fa تأثیر 4 هفته پیش آماده سازی با تمرین تناوبی با شدت بالا و تداومی با شدت متوسط بر مقادیر TNF-α و TLR4 مغز رت‌های صحرایی نر نژاد ویستار فیزیولوژی ورزش Exercise Physiology پژوهشي Original article <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">التهاب در مغز با نام التهاب عصبی شناخته می‌شود و موجب افزایش ترشح </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">TNF-&alpha;</span> <span new="" roman="" style="font-family:" times="">(</span><span dir="LTR" new="" roman="" style="font-family:" times="">Tumor necrosis factor alpha</span><span new="" roman="" style="font-family:" times="">)</span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و فعال‌سازی مسیر </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">TLR4</span> <span new="" roman="" style="font-family:" times="">(</span><span dir="LTR" new="" roman="" style="font-family:" times="">Toll-like receptor 4</span><span new="" roman="" style="font-family:" times="">)</span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">می&shy;‌گردد. پژوهش حاضر با هدف تأثیر 4 هفته پیش آماده‌&shy;سازی با تمرین تناوبی با شدت بالا (</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">HIIT</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) و تداومی با شدت متوسط (</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">MICT</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) بر مقادیر </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">TNF-&alpha;</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">TLR4</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> رت&shy;‌های صحرایی نر نژاد ویستار انجام شد.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">پژوهش حاضر از نظر هدف کاربردی و از نظر روش تحقیق ‌تجربی بود.</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تعداد 18 سر رت نر ویستار در سه گروهِ تمرین </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">HIIT</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، تمرین </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">MICT</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و گروه کنترل تقسیم شدند. برنامه </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">HIIT</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> شامل 6 وهله فعالیت 2 دقیقه&shy;ای با شدت معادل 90-85 درصد </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">VO2max</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و 5 وهله استراحت فعال 2 دقیقه&shy;ای و برنامه </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">MICT</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با شدت معادل 65 درصد </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">VO2max</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با مدت زمان همسان با </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">HIIT</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> به مدت 4 هفته و 5 جلسه در هفته اجرا گردید. 48 ساعت بعد از آخرین جلسه تمرینی، موش&shy;‌های هر سه گروه تشریح شدند و بافت‌های قشر و استراتوم مغز موش‌&shy;ها جهت سنجش&nbsp; </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">TNF-&alpha;</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">TLR4</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با استفاده از روش وسترن بلات، استخراج شد. تحلیل داده‌&shy;ها با استفاده از آزمون آنالیز واریانس یک&shy;‌راهه (</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">ANOVA</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) در سطح معناداری 0/05 </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">به کمک نرم‌افزار</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">version 16 </span>&nbsp;<span dir="LTR" new="" roman="" style="font-family:" times="">SPSS</span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">انجام شد.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج پژوهش حاضر نشان داد متغیرهای </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">TNF-&alpha;</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">TLR4</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در نواحی قشر و استراتوم مغزی پس از 4 هفته تمرین هوازی تناوبی پر شدت و تداومی با شدت متوسط تغییر معناداری نسبت به گروه کنترل نداشتند (0/05</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">p&ge;</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">).</span></span> <span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجه‌گیری:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">با توجه به این یافته</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ها می</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">توان اظهار داشت که پیش</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">آماده</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سازی با تمرین تناوبی با شدت بالا و تمرین تداومی با شدت متوسط اثر معناداری بر شاخص</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های التهابی در مغز ندارد.</span></span></span></span></span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Introduction:</span></b> <span new="" roman="" style="font-family:" times="">Inflammation in the brain is known as neuroinflammation and increases TNF-&alpha; (</span><span new="" roman="" style="font-family:" times="">Tumor necrosis factor alpha</span><span new="" roman="" style="font-family:" times="">) secretion and activates the TLR4 (</span><span new="" roman="" style="font-family:" times="">Toll-like receptor 4) </span><span new="" roman="" style="font-family:" times="">pathway. The aim of this study was to evaluate the effect of 4 weeks preconditioning with high intensity intermittent training (HIIT) and moderate intensity training (MICT) on TNF-&alpha; and TLR4 levels in male Wistar rats.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b><span new="" roman="" style="font-family:" times=""> The current research was an experimental study.</span><span new="" roman="" style="font-family:" times="">18 male Wistar rats were divided into three groups: high intensity intermittent training (6 heads), continuous moderate intensity training (6 heads) and the control group (6 heads). HIIT program included 6 stages of 2-minute activity with intensity of 85-90% VO2max and 5 stages of active rest for 2 minutes and MICT program with intensity of 65% VO2max with the same duration as HIIT for 4 weeks and 5 days per week was implemented. 48 hours after the last training session, rats were dissected and cortical tissues and layers of mice were extracted for TNF-&alpha; and TLR-4 using western blotting. Data analysis was performed using ANOVA test at a significance level of 0.05 With the help of SPSS version 16 software.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><b> </b><span new="" roman="" style="font-family:" times="">The results of the present study showed that TNF-&alpha; and TLR4 in the cortex and stratum cerebral areas after 4 weeks of high-intensity and moderate-intensity intermittent aerobic exercise did not change significantly compared to the control group (p&ge;0.05).</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><b> </b><span new="" roman="" style="font-family:" times="">According to these findings, it can be stated that exercise with high or moderate intensity cannot be an effective factor in increasing inflammatory markers in the brain.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> &nbsp;</div> تمرین تناوبی با شدت بالا, تمرین تداومی با شدت متوسط, پیش آماده سازی, TNF-α, TLR4 HIIT, MICT, Preconditioning, TNF-α, TLR4. 6000 6012 http://jssu.ssu.ac.ir/browse.php?a_code=A-10-4859-1&slc_lang=fa&sid=1 Abbas Foroughi Pordanjani عباس فروغی پردنجانی Abbasforoughi233@gmail.com 0000-0003-3642-3745 No Department of Sports Science, Faculty of Educational Sciences and Psychology, Shiraz University, Shiraz, Iran. بخش علوم ورزشی، دانشکده علوم تربیتی و روان شناسی، دانشگاه شیراز، شیراز، ایران. Mohsen Salesi محسن ثالثی mhsnsls@gmail.com 0000-0003-2969-9102 Yes Department of Sports Science, Faculty of Educational Sciences and Psychology, Shiraz University, Shiraz, Iran. بخش علوم ورزشی، دانشکده علوم تربیتی و روان شناسی، دانشگاه شیراز، شیراز، ایران. Rasoul Rezaei رسول رضایی rasoul.rezai1364@gmail.com 0000-0001-5136-3694 No Department of Sports Science, Faculty of Educational Sciences and Psychology, Shiraz University, Shiraz, Iran. بخش علوم ورزشی، دانشکده علوم تربیتی و روان شناسی، دانشگاه شیراز، شیراز، ایران. Javad Nemati جواد نعمتی Nemati_phy@yahoo.com 0000-0000-6925-1727 No Department of Sports Science, Faculty of Educational Sciences and Psychology, Shiraz University, Shiraz, Iran. بخش علوم ورزشی، دانشکده علوم تربیتی و روان شناسی، دانشگاه شیراز، شیراز، ایران.