانیسمهای سلولی و مولکولی است که منجر به مرگ کاردیومیوسیتهای زنده و به خطر افتادن عملکرد قلب میشود. هدف از مطالعه حاضر تعیین تاثیر تمرین تناوبی با شدت متوسط بر بیان ژنهای </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RIPK-1</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RIPK-3</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MLKL</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> قلبی موشهای نر مدل سکته قلبی بود.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در مطالعه تجربی حاضر 30 سر موش صحرایی نر نژاد ویستار 16 هفتهای با وزن 220 تا 280 گرم بهطور تصادفی به سه گروه کنترل (سالم)، انفارکتوس میوکارد (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MI</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) و تمرین تناوبی با شدت متوسط تقسیم شدند. انفارکتوس میوکارد توسط مداخله مستقیم با انسداد </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در شریان نزولی قدامی چپ </span></span> <span dir="LTR" new="" roman="" style="font-family:" times="">(LAD)</span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> به مدت</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">¬</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">30 دقیقه انجام شد. پروتکل تناوبی با شدت متوسط 60 دقیقه دویدن تناوبی روی تردمیل، هر تناوب شامل 4 دقیقه دویدن با شدت 65 -70 درصد </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">VO2max</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و 2 دقیقه بازیافت فعال با شدت 50 </span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">–</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">60 درصد </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">VO2max</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، سه روز در هفته و به مدت 8 هفته انجام شد. به منظور بررسی بیان ژن متغیرهای تحقیق از روش </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">qRT-PCR</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> استفاده شد. دادهها با استفاده از نرم افزار </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">SPSS</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> ورژن 21 و روش آماری آنالیز واریانس یکطرفه و آزمون تعقیبی توکی در سطح معنیداری 0/05> </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> مقایسه شدند.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> بیان ژنهای </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RIPK-1</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RIPK-3</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MLKL</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> قلبی در گروه </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MI</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> نسبت به گروه کنترل بیشتر بودند؛ با اینحال، تمرین تناوبی با شدت متوسط در مقایسه با گروه </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MI</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> موجب کاهش بیان ژنهای </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RIPK-1</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RIPK-3</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MLKL</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> شد (به ترتیب با 0/001=</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و 0/006=</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">).</span></span> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجهگیری:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج تحقیق نشان داد انفارکتوس میوکارد با فعال شدن مسیر مرگ سلولی نکروپتوز همراه است، همچنین، تمرین تناوبی با شدت متوسط در کاهش بیان ژنهای نکروپتوز (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RIPK1</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RIPK3</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MLKL</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) تاثیر دارد. بااینحال، به دلیل کمبود شواهد و محدودیتهای تحقیق، همچنان نیازمند بررسیهای بیشتر میباشد.</span></span></span></span></span></span></span><br>
</div>
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Introduction:</span></b> <span new="" roman="" style="font-family:" times="">Myocardial ischemia-reperfusion injury is a set of cellular and molecular patho-mechanisms that lead to the death of living cardiomyocytes and compromised cardiac function. The aim of this study was to determine the effect of moderate intensity interval training on the expression of RIPK-1, RIPK-3 and MLKL genes in the heart of male heart attack model rats.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b><span new="" roman="" style="font-family:" times=""> In this experimental study, thirty 16-week-old male Wistar rats weighing 220 to 280 gr were randomly divided into three groups: control (healthy), myocardial infarction (MI), and moderate intensity interval training. Myocardial infarction was performed by direct intervention with occlusion in the left anterior descending artery (LAD) for 30 minutes. Interval training protocol was performed three days a week, for 8 weeks, with moderate intensity of 60 minutes of interval running on a treadmill, each interval including 4 minutes of running with an intensity of 65-70% of VO<sub>2</sub>max and 2 minutes of active recovery with an intensity of 50-60% of VO<sub>2</sub>max. qRT-PCR method was used to check the gene expression of research variables. Data were compared using SPSS software version 16, one-way analysis of variance and Tukey's post hoc test at a significance level of p<0.05.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><b> </b><span new="" roman="" style="font-family:" times="">The expression of cardiac RIPK-1, RIPK-3 and MLKL genes were higher in the MI group compared to the control group; however, moderate intensity interval training decreased the expression of RIPK-1, RIPK-3 and MLKL genes compared to the MI group (p=0.001 and p=0.006, respectively).</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><b> </b><span new="" roman="" style="font-family:" times="">The results of the research showed that myocardial infarction is associated with the activation of the necroptosis cell death pathway, likewise, interval training with moderate intensity has an effect on reducing the expression of necroptosis genes (RIPK1, RIPK3, and MLKL). However, due to the lack of evidences and limitations of research, it still needs more investigations.</span><b><span style="font-family:"Times New Roman",serif"></span></b></span></span></span><br>
</div>
انفارکتوس میوکاردی, تمرین تناوبی, نکروپتوز قلبی
Myocardial infarction, Interval training, Cardiac necroptosis.
8173
8186
http://jssu.ssu.ac.ir/browse.php?a_code=A-10-5342-1&slc_lang=fa&sid=1
Negar
Rostami
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رستمی
rostami.n86@gmail.com