fa بررسی اثرات ضد افسردگی عصاره الکلی مرزنجوش وحشی (Origanum Vulgare L) در موش های سوری نر بالغ تحت مسمومیت با رزرپین فیزیولوژی Physiology پژوهشي Original article <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مرزنجوش وحشی گیاهی غنی از ترکیبات آنتی&shy;اکسیدان بوده و قابلیت درمانی دارد. هدف از این تحقیق، بررسی اثرات عصاره الکلی مرزنجوش وحشی بر عوارض افسردگی در موش&shy;های سوری تحت مسمومیت با رزرپین بود.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در این مطالعه تجربی، 42 موش سوری‌نر </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">BALB/c</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> به 6 گروه 7 تایی شامل کنترل (نرمال سالین)، کنترل منفی (رزرپین)، کنترل مثبت (رزرپین+فلوکستین) و سه گروه رزرپینی تحت</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">درمان با عصاره مرزنجوش به</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ترتیب با دوزهای 100، 200 و 400 میلی</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">گرم بر کیلوگرم از راه درون صفاقی طبقه</span><span new="" roman="" style="font-family:" times="">‌</span><span b="" nazanin="" style="font-family:">بندی شدند. جهت ایجاد افسردگی از مدل شنای اجباری استفاده شد. همین</span><span new="" roman="" style="font-family:" times="">‌</span><span b="" nazanin="" style="font-family:">طور ظرفیت آنتی</span><span new="" roman="" style="font-family:" times="">‌</span><span b="" nazanin="" style="font-family:">اکسیدانی و سطح مالون</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">دی</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">آلدئید سرم و مغز اندازه</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">گیری شد. داده</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ها توسط نرم</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">افزار</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">version 16 </span></span>&nbsp;<span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">SPSS</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و آنالیز واریانس یک</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span><span b="" nazanin="" style="font-family:">طرفه در سطح معناداری 0/05</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&ge;</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> تجزیه و تحلیل شدند.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج نشان‌دهنده افزایش مدت</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">زمان بی</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">حرکتی و کاهش مدت</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">زمان شناکردن در گروه کنترل منفی در مقایسه با گروه کنترل شد (0/001</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&ge;</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) و عصاره مرزنجوش وحشی در سطوح 200 و 400 میلی</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">گرم برکیلوگرم مشابه فلوکستین مدت</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">زمان بی</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">حرکتی را کاهش و مدت</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">زمان شناکردن را افزایش داد (0/001</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&ge;</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">). رزرپین در گروه کنترل منفی موجب کاهش ظرفیت آنتی</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">اکسیدان و افزایش سطح مالون</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">دی</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">آلدئید سرم و مغز در مقایسه با گروه کنترل شد (0/001</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&ge;</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">). در مقایسه با گروه کنترل منفی، عصاره مذکور در سطح 200 میلی</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">گرم برکیلوگرم مشابه فلوکستین، ظرفیت آنتی</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">اکسیدانی در مغز را افزایش (0/01</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&ge;</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) و سطح مالون-دی</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">آلدئید در سرم و مغز را کاهش (0/001</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&ge;</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) داد. همینطور عصاره مرزنجوش وحشی در سطح 400 میلی</span><span new="" roman="" style="font-family:" times="">‌</span><span b="" nazanin="" style="font-family:">گرم برکیلوگرم، ظرفیت آنتی</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">اکسیدانی را افزایش و سطح مالون</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">دی</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">آلدئید در سرم و مغز را کاهش داد (0/001</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&ge;</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">).</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجه‌گیری:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">به</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نظر می</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">رسد مرزنجوش وحشی به واسطه داشتن ترکیبات آنتی</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">اکسیدان فعال، پتانسیل قابل‌توجهی در کاهش علائم افسردگی و کاهش استرس اکسیداتیو دارد.</span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Introduction:</span></b> <span new="" roman="" style="font-family:" times="">Origanum Vulgare (Oregano) is a plant rich in antioxidant compounds and has therapeutic effects. The aim of this study was to investigate the effects of Oregano alcoholic extract on complications of depression in reserpine-poisoned mice.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:">&nbsp;<b><span new="" roman="" style="font-family:" times="">Methods:</span></b> <span new="" roman="" style="font-family:" times="">In this experimental study, 42 male BALB/c mice were divided into 6 groups of 7, including control (normal saline), negative control (reserpine), fluoxetine (positive control), and three reserpine groups treated respectively with 100, 200 and 400 mg/kg of Oregano via the intraperitoneal route. Depression was evaluated using forced swim test. Brain and serum total antioxidant capacity and malondialdehyde level were also determined. The data were analyzed by SPSS statistical software and One-Way ANOVA test. The significant was shown with (p</span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&ge;</span></span><span new="" roman="" style="font-family:" times="">0.05).</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><b> </b><span new="" roman="" style="font-family:" times="">Negative control group significantly increased immobility time and decreased swimming time compared to the control group in the forced swim test (p</span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&ge;</span></span><span new="" roman="" style="font-family:" times="">0.001) and the extract at levels of 200 and 400 mg/kg similar to fluoxetine significantly reduced the immobility and increased the swimming time (p</span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&ge;</span></span><span new="" roman="" style="font-family:" times="">0.001). Reserpine in the negative control group decreased the antioxidant capacity and increased the level of malondialdehyde in serum and brain compared to the control group (p&ge;0.001).Compared to the negative control group, the extract at the level of 200 mg/kg, similar to fluoxetine, increased the antioxidant capacity in the brain (p&ge;0.01) and decreased the level of malondialdehyde in the serum and brain (p&ge;0.001). In addition, the extract at level of 400 mg/kg similar to fluoxetine significantly increased the total antioxidant capacity and decreased the level of malondialdehyde in serum and brain (p</span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&ge;</span></span><span new="" roman="" style="font-family:" times="">0.001) compared to control group.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><b> </b><span new="" roman="" style="font-family:" times="">Oregano seems to have significant potential in reducing depressive symptoms and reducing oxidative stress due to its active antioxidant compounds.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> &nbsp;</div> مرزنجوش وحشی, رزرپین, افسردگی, موش سوری نر, استرس اکسیداتیو. Oregano, Reserpine, Depression, Male mice, Oxidative stress. 6428 6439 http://jssu.ssu.ac.ir/browse.php?a_code=A-10-179-4&slc_lang=fa&sid=1 Rahmatollah Parandin رحمت‌الله پرندین rparandin@gmail.com 0000-0002-2255-5360 Yes Department of Biology, Payame Noor University, Tehran, Iran. گروه زیست شناسی، دانشگاه پیام نور، تهران، ایران. Faeze Abbasi فائزه عباسی faezeabasi283@gmail.com 0000-0003-3845-1002 No Department of Biology, Payame Noor University, Tehran, Iran. گروه زیست شناسی، دانشگاه پیام نور، تهران، ایران.