The Journal of Shahid Sadoughi University of Medical Sciences
مجله علمي پژوهشي دانشگاه علوم پزشكي شهید صدوقی يزد
JSSU
Medical Sciences
http://jssu.ssu.ac.ir
20
journal20
2228-5741
2228-5733
fa
jalali
1402
5
1
gregorian
2023
8
1
31
5
online
1
fulltext
fa
بررسی اثر ضد میکروبی نانو ذرات نقره و ترکیب با کوتریموکسازول علیه سالمونلا تیفی در شرایط آزمایشگاهی و مدل حیوانی
Antimicrobial Effect of Silver Nanoparticles and Combination with Cotrimoxazole against Salmonella Typhi in Vitro and in Animal Model
میکروبیولوژی
Microbiology
پژوهشي
Original article
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سالمونلا</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> تیفی دسته بزرگی از باسیلهای گرم منفی با خصوصیت انتروباکتریاسه</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و بیماریزای مشترک بین انسان و حیوان است که عامل اصلی بیماری حصبه در انسان میباشد. یکی از شایعترین آنتیبیوتیکها در درمان سالمونلا، کوتریموکسازول میباشد ولی به دلیل استفاده گسترده از آنتیبیوتیکها، مقاومت باکتریایی، به وجود آمده است. هدف از تحقیق حاضر بررسی اثر ضد باکتریایی نانو ذرات نقره و ترکیب آن با آنتیبیوتیک کوتریموکسازول به منظور تولید یک داروی ضد میکروبی مؤثرتر علیه سالمونلا تیفی بود.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در این مطالعه</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تجربی</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، حداقل غلظت مهارکننده رشد (</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">MIC</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) و حداقل غلظت کشنده باکتری (</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">MBC</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) برای </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نانو ذرات نقره و ترکیب با کوتریموکسازول </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بر اساس روش مایکرودیلوشن انجام شد. سپس </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">اثر ضد میکروبی آنها در مدل موش آلوده بررسی شد. </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">به منظور تجزیه و تحلیل آماری نتایج حاصل، </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">version 16</span> <span dir="LTR" new="" roman="" style="font-family:" times="">SPSS</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> مورداستفاده قرار گرفت. در این آزمون 0/05 </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">P<</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> به عنوان سطح معنیداری در نظر گرفته شد.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b> <span dir="LTR" new="" roman="" style="font-family:" times="">MIC</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">MBC</span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> نانو ذرات نقره برای </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سالمونلا تیفی </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">به ترتیب </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">ppm</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> 4 و </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">ppm</span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">8</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، ترکیب نانو ذرات نقره با کوتریموکسازول</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> برای </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سالمونلا تیفی </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">ppm</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> 62 و</span></span> <span dir="LTR" new="" roman="" style="font-family:" times="">ppm</span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">125 و کوتریموکسازول </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">برای </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سالمونلا تیفی</span></span> <span dir="LTR" new="" roman="" style="font-family:" times="">ppm</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> 500 و </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">ppm</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> 1000 بو</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">دند. مدل موشی برای بررسی اثر ضد میکروبی </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نانو ذرات نقره </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">علیه عفونت </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سالمونلا تیفی </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تائید شد. به طوریکه </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نانو ذرات نقره بیشترین اثر ضد میکروبی و کوتریموکسازول</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">کمترین اثر ضد میکروبی نسبت به سایر گروهها داشتند.</span></span> <span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجهگیری:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نانو ذرات نقره در مقایسه با سایر گروهها به ویژه کوتریموکسازول آنتیباکتریایی بسیار مؤثری است</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">.</span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br>
</div>
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Introduction:</span></b> <i><span new="" roman="" style="font-family:" times="">Salmonella typhi</span></i><span new="" roman="" style="font-family:" times=""> is a large group of gram-negative bacilli with Enterobacteriaceae and common pathogens between humans and animals, which is the main cause of typhoid fever in humans. One of the most common antibiotics in the treatment of Salmonella is cotrimoxazole, but due to the widespread use of antibiotics, bacterial resistance has developed. The aim of this study was to investigate the antibacterial effect of silver nanoparticles and its combination with the antibiotic cotrimoxazole to produce a more effective antimicrobial drug against <i>Salmonell typhi</i>.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b> <span new="" roman="" style="font-family:" times="">In this</span><span new="" roman="" style="font-family:" times=""> experimental</span><span new="" roman="" style="font-family:" times=""> study, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for silver nanoparticles and combination with cotrimoxazole, was performed based on microdilution method. Then, their antibacterial effects were investigated in infected mouse model.</span> <span new="" roman="" style="font-family:" times="">In order to analyze the results statistically, SPSS software version 16 was used. In this test, P< 0.05 was considered as a significant level.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><b> </b><span new="" roman="" style="font-family:" times="">MIC and MFC of silver nanoparticles for <i>Salmonella typhi</i> were 4 ppm and 8 ppm respectively, composition of silver nanoparticles with cotrimoxazole for <i>Salmonella</i> <i>typhi</i> were 62 ppm and 125 ppm and cotrimoxazole for <i>Salmonella</i> <i>typhi</i> were 500 ppm and 1000 ppm. Mouse model to study the antibacterial effect of silver nanoparticles and combination with cotrimoxazole against <i>Salmonella</i> <i>typhi</i> infection was confirmed., Silver nanoparticles had the highest antibacterial effect and cotrimoxazole has the lowest antibacterial effect than other groups.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><b> </b><span new="" roman="" style="font-family:" times="">Silver nanoparticles are very effective compared to other groups, especially the antibacterial cotrimoxazole.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br>
</div>
سالمونلا تیفی, نانو ذرات نقره, کوتریموکسازول, اثر ضد میکروبی
Salmonella typhi, Silver nanoparticles, Cotrimoxazole, Antimicrobial effect.
6675
6682
http://jssu.ssu.ac.ir/browse.php?a_code=A-10-4969-1&slc_lang=fa&sid=1
Mina
Javadi
مینا
جوادی
mina.javadi6186@gmail.com