fa بررسی همراهی چندشکلی ژن TP53 (rs12602273C>G)با استعداد ابتلا به سرطان پستان در شمال غرب ایران ژنتیک Genetics پژوهشي Original article <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ژن</span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">TP53</span></span></i> <span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&nbsp;بر روی بازوی کوتاه کروموزوم 17 که به‌عنوان ژن سرکوب‌کننده تومور شناخته شده است، کد‌کننده فاکتور رونویسی </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p53</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> می‌باشد و این فاکتور پیشرفت چرخه سلولی را کنترل می‌کند. این مطالعه با هدف بررسی همراهی چندشکلی </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">rs12602273 C>G</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> ژن </span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">TP53</span></span></i><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با استعداد ابتلا به سرطان پستان در جمعیت شمال غرب ایران انجام شد</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">.</span></span><b><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span></b></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در این مطالعه مورد - شاهدی، همراهی چندشکلی </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">rs12602273</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> ژن </span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">TP53</span></span></i><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با استعداد ابتلا به سرطان پستان در جامعه آماری متشکل از 100 مورد بیمار و 100 فرد سالم با تکنیک </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Tetra ARMS-PCR</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> مورد بررسی قرار گرفت. تجزیه و تحلیل داده</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&shy;</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های حاصل با استفاده از نرم‌افزار</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">version 16 </span></span>&nbsp;<span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">SPSS</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و برنامه‌ آماری آنلاین </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">javastat online statistics package</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> انجام ‌شد.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> فراوانی ژنوتیپ</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&shy;</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">CC</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">CG</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">GG</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در بیماران به ترتیب 81/52، 16/30 و 2/17 درصد و فراوانی آن</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&shy;</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ها در افراد سالم مورد مطالعه به ترتیب 79/34، 17/39 و3/26 درصد بود. فراوانی آلل</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&shy;</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">C</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">G</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در افراد بیمار به ترتیب 89/67 و 10/32 درصد بود. در جمعیت کنترل فراوانی آلل</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&shy;</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">C</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">G</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در افراد بیمار به ترتیب 88/04 و 11/95 درصد بود. تجزیه</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&shy;</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های آماری نشان دهنده عدم همراهی فراوانی ژنوتیپی (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> ></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">0.05</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) و آللی (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">0.05</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) چندشکلی (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">rs12602273C>G</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) ژن </span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">TP53</span></span></i><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با سرطان پستان بود. هم‌چنین نتایج حاصل نشان‌دهنده عدم همراهی چندشکلی مورد مطالعه با ویژگی</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&shy;</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های بالینی بیماران مبتلا به سرطان پستان در زنان شمال</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&not;</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">غرب ایران بود </span></span></span></span></span></span></span>(P>0.05).<br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجه‌گیری:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">براساس نتایج حاصل چندشکلی</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">rs12602273C>G</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> ژن با </span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">TP53</span></span></i><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با افزایش ریسک ابتلا به سرطان پستان همراهی ندارد. همراهی توزیع ژنوتیپی این </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">SNP</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با ویژگی</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&shy;</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های بالینی بیماران غیرمعنی</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">دار بود.</span></span></span></span></span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Introduction:</span></b> <span new="" roman="" style="font-family:" times="">The</span><span new="" roman="" style="font-family:" times=""> <i>TP53</i> gene on </span><span new="" roman="" style="font-family:" times="">the short arm of </span><span new="" roman="" style="font-family:" times="">chromosome 17p, which is known as a tumor suppressor gene, encodes the transcription factor <i>p53</i>, and this factor controls cell cycle progression. This study was done to assess the association of <i>TP53</i> (rs12602273C>G) gene polymorphism with the risk and prognosis of breast cancer in women of northwest of Iran.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b> <span new="" roman="" style="font-family:" times=""><span style="color:black">In this case-control study, the association of <i>TP53</i></span></span> <span new="" roman="" style="font-family:" times=""><span style="color:black">(rs12602273C>G) gene polymorphism with breast cancer risk was studied in a population, including 100 patients and 100 healthy individuals was investigated by Tetra ARMS-PCR </span></span><span new="" roman="" style="font-family:" times="">technique</span><span new="" roman="" style="font-family:" times=""><span style="color:black">. </span></span><span new="" roman="" style="font-family:" times="">The analysis of the resulting data was done using SPSS version 16 software and JavaStat online statistics package.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><b> </b><span new="" roman="" style="font-family:" times="">In the case group, the frequency of CC, CG, and GG genotypes were 81.52, 16.3, 2.17 percent, respectively and they were 79.34, 17.39, and 3.26 percent for the control group. Similarly, C and G allele frequency in case group was 89.67 and 10.32 percent and those in the control group was 88.04 and 11.95 percent, respectively. Statistical analyzes showed no association between genotypic (P>0.05) and allelic (P>0.05) polymorphism (rs12602273C>G) of TP53 gene with breast cancer. Also, the results showed that the studied polymorphism was not associated with the clinical characteristics of breast cancer patients in the Northwest of Iran (P>0.05). </span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><b> </b><span new="" roman="" style="font-family:" times="">These research findings suggest that <i>TP53</i> polymorphism of rs12602273C>G is not related to the risk of breast cancer. The association of the genotypic distribution of this SNP with the clinical characteristics of the patients was insignificant.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> &nbsp;</div> سرطان پستان, چند‌شکلی تک نوکلئوتیدی, ژن TP53, Tetra ARMS-PCR Breast cancer, Single nucleotide polymorphism, TP53 gene, Tetra ARMS-PCR. 7416 7427 http://jssu.ssu.ac.ir/browse.php?a_code=A-10-5203-1&slc_lang=fa&sid=1 Fatemeh Lotfi Dashbelagh فاطمه لطفی داشبلاغ lotfi.azaruniv.biology95@gmail.com 0009-0008-1692-3412 No Department of Biology, Faculty of Basic Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran. گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه شهید مدنی آذربایجان، تبریز، ایران. Sara Ghaffarian سارا غفاریان s.ghaffarian@azauniv.ac.ir 0000-0003-0385-8638 Yes Department of Biology, Faculty of Basic Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran. گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه شهید مدنی آذربایجان، تبریز، ایران.