The Journal of Shahid Sadoughi University of Medical Sciences
مجله علمي پژوهشي دانشگاه علوم پزشكي شهید صدوقی يزد
JSSU
Medical Sciences
http://jssu.ssu.ac.ir
20
journal20
2228-5741
2228-5733
fa
jalali
1402
9
1
gregorian
2023
12
1
31
9
online
1
fulltext
fa
تعیین فراوانی ژنهای تولیدکننده کلیسینهای Ia و V در ایزولههای Escherichia coli جدا شده از نمونههای بالینی مراکز درمانی شهر یزد
Determining the Frequency of Genes Producing Colicins Ia and V in Escherichia coli Strains Isolated from Clinical Samples of Medical Centers in Yazd City
میکروبیولوژی
Microbiology
پژوهشي
Original article
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">باکتریوسینها پپتیدهایی با خاصیت ضد میکروبی میباشند. آن</span></span><span dir="LTR" lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">‎</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ها به صورت ریبوزومال سنتز شده و توسط هر دو گروه از باکتریهای گرم مثبت و گرم منفی تولید میشوند. باکتریوسینهای تولید شده توسط </span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">Escherichia coli</span></i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">،</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> کلیسین نام دارند. کلیسین</span></span><span dir="LTR" lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">‎</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ها در باکتری</span></span><span dir="LTR" lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">‎</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های کومنسال، مانع از رشد باکتری</span></span><span dir="LTR" lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">‎</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های پاتوژن می</span></span><span dir="LTR" lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">‎</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">شوند و در سویه</span></span><span dir="LTR" lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">‎</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های پاتوژن باعث افزایش ویرولانس باکتری می</span></span><span dir="LTR" lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">‎</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">گردند. هدف از مطالعه حاضر تعیین فراوانی ژنهای </span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">col V</span></i> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و </span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">col Ia</span></i> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در نمونههای </span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">Escherichia coli</span></i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> جدا شده از بیماران با عفونت ادراری در شهر یزد است.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در این مطالعه توصیفی - مقطعی، تعداد 160 جدایه </span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">Escherichia coli</span></i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> از نمونه ادرار بیماران جدا شد و با آزمایشات بیوشیمیایی رایج شناسایی گردید. سپس واکنش </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">PCR</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> جهت بررسی فراوانی ژنهای تولیدکننده </span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">col V</span></i> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و </span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">col Ia</span></i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با استفاده از پرایمرهای اختصاصی انجام شد و پس از تعیین توالی، نتایج با استفاده از نرمافزار</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">version 16 </span> <span dir="LTR" new="" roman="" style="font-family:" times=""> SPSS</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">واکاوی گردید.</span></span><span lang="FA" style="font-family:"Arial",sans-serif"></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در این مطالعه از میان 160 جدایه </span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">Escherichia coli</span></i> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مورد بررسی، ژن کلیسین در26/9 درصد از جدایهها شناسایی شد. فراوانی ژن </span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">col Ia (</span></i><span dir="LTR" new="" roman="" style="font-family:" times="">4/24</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">درصد) و فراوانی ژن </span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">col V</span></i><span dir="LTR" new="" roman="" style="font-family:" times=""> (6/10</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">درصد) بهدست آمد. همچنین در 8/12 درصد از جدایه</span></span><span dir="LTR" lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">‎</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ها، هر دو ژن مورد بررسی با هم شناسایی گردید. فراوانی ایزولههایی که فقط ژن </span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">col Ia</span></i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> را داشتند، 16/2درصد و ایزولههایی که فقط ژن </span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">col V</span></i> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">را داشتند، 2/5 درصد بود.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجهگیری:</span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در این بررسی، جدایههای دارای ژن </span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">col Ia</span></i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> فراوانی بالایی را نشان دادند. اغلب سویههای دارای ژن </span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">col V</span></i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، دارای ژن </span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">col Ia</span></i> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نیز بودند. بنابراین ژن </span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">col V</span></i> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">اغلب همراه ژن </span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">col Ia</span></i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> مشاهده میشود.</span></span></span></span></span></span></span><br>
</div>
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Introduction:</span></b> <span new="" roman="" style="font-family:" times="">Bacteriocins are peptides with antimicrobial properties. They are ribosomally synthesized and produced by both gram-positive and gram-negative bacteria. The bacteriocins produced by <i>Escherichia coli</i> are called colicins. Colicins in commensal bacteria inhibit the growth of pathogenic bacteria and in pathogen strains, they increase bacterial virulence. The aim of this study was to evaluate the frequency of <i>col V </i>and <i>col Ia </i>genes in <i>Escherichia coli </i>specimens isolated from patients with urinary tract infections in Yazd City, Iran</span><span new="" roman="" style="font-family:" times="">.</span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b> <span new="" roman="" style="font-family:" times="">In this descriptive cross-sectional study, 160 <i>Escherichia coli</i> samples were isolated from urine specimens and identified by common biochemical tests. The amplification of <i>col V</i> and <i>col Ia</i> genes was performed by PCR method using specific primers and after sequencing, the results were analyzed using SPSS version 16 software.</span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><b> </b><span new="" roman="" style="font-family:" times="">In this study, out of 160 Escherichia coli isolates examined, calicin gene was detected in 26.9% of the isolates.</span><b> </b><span new="" roman="" style="font-family:" times="">The frequency of <i>col Ia</i> gene was 24.4% and the frequency of <i>col V</i> gene was 10.6%. Furthermore, in 8.12% of isolates, both genes were identified together. The frequency of isolates containing only <i>col Ia</i> gene was 16.2% and isolates containing only <i>col V</i> gene was 2.5%.</span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><b> </b><span new="" roman="" style="font-family:" times="">In this study, isolates with <i>col Ia</i> gene showed high frequency. Most strains with <i>col V</i> gene also had <i>col Ia </i>gene. Therefore, the <i>col V</i> gene is often observed together with the <i>col Ia</i> gene.</span></span></span></span><br>
</div>
اشرشیاکلی, باکتریوسین, کلیسین
Escherichia coli, Bacteriocin, Colicin.
7062
7069
http://jssu.ssu.ac.ir/browse.php?a_code=A-10-1741-3&slc_lang=fa&sid=1
Habibollah
Zare
حبیب اله
زارع
h.zare1386@gmail.com