The Journal of Shahid Sadoughi University of Medical Sciences
مجله علمي پژوهشي دانشگاه علوم پزشكي شهید صدوقی يزد
JSSU
Medical Sciences
http://jssu.ssu.ac.ir
20
journal20
2228-5741
2228-5733
fa
jalali
1401
7
1
gregorian
2022
10
1
30
7
online
1
fulltext
fa
اثر شدت تمرین بر بیان ژن HIF-1aو محتوای تریگلیسیرید قلبی رتهای مبتلا به بیماری کبد چرب غیر الکلی
Effect of Training Intensity on HIF-1a Gene Expression and Cardiac TG Content in Rats with Non-Alcoholic Fatty Liver Disease
فیزیولوژی ورزش
Exercise Physiology
پژوهشي
Original article
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b> <i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HIF-1a</span></span></i> <span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> یک تنظیمکننده مهم در پاسخ به هیپوکسی است که بر روی ژنهای مختلف درگیر در مسیر متابولیسم انرژی تاثیر میگذارد</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> .</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">هدف این پژوهش بررسی تاثیر شدت تمرین بر بیان ژن </span></span> <i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HIF-1a</span></span></i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و محتوای تریگلیسیرید قلبی رتهای مبتلا به بیماری کبد چرب غیر الکلی بود.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> <span style="color:black">مطالعه حاضر به صورت تجربی بر روی40 </span>سر<span style="color:black"> موش صحرایی نر نژاد ویستار انجام شد. موشها به صورت تصادفی به 4 گروه تقسیم شدند. گروههای کنترل، تمرین استقامتی کم شدت (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">LIET</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">) و تمرین اینتروال شدید (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">HIIT</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">) که به مدت 16 هفته غذای پرچرب مصرف کردند و سپس دو گروه تمرینی به مدت 8 هفته در تمرینات ورزشی شرکت نمودند. همچنین گروه شم که در این مدت از غذای استاندارد استفاده نمود. در پایان میزان بیان </span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HIF-1a</span></span></i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> و میزان چربی درون بافتی قلب 4 گروه اندازهگیری شد. </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سنج<span style="color:black">ش بیان</span></span></span> <i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">HIF-1a</span></span></span></i> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">با استفاده از تکنیک </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Real-time PCR</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و سنجش </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">TG</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> روی دستگاه اتو آنالیزور انجام شد. برای تجزیه و تحلیل دادهها از نرمافزار</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">SPSS version 16</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و آزمون تحلیل واریانس استفاده شد.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> نتایج پژوهش نشان داد تفاوت معنیداری در بیان ژن </span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HIF-1a</span></span></i><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> بین گروه کنترل با گروههای شم ،</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HIIT</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">LIET</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> (0/001=</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) وجود دارد. همچنین تفاوت معنیداری در میزان </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">TG</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">(</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Triglycride</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) بین گروه</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">­</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HIIT</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">LIET</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در مقایسه با گروه شم و گروه کنترل مشاهده شد، اما بین گروه</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">­</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HIIT</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">LIET</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> تفاوت معنی­داری مشاهده نشد (0/0001=</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">). </span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجهگیری:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> نتایج نشان داد تمرینات تناوبی شدید و تمرینات تداومی کم شدت احتمالاً از طریق کاهش بیان </span></span> <i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HIF-1a</span></span></i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و افزایش اکسیداسیون اسیدهای چرب باعث کاهش محتوای </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> TG</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بافت قلبی میشود.</span></span></span></span></span></span></span></div>
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Introduction:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> HIF-1a is an important regulator in response to hypoxia that affects various genes involved in energy metabolism. The aim of this study was to investigate the effect of exercise intensity on <i>HIF-1a</i> gene expression and cardiac TG content in rats with non-alcoholic fatty liver disease.</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Methods:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> The present study was performed experimentally on 40 male Wistar rats. Mice were randomly divided into 4 groups. The control groups, low-intensity endurance training and intense interval training, consumed high-fat food for 16 weeks, and then the two training groups participated in exercise for 8 weeks. Furthermore, sham group, which used standard food during this period, at the end of training, the expression level of <i>HIF-1a</i> and the amount of intra-tissue fat of the heart were measured in 4 groups. <i>HIF-1a</i> expression was assessed using real-time PCR and TG assay on an auto-analyzer. SPSS software version 16 and analysis of variance were used to analyze the data.</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Results: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">The results showed that there was a significant difference in the expression of <i>HIF-1a</i> gene between the control and the sham groups, HIIT and LIET groups (P=0.001). Moreover, there was a significant difference in triglyceride between HIIT and LIET groups compared to the sham and control groups, but no significant difference was observed between HIIT and LIET groups (P=0.0001).</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Conclusion: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">The results showed that intense intermittent exercise and low-intensity continuous exercise may reduce cardiac triglyceride content by decreasing <i>HIF-1a</i> expression and increasing fatty acid oxidation.</span></span></span></span></span></span><br>
</div>
تمرین استقامتی کم شدت, تمرین اینتروال شدید, عامل قابل القا هیپوکسی – 1 آلفا, تریگلیسیرید, بافت قلبی
Low intensity endurance Training, High intensity interval training, HIF-1a, Heart tissue, Triglyceride.
5062
5076
http://jssu.ssu.ac.ir/browse.php?a_code=A-10-4858-1&slc_lang=fa&sid=1
Mahdi
Bagherian
مهدی
باقریان
bagherian_2009@yahoo.com