fa بررسی اثر تمرین مقاومتی - هوازی بر بیومارکرهای جذبی و بازجذبی بافت استخوانی در موش‌های نر کودک، بالغ و سالمند عمومى General پژوهشي Original article <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تمرینات ورزشی احتمالاً به عنوان یک مداخله غیردارویی برای بهبود سلامت استخوان در کودکان و سالمندان موثر باشد. هدف از پژوهش حاضر، بررسی اثر تمرین مقاومتی - هوازی بر بیومارکرهای </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RUX2</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">CTX</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">،</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RANKL </span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&nbsp;و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">ALP</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در موش‌های با سنین مختلف بود.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">درمطالعه تجربی حاضر، 30 سر موش صحرایی نر </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نژاد ویستار از انستیتوپاستور ایران تهیه و</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بر اساس رده سنی در سه گروه؛ کودک (2هفته&shy;ای)، بالغ (6هفته&shy;ای) و سالمند (96هفته&shy;ای) قرارگرفتند. </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در هر رده سنی موش‌&shy;ها به صورت تصادفی در دو گروه تمرین (5</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">(n=</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و کنترل </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">)</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">5</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">(n=</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> تقسیم شدند. </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">6 هفته تمرینات ترکیبی شامل سه جلسه تمرین هوازی و سه جلسه تمرین مقاومتی در هفته بود</span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">.</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">برای اندازه&shy;گیری بیان ژن&shy;ها از روش </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">PCR</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> استفاده شد.</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">برای تجزیه و تحلیل داده&shy;ها، آنالیز واریانس یک طرفه و آزمون تعقیبی توکی و نرم‌افزار</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">version 16 </span></span>&nbsp;<span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">SPSS</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> استفاده شد و</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> سطح&shy;معنی&shy;داری (0/05</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&ge;</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) در نظر گرفته شد.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> آنالیز واریانس یک طرفه نشان داد که تمرین مقاومتی - هوازی بر متغیرهای</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RUX2 </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RANKL</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">ALP</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> موش‌های صحرایی در رده‌های سنی مختلف اثر معنی‌داری دارد (0/05 > </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">)؛ اما این معنی‌داری در متغیر </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">CTX</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> مشاهده نشد (0/05 < </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">). از طرفی نتایج آزمون توکی </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RUX2</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> بین گروه‌های تمرین بالغ و تمرین سالمند (0/019=</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) هم‌چنین بین گروه‌های کنترل سالمند و تمرین بالغ (0/002=</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) تفاوت معنی‌داری داشت. درخصوص متغیر </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RANKL</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> نشان داد که تنها بین گروه تمرین بالغ و کنترل سالمند تفاوت معنی‌دار وجود دارد (0/01= </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">).</span></span> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجه‌گیری:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تمرین مقاومتی - هوازی احتمالاً سبب افزایش بیومارکرهای جذبی و بازجذبی از قبیل </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RUX2</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RANKL</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">ALP</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> موش</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span><span b="" nazanin="" style="font-family:">های نر کودک، بالغ و سالمند می‌شود.</span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><span style="font-family:"Times New Roman",serif"></span><b><span new="" roman="" style="font-family:" times="">Introduction:</span></b> <span new="" roman="" style="font-family:" times="">Exercise training is likely to be a non-pharmacological intervention to improve bone health in children and the elderly. The aim of this study was to investigate the effect of resistance-aerobic training on RUX2, CTX, RANKL and ALP biomarkers in rats of different ages.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b><span new="" roman="" style="font-family:" times=""> In the present experimental study, 30 male Wistar rats were obtained from Instituto Pasteur of Iran and were divided into three groups according to their age: immature (2 weeks old), mature (6 weeks old) and elderly (96 weeks old). In each age group, the mice were randomly divided into two training (n=5) and control (n=5) groups. Six weeks of combined training included three sessions of aerobic training and three sessions of resistance training per week. Extracting genes by PCR method was used. One-way analysis of variance and Tukey&#39;s post hoc test and SPSS16</span> <span new="" roman="" style="font-family:" times="">software were used. The significance level P&ge;0.05 was considered.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><b> </b><span new="" roman="" style="font-family:" times="">One-way analysis of variance showed that resistance-aerobic training has had a significant effect on RUNX2, RANKL and ALP variables of rats in different age groups (P < 0.05). This significance was not observed in CTX variable (P > 0.05). On the other hand, the results of Tukey&#39;s RUNX2 test were significantly different between the adult training and elderly training groups (p = 0.019) as well as between the elderly control groups (p = 0.01) and adult training (p = 0.002). Regarding the RANKL variable, it showed that there was a significant difference only between the adult exercise group and the elderly control group (p = 0.01).</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion: </span></b><span new="" roman="" style="font-family:" times="">Resistance-endurance training probably increases absorption and reabsorption biomarkers such as RUX2, RANKL and ALP in immature, mature and elderly male rats.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> &nbsp;</div> تمرین مقاومتی, RUX2, RANKL, ALP و CTX Resistance training, RUX2, RANKL, ALP and CTX. 7505 7515 http://jssu.ssu.ac.ir/browse.php?a_code=A-10-4564-4&slc_lang=fa&sid=1 Mahshid Shiri مهشید شیری Shirim258@gail.com 0000-0002-3592-4161 No Department of Physical Education and Sports Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran. گروه تربیت بدنی و علوم ورزشی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران Mohammad Reza Fadai Chafi محمدرضا فدائی چافی mfadaei2000@yahoo.com 0000-0003-0265-5329 Yes Department of Physical Education and Sports Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran. گروه تربیت بدنی و علوم ورزشی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران Shahram Gholamrezaei شهرام غلامرضایی gholamrezaei@iaurasht.ac.ir 0000-0003-3105-6819 No Department of Physical Education and Sports Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran. گروه تربیت بدنی و علوم ورزشی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران