fa تاثیر هشت هفته تمرینات تناوبی شنا با شدت بالا بر عوامل بیوشیمیایی متابولیسم لیپید و عملکرد حافظه هیپوکمپ موش های صحرایی نر مبتلا به استئاتوهپاتیت غیرالکلی (NASH) فیزیولوژی ورزش Exercise Physiology پژوهشي Original article <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> مکانیسم‌&shy;های مولکولی بهبود ورزش بر حافظه در هاله‌&shy;ای از ابهام است. لذا هدف از تحقیق حاضر تاثیر هشت هفته تمرینات تناوبی شنا با شدت بالا (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HIIST</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) بر عوامل بیوشیمیایی متابولیسم لیپید و عملکرد حافظه هیپوکمپ رت</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های نر مبتلا به استئاتوهپاتیت غیرالکلی (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">NASH</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) بود.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> مطالعه حاضر از نوع تجربی بود. </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">40 سر رت نر نژاد </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Sprague&ndash;Dawley</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> به دو گروه سالم (20=</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">n</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) و رژیم پرچرب </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HFD) </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">،20</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">n=</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) تقسیم شدند. رت</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های گروه </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HFD</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با هدف القا </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">NASH</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، به‌&shy;مدت هشت هفته رژیم‌&shy;غذایی پرچرب دریافت کردند. رت</span><span new="" roman="" style="font-family:" times="">‌</span><span b="" nazanin="" style="font-family:">ها بعد از کشتار 4 سر رت برای اثبات بیماری به</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">طور تصادفی به چهار گروه سالم-بی</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تحرک (9</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">n=</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">)، سالم-شنا (9</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">n=</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">)، بیماربی</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تحرک (9</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">n=</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) و بیمار-شنا (9</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">n=</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) تقسیم شدند. گروه</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های تمرینی هشت هفته </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HIIST</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> (سه جلسه/هفته، هر جلسه 30دقیقه) انجام دادند. در پایان، پروتئین </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">FNDC5</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با روش وسترن‌بلات و آیریزین هیپوکمپ مغز و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">FFA</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> سرم به&shy; شیوه الایزا ارزیابی شدند. عملکرد حافظه با آزمون رفتاری مازآبی&shy;موریس اندازه‌گیری شد. داده‌ها با استفاده از نرم‌افزار</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">SPSS version 16</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> آنالیز شد. تعیین تفاوت میان گروه</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ها از آزمون آنووای یک‌راهه و برای تعیین تفاوت دوبه&shy;دو گروه&shy;ها از آزمون تعقیبی بونفرونی استفاده شد (0/05</span></span><span lang="FA" style="font-size:12.0pt">></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">). </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> یافته</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ها نشان داد در پروتئین </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">FNDC5</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و آیریزین هیپوکمپ مغز و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">FFA</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> سرم و در متغیرهای عملکرد حافظه میان گروه</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ها تفاوت معنادار وجود داشت (0/05</span></span><span lang="FA" style="font-size:12.0pt">></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">). </span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجه‌گیری:</span></span></b> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HIIST</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در مدل </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">NASH</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">؛ می</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تواند متابولیسم لیپید در هیپوکمپ مغز را فعال کند و عملکرد حافظه را بهبود بخشد. با این</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">حال تحقیقات بیشتر در این زمینه مورد نیاز است.</span></span></span></span></span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:16.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Introduction:</span></b> <span new="" roman="" style="font-family:" times="">The molecular mechanisms of the improvement of exercise on memory are in the aura of ambiguity.</span><span new="" roman="" style="font-family:" times="">Therefore, the aim of this research was studying the effect of eight weeks of high-intensity intermittent swimming training (</span><span new="" roman="" style="font-family:" times="">HIIST)</span><span new="" roman="" style="font-family:" times=""> on the biochemical factors of lipid metabolism and hippocampal memory function of male rats with non-alcoholic steatohepatitis (NASH).</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:16.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b> <span new="" roman="" style="font-family:" times="">The present study was experimental. 40 male Sprague-Dawley rats were divided into two groups: healthy (n=20) and high-fat HFD (n=20) groups. Rats in the HFD group were fed a high-fat diet for eight weeks with the aim of inducing NASH. After proving the disease in HFD group rats, the remaining rats were randomly divided into four groups: healthy-</span><span new="" roman="" style="font-family:" times="">sedentary </span><span new="" roman="" style="font-family:" times="">(n=9), healthy-swimming (n=9), </span><span new="" roman="" style="font-family:" times="">sedentary disease </span><span new="" roman="" style="font-family:" times="">(n=9) and diseased-swimming (n=9). The exercise groups performed eight weeks of HIIST (three sessions/week, each session lasting 30 minutes). At the end, FNDC5 protein was evaluated by western blot method and brain hippocampal irisin and serum FFA by ELISA method. Memory performance was measured by the water Maz-Morris behavioral test. One-way ANOVA test was used to determine the difference between groups, and Bonferroni&#39;s post hoc test was used to determine the difference between two groups (p<0.05).</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:16.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><b> </b><span new="" roman="" style="font-family:" times="">The findings showed that there was a significant difference in the FNDC5 protein and irisin of the brain hippocampus and serum FFA and in memory function variables between the groups (p<0.05).</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:16.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><b> </b><span new="" roman="" style="font-family:" times="">HIIST in NASH model can activate lipid metabolism in the hippocampus of the brain and improve memory function. However, more research is needed in this field.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> &nbsp;</div> استئاتوهپاتیت غیرالکلی, شنای تناوبی با شدت بالا, FNDC5, هیپوکمپ, آیریزین, متابولیسم لیپید, عملکرد حافظه Nonalcoholic steatohepatitis, High-intensity interval swimming, FNDC5, Hippocampus, Irisin, Lipid metabolism, Memory function 6378 6395 http://jssu.ssu.ac.ir/browse.php?a_code=A-10-5042-1&slc_lang=fa&sid=1 Bahare Zare بهاره زارع bahare.zare5@gmail.com 0000-0002-3346-6224 No Department of Sports Physiology, Faculty of Educational Sciences and Psychology, Department of Sports Sciences, Shiraz University, Shiraz, Iran. گروه فیزیولوژی ورزشی، دانشکده علوم تربیتی و روانشناسی، گروه علوم ورزشی، دانشگاه شیراز، شیراز، ایران. Farhad Daryanoosh فرهاد دریانوش daryanoosh@shirazu.ac.ir 0000-0003-2125-896X Yes Department of Sports Physiology, Faculty of Educational Sciences and Psychology, Department of Sports Sciences, Shiraz University, Shiraz, Iran. گروه فیزیولوژی ورزشی، دانشکده علوم تربیتی و روانشناسی، گروه علوم ورزشی، دانشگاه شیراز، شیراز، ایران. Rasoul Rezaei رسول رضایی rasoul.rezai1364@gmail.com 0000-0001-5136-3694 No Department of Sports Sciences, Faculty of Educational Sciences and Psychology, Shiraz University, Iran. بخش علوم ورزشی، دانشکده علوم تربیتی و روانشناسی، دانشگاه شیراز، شیراز، ایران. Javad Nemati جواد نعمتی Jnemati@shirazu.ac.ir 0000-0002-6925-1727 No Department of Sports Sciences, Faculty of Educational Sciences and Psychology, Shiraz University, Iran. بخش علوم ورزشی، دانشکده علوم تربیتی و روانشناسی، دانشگاه شیراز، شیراز، ایران. Mohammad Hemmatinafar محمد همتی نفر m.hemmatinafar@shirazu.ac.ir 0000-0001-7585-7592 No Department of Sports Sciences, Faculty of Educational Sciences and Psychology, Shiraz University, Iran. بخش علوم ورزشی، دانشکده علوم تربیتی و روانشناسی، دانشگاه شیراز، شیراز، ایران.