fa بررسی اثرات ضدسرطانی نانو‌ذره نیوزوم سنتزشده با استفاده از عصاره گیاه Artemisia anuua و آنالیز بیان ژن‌های آپوپتوزی Caspase3 و Caspase9 بر رده سلولی سرطان پستان ژنتیک Genetics پژوهشي Original article <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#222222">فرمولاسیون دارویی نانوذرات به دلیل دستیابی به داروی رهاسازی هدفمند و پایدار، بهبود حلالیت دارو و کاهش واکنش‌های جانبی دارویی، کاربرد زیادی دارد. یکی از راه‌های بهبود بقای بیماران سرطانی، درمان با استفاده از نانوحامل‌ها برای داروهای ضد سرطان است.</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> هدف از این مطالعه، سنتز نیوزوم آنکپسوله شده حاوی عصاره گیاه درمنه و بررسی اثرات ضد سرطانی و آپوپتوزی علیه رده سلولی سرطان پستان است.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در این مطالعه ابتدا نانوحامل نیوزومی ساخته و عصاره گیاه درمنه گندواش در </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">آ</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ن بارگزاری شد. خصوصیات فیزیکی و شیمیایی آن با استفاده از میکروسکوپ الکترونی </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">SEM</span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و پراکندگی نور دینامیکی (</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">DLS</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) و پتانسیل زتا</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مورد تایید قرار گرفت. هم‌چنین درصد محصورسازی و الگوی رهایش عصاره بررسی شد. در انتها اثرات ضد سرطانی آن توسط روش رنگ سنجی </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">MTT</span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> برعلیه رده سلولی سرطان پستان </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">(MCF_7)</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> بررسی شد و میزان بیان </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ژن‌های آپوپتوزی </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">Caspase3</span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">Caspase9</span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با استفاده از روش </span></span><b>&nbsp;</b><span dir="LTR" new="" roman="" style="font-family:" times="">Real Time PCR</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مورد مطالعه قرار گرفت. در انتها آنالیز آماری توسط نرم افزار </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">GraphPad Prism</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&nbsp; انجام شد و داده های سمیت سلولی و بیان ژن با آنالیز توسط واریانس یک طرفه (</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">one-way analysis of variance </span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) مورد بررسی قرار گرفت. </span></span><span dir="LTR" style="font-family:"Times New Roman",serif"></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">یافته‌ها نشان داد که نانوحامل نیوزوم سنتزشده حاوی عصاره، دارای ساختار کروی می‌باشد و اندازه آن 208/1نانومتر و</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">درصد محصورسازی 62/35%&nbsp; و شارژ سطحی 22/5</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">- است. هم</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">‌</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">چنین نانوحامل نیوزوم حاوی عصاره، دارای اثرات ضدّسرطانی معنا داری نسبت به عصاره آزاد می باشد</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و به ترتیب باعث افزایش بیان ژن‌های </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">caspase3 </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">caspase9</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> به میزان 0/34</span></span></span></span></span></span></span><span style="font-size:16px;">&plusmn;</span><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> </span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span><span dir="LTR" style="font-family:"Times New Roman",serif"></span><span style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span>&nbsp;<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&nbsp;1/184</span></span><span dir="LTR" new="" roman="" style="font-family:" times=""> (P<0/05) </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و0/81 </span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span><span dir="LTR" style="font-family:"Times New Roman",serif"></span><span style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span><span style="font-size:11.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span dir="RTL" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span> <span style="font-size:16px;">&plusmn;</span><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> 1/68</span></span><span dir="LTR" new="" roman="" style="font-family:" times=""> (P<0/05)</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> می شود.</span></span> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجه‌گیری:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج نشان داد که نیوزوم حاوی عصاره گیاه درمنه گندواش اثرات ضد سرطانی و آپوپتوزی را به‌طور معناداری افزایش می‌دهد و بنابراین می‌توان با مطالعات بیشتر در آینده از نیوزوم به عنوان سیستم دارورسانی هدفمند جهت اهداف ضدسرطانی استفاده نمود.</span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Introduction:</span></b> <span new="" roman="" style="font-family:" times="">Pharmaceutical formulation of nanoparticles is widely used due to achieving targeted and stable drug release, improving drug solubility and reducing side drug reactions. One of the ways to improve the survival of cancer patients is the treatment using nanocarriers for anticancer drugs. The aim of this study was to synthesize encapsulated nisomes containing the extract of the Artemisia annua and investigate the anticancer and apoptotic effects against breast cancer cell lines.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b> <span new="" roman="" style="font-family:" times="">In this study, a Niosomes nanocarrier was made and then the extract of Gondwash herb was loaded into it. Its physical and chemical properties were confirmed using SEM, dynamic light scattering (DLS) and zeta potential. Likewise, the encapsulation percentage and release pattern of the extract were investigated. Finally, its anticancer effects against the breast cancer cell line (MCF_7) were investigated and the expression level of apoptotic genes Caspase3 and Caspase9 was studied</span> <span new="" roman="" style="font-family:" times="">using Real Time PCR method. At the end, statistical analysis was done by GraphPad Prism software, and cytotoxicity and gene expression data were analyzed by one-way analysis of variance.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><b> </b><span new="" roman="" style="font-family:" times="">The findings showed that the synthesized nanocarrier newsom containing the extract had a spherical structure and its size was 208.1 nanometers, the encapsulation percentage was 62.35% and the surface charge was 22.5. Similarly, newsome nanocarrier containing the extract had significant anticancer effects compared to the free extract and increased the expression of caspase 3 and caspase 9 genes by 1.184 &plusmn; 0.34 (P<0.05) and 1.68 &plusmn; 0.81, respectively. (P<0.05).</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><b> </b><span new="" roman="" style="font-family:" times="">The results showed that the nisome containing the extract of the Gondwash herb increases the anti-cancer and apoptotic effects significantly, and therefore, with further studies in the future, nisome can be used as a targeted drug delivery system for anti-cancer purposes.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> &nbsp;</div> نانوذره, نیوزوم ,Artemisia anuua, ضد سرطانی, سرطان پستان Nanoparticle, Nisome, Artemisia anuua, Anticancer, Breast cancer. 8019 8032 http://jssu.ssu.ac.ir/browse.php?a_code=A-10-5159-1&slc_lang=fa&sid=1 Zahra Rastgar زهرا رستگار zahrarastgar9@gmail.com 0000-0002-3665-5442 No Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran گروه زیست‌شناسی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران. Fariba Khosravi-Nejad فریبا خسروی نژاد f.khosravinejad@iau-tnb.ac.ir 0000-0003-3880-4162 Yes Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran گروه زیست‌شناسی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران.