The Journal of Shahid Sadoughi University of Medical Sciences
مجله علمي پژوهشي دانشگاه علوم پزشكي شهید صدوقی يزد
JSSU
Medical Sciences
http://jssu.ssu.ac.ir
20
journal20
2228-5741
2228-5733
fa
jalali
1402
9
1
gregorian
2023
12
1
31
9
online
1
fulltext
fa
بررسی ایمونوهسیتوشیمیایی بروز MDM2 در ادنتوژنیک کراتوسیست و کیست دنتی ژروس
Immunohistochemical Study of MDM2 Expression in Odontogenic Keratocyst and Dentigerous Cyst
پاتولوژی
Pathology
پژوهشي
Original article
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">کیست دنتی ژروس و ادنتوژنیک کراتوسیست </span></span><span dir="LTR" new="" roman="" style="font-family:" times=""> (OKC)</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">دو تا از شایعترین کیستهای رشدی تکاملی ادنتوژنیک میباشند. مکانیسم رشدی و رفتار بیولوژیکی متفاوت</span></span><span dir="LTR" new="" roman="" style="font-family:" times=""> OKC </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">به علت فاکتورهای ناشناخته ذاتی دراپیتلیوم یا فعالیت آنزیماتیک دیواره آن است</span></span><span dir="LTR" new="" roman="" style="font-family:" times=""> P53. </span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در تنظیم مسیرهای ترمیم،</span></span><span dir="LTR" new="" roman="" style="font-family:" times=""> DNA </span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> آپوپتوز، آنژیوژنز و حفظ ثبات ژنومی نقش دارد و بهصورت منفی بهوسیله تقابل با</span></span><span dir="LTR" new="" roman="" style="font-family:" times=""> (Murine double minute2 ) MDM2 </span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> تنظیم میگردد. هدف این مطالعه بررسی بروز</span></span><span dir="LTR" new="" roman="" style="font-family:" times=""> MDM2 </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بهعنوان نشانگر مهم تکثیر و تنظیم چرخه سلولی در</span></span><span dir="LTR" new="" roman="" style="font-family:" times=""> OKC </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و کیست دنتی ژروس میباشد</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در این مطالعه توصیفی - مقطعی، بروز پروتئین</span></span><span dir="LTR" new="" roman="" style="font-family:" times=""> MDM2 </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در مناطق سوپرابازال و بازال اپیتلیوم 15 نمونه از هر یک از کیستهای دنتی ژروس و</span></span> <span dir="LTR" new="" roman="" style="font-family:" times="">OKC </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">با استفاده از تکنیک ایمونوهیستوشیمی بررسی شد. در نهایت دادهها وارد نرمافزار</span></span><span dir="LTR" new="" roman="" style="font-family:" times=""> SPSS version 16 </span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> شده و با استفاده از آزمون</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">آماری </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">t-test </span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> آنالیز شد</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">.</span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بروز </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MDM2 </span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در هر دو ناحیه بازال و سوپرابازال در </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">OKC</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> بالاتر از کیست دنتی ژروس بود؛ اما ارتباط معنیداری دیده نشد (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P:0.551) </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">،</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">(P: 0.825</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> ) .میزان بیان این مارکر در هر دو گروه مورد بررسی در ناحیه سوپرابازال به طور معنیداری بالاتر از ناحیه بازال بود(</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P: 0.005) </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">(P: 0.004</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">).</span></span> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجهگیری:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بیان بالاتر </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MDM2</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">OKC</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> بهخصوص در نواحی سوپرابازال میتواند نشاندهنده نقش ثانویه این پروتئین در پاتوژنز، رشد و گسترش این کیست باشد و فرآیندهای تکثیر، آپوپتوز و تمایز، تأییدکننده ماهیت نئوپلاستیک و رفتار بیولوژیک خاص این ضایعه میباشد.</span></span></span></span></span></span></span><br>
</div>
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">Introduction:</span></span></b> <span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">Dentigerous cyst and odontogenic keratocyst (OKC) are two of the most common developmental odontogenic cysts. Different developmental mechanism and biological behavior of OKC is due to unknown factors inherent in the epithelium or enzymatic activity in the wall. P53 plays a role in determining the pathways for DNA repair، apoptosis، angiogenesis and genomic stability and is negatively confronted with the MDM2 oncoprotein. Aim of this study was to evaluate the expression of MDM2 as an important indicator of proliferation and cell cycle regulation in OKC and dentigerous cyst.</span></span><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt"></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b><span new="" roman="" style="font-family:" times=""> In this descriptive-cross-sectional study، MDM2 expression in the suprabasal and basal epithelium regions of 15 samples from each of the dentigerous cysts and OKC was assessed using immunohistochemistry. Finally، the data were entered into the SPSS16 software and analyzed using the t-test.</span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><b> </b><span new="" roman="" style="font-family:" times="">MDM2 expression in the suprabasal and basal epithelium regions in OKC was higher than dentigerous cyst، but there was not seen a statically significant difference (P: 0.551)، (P: 0.825). The expression level of this marker in both groups in the suprabasal region was significantly higher than the basal region (P: 0.005)، (P: 0.004).</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><b> </b><span new="" roman="" style="font-family:" times="">Higher expression of MDM2 in OKC، especially in the suprabasal areas، could indicate a secondary role of this protein in the pathogenesis، growth and spread of this cyst and the proliferation، apoptosis and differentiation processes confirm the neoplastic nature and specific biological behavior of this lesion.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br>
</div>
MDM2, کیست دنتی ژروس, ادنتوژنیک کراتوسیست
MDM2, Dentigerous cyst, Odontogenic keratocyst.
7022
7029
http://jssu.ssu.ac.ir/browse.php?a_code=A-10-3120-1&slc_lang=fa&sid=1
Najmeh
Jafari
نجمه
جعفری
jafarynajmeh@yahoo.com