fa بررسی اثر ضد باکتریایی نانوذره هیبریدی سلنیوم و عصاره خیار دریایی بر باکتری اشریشیا‌کلی، میکروکوکوس لوتئوس و باسیلوس سرئوس فارماکولوژی Pharmacology پژوهشي Original article <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">خیار</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">دریایی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">یکی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">از</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">جانوران</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مهم</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">آبزی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">است</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">که</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">دارای</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">خوا</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ص </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تغذیه</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">‌</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ای </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> دارویی</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">فراوان </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">می‌باشد و به دلیل</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">حضور ترکیباتی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">با</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">اثر</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">درمانی و</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ضد</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">باکتریایی دارای</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">اهمیت</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">پزشکی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">می‌باشد</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">.</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> هدف از این مطالعه </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بررسی اثر ضد باکتریایی نانوذره هیبریدی سلنیوم و عصاره خیار دریایی بر باکتری ا</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">شریشیا کلی</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، میکروکوکوس لوتئوس و باسیلوس سرئوس</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> می‌باشد.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ارزیابی</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> MIC<b> </b></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">برای</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">اثر سیتوتوکسیسیته عصاره خیار دریایی </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بر</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سلول‌های</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">میکروبی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تعیین</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">شد.</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> پس از جمع‌آوری و عصاره‌گیری خیارهای دریایی، با لستفاده از روش</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">MIC </span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&nbsp;اثر ضد باکتریایی عصاره خیار دریایی، نانوذره هیبریدی سلنیوم و اثر توام عصاره خیار دریایی و نانوذره هیبریدی سلنیوم بر روی باکتری های ا</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">شریشیا کلی</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، میکروکوکوس لوتئوس و باسیلوس سرئوس</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> مورد ارزیابی قرار گرفت.</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تجزیه ‌و تحلیل آماری با استفاده از نرم‌افزار </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">SPSS version 16</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، انجام شد</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج آزمایش</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">‌</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های انجام شده نشان داد که عصاره</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">خیار</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">دریایی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">با</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">رقت</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">‌</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مختلف</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بر</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">رو</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">باکتری </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ا</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">شریشیا کلی</span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">‌</span></i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، باکتری </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">میکروکوکوس لوتئوس</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و باکتری</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">باسیلوس</span></span><i> </i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سرئوس</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">دارای اثر ضد باکتریایی بود</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">. </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">هم</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">‌</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">چنین</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">این</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">برای</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تست</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">‌</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">انجام</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">شده</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">با</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نانو داروی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سلنیوم</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نیز</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">صدق</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">می‌کرد.</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">جالب</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">توجه</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">است</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">که</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">پس</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">از</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ترکیب</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقدار</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مشخصی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">از</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">عصاره</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">خیار</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">دریایی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نانوداروی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سلنیوم</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">اثر ضد باکتریایی بیشتری بر روی</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">3</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">باکتری</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">فوق الذکر</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&nbsp;مشاهده شد</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">.</span> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجه‌گیری:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">اثر سینرژیستیک عصاره خیار دریایی و نانوذره سلنیوم دارای میزان بالاتری از خاصیت آنتی‌باکتریال است.</span></span></span></span></span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Introduction:</span></b> <span new="" roman="" style="font-family:" times="">Sea cucumber is one of the important aquatic animals that has many nutritional and medicinal properties and is of medical importance due to the presence of compounds with therapeutic and antibacterial effects. The purpose of this study was to investigate the antibacterial effect of selenium hybrid nanoparticles and sea cucumber extract on Escherichia coli, Micrococcus luteus and Bacillus cereus bacteria.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b><span new="" roman="" style="font-family:" times=""> MIC evaluation method was determined for the cytotoxic effect of sea cucumber extract on microbial cells. To carry out this research, after collecting and extracting sea cucumbers, the cytotoxicity of this extract on microbial cells was determined using the MIC method. Then, the antibacterial effect of sea cucumber extract, selenium hybrid nanoparticle and the combined effect of sea cucumber extract and selenium hybrid nanoparticle on Escherichia coli, Micrococcus luteus and Bacillus cereus bacteria were evaluated.</span> <span new="" roman="" style="font-family:" times="">Statistical analysis was done using SPSS version 16 software.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><b> </b><span new="" roman="" style="font-family:" times="">The results of the experiments showed that sea cucumber extract with different dilutions had an antibacterial effect on Escherichia coli bacteria, Micrococcus luteus and Bacillus cereus. These results also applied to the tests performed with selenium nanomedicine. Interestingly, after combining a certain amount of sea cucumber extract and selenium nanomedicine, a greater antibacterial effect was observed on the above-mentioned 3 bacteria.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><b> </b><span new="" roman="" style="font-family:" times="">The synergistic effect of sea cucumber extract and selenium nanoparticles has a higher level of antibacterial properties.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> &nbsp;</div> خیار دریایی, نانوذره هیبریدی سلنیوم, آنتی‌بیوتیک Sea cucumber, Selenium hybrid nanoparticles, Antibiotics. 6655 6664 http://jssu.ssu.ac.ir/browse.php?a_code=A-10-4882-1&slc_lang=fa&sid=1 Mahnaz Mohammadi مهناز محمدی mh_mohamadi@yahoo.com 0000-0002-3376-2162 Yes Department of Biology, Islamic Azad University, Islamshahr Branch, Tehran, Iran. گروه زیست شناسی، دانشگاه آزاد اسلامی، واحد اسلامشهر، تهران، ایران. Shirin Hafezi شیرین حافظی shirinhafezi1992@gmail.com 0000-0002-3352-4284 No Department of Biotecnology, Islamic Azad University, Medicine Branch Tehran, Tehran, Iran. گروه بیوتکنولوژی، دانشگاه آزاد اسلامی، واحد علوم پزشکی تهران، تهران ایران.