fa بررسی تغییرات مورفولوژیک تارهای عضلانی و پایانه‌های عصبی نورون حرکتی موش‌های صحرایی ‌نر از کودکی تا پیری فیزیولوژی Physiology پژوهشي Original article <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">هدف از پژوهش حاضر بررسی تاثیر گذر زمان و سن بر تغییرات مورفولوژیک موش‌های صحرایی نر بود.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">برای انجام این مطالعه تجربی ، 15 سر موش صحرایی‌نر نژاد ویستار در سه رده سنی 2 هفته‌ای (150 </span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&ndash;</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> 100 گرم)، 6 هفته‌ای (250 </span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&ndash;</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> 220 گرم) و 96 هفته‌ای (‌320 </span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&ndash;</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> 280 گرم) به مدت شش هفته در آزمایشگاه بافت و ژن پاسارگاد تهران نگهداری شدند و 48 ساعت پس از آن قربانی و عضله نعلی استخراج گردید. اندازه‌گیری سطح مقطع و تعداد تارهای عضلانی توسط میکروسکوپ نوری انجام گرفت. به منظور اندازه‌گیری پروتئین‌های </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">sema3a</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">NCAM</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> تارهای عضلانی،<span style="color:black"> از روش </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ایمونوهیستوشیمیایی استفاده شد. </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">برای تعیین تفاوت بین گروه‌ها از تحلیل واریانس یک طرفه با آزمون تعقیبی توکی در سطح معنی‌داری 0/05</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&ge;</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">p</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> استفاده شد. تجزیه و تحلیل آماری توسط نرم افزار </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">Prism5</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> انجام شد.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج نشان داد که تفاوت معنی‌دار در سطح مقطع تارهای عضلانی بین رده سنی کودک و جوان (0/01=</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">p</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">)</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، کودک و سالمند (0/049=</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">p</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و جوان و سالمند (0/049=</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">p</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">)</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> وجود دارد<span style="color:black">. هم‌چنین کاهش معنی‌دار در تعداد تارهای عضلانی در رده سنی سالمند نسبت به کودک </span>(0/012=</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">p</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">و سالمند به جوان </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">(0/001=</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">p</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">وجود داشت. و بیان پروتئین </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">sema3a</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> با گذر زمان بین رده های سنی کودک و جوان </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">(0/048=</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">p</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">)، کودک و سالمند </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">(0/0001=</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">P</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) و جوان و سالمند (0/0001=</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">P</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">افزایش معنی‌داری داشت. و نیز </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بیان پروتئین </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">NCAM</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با توجه به گذر زمان در سه رده سنی کودک و جوان (0/048=</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">p</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">)، کودک و کنترل (0/0001=</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">p</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) و جوان و سالمند (0/001=</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">p</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) کاهش معنی‌داری داشت.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجه‌گیری:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج نشان داد که با افزایش رشد از کودکی به جوانی هایپرتروفی افزایش و در سالمندی کاهش می‌یابد. و روند هایپرپلازی با افزایش سن کاهش خواهد داشت. و هم‌چنین پروتئین‌های درگیر در رشد آکسونی بر اثر رشد، کاهش و پروتئین‌های تخریب کننده اعصاب نیز افزایش می‌یابد.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Introduction:</span></b> <span new="" roman="" style="font-family:" times="">The aim of this study was to investigate the effect of time and age on morphological changes in male rats.</span><span style="font-family:"Times New Roman","serif""></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b> <span new="" roman="" style="font-family:" times="">For conducting this study, 15 male Wistar rats in three age groups of 2 weeks (150-100 g), 6 weeks (250-220 g) and 96 weeks (320-280 g) were kept</span> <span new="" roman="" style="font-family:" times="">in Pasargad Tissue and Gene Laboratory in Tehran City for six weeks and 48 hours later they were dissected and the soleus muscles was extracted. Cross-sectional area and number of muscle fibers were measured by light microscopy. Immunohistochemical method was used to measure sema3a and NCAM proteins of muscle fibers. To determine the differences between the groups, one-way analysis of variance with Tukey post hoc test was used at the significant level of p&le;0.05. Statistical analysis was performed by Prism5 software.</span> <span style="font-family:"Times New Roman","serif""></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><b> </b><span new="" roman="" style="font-family:" times="">The results showed that there was a significant difference in the cross section of muscle fibers between the three age groups: </span><span new="" roman="" style="font-family:" times="">children and young people (p=0.01), children and the elderly (p=0.049), and finally young people and the elderly (p=0.049). </span><span new="" roman="" style="font-family:" times="">There was also a significant decrease in the number of muscle fibers in the age group of elderly compared to children and elderly to young people (p&le;0.001). Moreover, sema3a protein expression increased significantly over time between the age groups of children and young (p&le;0.048), </span><span new="" roman="" style="font-family:" times="">children and elderly (p=0.0001) and finally young and elderly (p=0.0001)</span><span new="" roman="" style="font-family:" times="">. NCAM protein expression significantly reduced in three age groups</span><span new="" roman="" style="font-family:" times="">: children and young people (p=0.048), children and controls (p=0.0001), and young and elderly people (p=0.001).</span> <span style="font-family:"Times New Roman","serif""></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><b> </b><span new="" roman="" style="font-family:" times="">The results showed that with increasing growth from childhood to youth, hypertrophy increases and decreases in old age, in addition the process of hyperplasia will decrease with age. In addition to proteins involved in axonal growth due to growth decrease and neurodegenerative proteins also increase.</span></span></span></span><br> &nbsp;</div> هایپرتروفی, هایپرپلازی, sema3a, NCAM Hypertrophy, hyperplasia, sema3a, NCAM. 4851 4860 http://jssu.ssu.ac.ir/browse.php?a_code=A-10-4564-2&slc_lang=fa&sid=1 Mohammad Mahdi Bagherpour Tabalvandani محمد مهدی باقرپور طبالوندانی meshki_5833@yahoo.com 0000-0002-5481-1170 No Department of Physical Education and Sport Science, Faculty of Humanities, Rasht Branch, Islamic Azad University, Rasht, Iran. گروه تربیت بدنی و علوم ورزشی، دانشکده علوم انسانی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران. Mohammad Reza Fadaei Chafy محمد رضا فدائی چافی mfadaei2000@yahoo.com 0000-0003-0265-5329 Yes Department of Physical Education and Sport Science, Faculty of Humanities, Rasht Branch, Islamic Azad University, Rasht, Iran. گروه تربیت بدنی و علوم ورزشی، دانشکده علوم انسانی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران. Alireza Elmieh علیرضا علمیه elmieh1592@gmail.com 0000-0002-6266-0018 No Department of Physical Education and Sport Science, Faculty of Humanities, Rasht Branch, Islamic Azad University, Rasht, Iran. گروه تربیت بدنی و علوم ورزشی، دانشکده علوم انسانی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران.