fa تاثیر هشت هفته تمرین استقامتی در دو فاز روشنایی و تاریکی ریتم شبانه‌روزی بر شاخص استرس اکسایشی در بافت پانکراس موش‌های دیابتی فیزیولوژی ورزش Exercise Physiology پژوهشي Original article <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> هایپرگلیسمی با افزایش آسیب</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های سلولی ناشی از فشار اکسایشی در پانکراس همراه است. تاثیر تمرین ورزشی درفازهای مختلف شبانه</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">&shy;</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روزی بر محافظت پانکراس از استرس اکسایشی در شرایط دیابت ناشناخته است. هدف از پژوهش حاضر تاثیر هشت هفته تمرین استقامتی در دو فاز روشنایی و تاریکی بر شاخص استرس اکسایشی پانکراس موش</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های دیابتی بود.</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در این مطالعه، تعداد 18 سر موش </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Naval Medical Research Institute </span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&nbsp;با میانگین وزن 3/22</span></span><span lang="FA" style="font-size:12.0pt">&plusmn;</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> 26 گرم انتخاب و پس از القای دیابت از طریق غذای پرچرب و تزریق استرپتوزوتوسین (20 میلی</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">گرم برکیلوگرم)، تصادفی در 6 گروه کنترل سالم فاز روشنایی و تاریکی، کنترل دیابتی فاز روشنایی و تاریکی، تمرین استقامتی دیابتی فاز روشنایی و تاریکی قرار گرفتند. پروتکل تمرین استقامتی (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Vmax</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">50-60%)، 5 روز در هفته به مدت 8 هفته بود. پس از بی&shy;هوشی، خون نمونه&shy;ها جمع‌آوری و بافت پانکراس برداشته شد. مقاومت انسولینی، شاخص استرس اکسایشی و میزان بیان پروتئین</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Brain and Muscle ARNT-Like1 </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&nbsp;در پانکراس اندازه</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">گیری شد. آنالیز آماری با نرم‌افزار</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">version 16 </span></span>&nbsp;<span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">SPSS</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در سطح معنی‌داری 0/05 انجام شد.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> 8 هفته تمرین استقامتی موجب کاهش معنی‌دار مقاومت انسولینی (0/005=</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">)، استرس اکسایشی (0/05</span></span><span lang="FA" style="font-size:12.0pt"><span arial="" style="font-family:">˂</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) و افزایش معنی&shy;دار بیان </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Bmal1</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> (0/009=‌</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) در موش&shy;های مبتلا به دیابت شد. میانگین متغیرهای مورد بررسی در دو فاز روشنایی و تاریکی با یکدیگر اختلاف معنی</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">دار داشتند (0/05</span></span><span lang="FA" style="font-size:12.0pt"><span arial="" style="font-family:">˂</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">). </span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجه‌گیری:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تمرین استقامتی با افزایش عملکرد آنتی</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">اکسیدانی و بیان پروتئین</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های تنظیم شبانه‌روزی</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> سبب بهبود حساسیت انسولینی و آسیب اکسیدان دردیابت می</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">شود. فعالیت در فاز تاریکی سبب افزایش بیشتر متابولیسم سلول شده و انجام این نوع تمرین</span></span><span lang="FA" style="font-size:12.0pt">‌</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ها در فاز تاریکی به</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">عنوان یک استراتژی درمانی جدید به این بیماران می</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تواند مورد نظر باشد.</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:17.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Introduction:</span></b> <span new="" roman="" style="font-family:" times="">Chronic hyperglycemia is associated with an increase in cellular damage due to oxidative stress</span><span new="" roman="" style="font-family:" times=""> in pancreatic tissue</span><span new="" roman="" style="font-family:" times="">. The effect of exercise in different phases of the circadian cycle on protecting pancreatic tissue from oxidative stress in diabetic patients is unknown. The aim of this study was to investigate the effect of eight weeks of endurance training in light and dark phases of circadian rhythm on the oxidative stress index in pancreas of diabetic mice.</span> <span style="font-family:"Times New Roman","serif""></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:17.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b> <span new="" roman="" style="font-family:" times="">In this study, 18 mice from the Naval Medical Research Institute&nbsp; (26&plusmn;3.22 gr) were selected </span><span new="" roman="" style="font-family:" times="">and after inducing diabetes through high-fat food and Streptozotocin injection (20 mg/kg), </span><span new="" roman="" style="font-family:" times="">randomly divided into </span><span new="" roman="" style="font-family:" times="">6</span><span new="" roman="" style="font-family:" times=""> groups: light phase healthy control, dark phase healthy control, light phase diabetic control, dark phase diabetic control, light phase diabetic training and dark phase diabetic training. The endurance training protocol (50-60 %Vmax) was performed 5 d/w for 8 weeks. </span><span new="" roman="" style="font-family:" times="">After anesthesia, </span><span new="" roman="" style="font-family:" times="">blood samples and pancreatic tissues were removed. Insulin resistance markers, oxidative stress index and expression of Brain and Muscle ARNT-Like1 protein expression were measured in pancreas of diabetic rats. Data were analyzed by </span><span new="" roman="" style="font-family:" times="">one</span><span new="" roman="" style="font-family:" times=""> way analysis of variance at the significance level of p <0.05.</span> <span style="font-family:"Times New Roman","serif""></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:17.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><b> </b><span new="" roman="" style="font-family:" times="">The eight weeks of endurance training significantly decreased insulin resistance markers (p=0.005), oxidative stress index (p ˂0.05) and significantly increased the Bmal1 protein expression (p=0.009). </span><span new="" roman="" style="font-family:" times="">The mean values of all variables </span><span new="" roman="" style="font-family:" times="">showed significant differences between light and dark phases.</span> <span style="font-family:"Times New Roman","serif""></span></span></span></span><br> <b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span> </b><span lang="EN" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">Endurance training may improve insulin sensitivity and oxidant damage in diabetic conditions by increasing the function of the antioxidant system and the expression of </span></span><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">Circadian regulation </span></span><span lang="EN" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">proteins. Activity in the dark phase causes further increases the metabolism of cells. As a result, performing these types of exercises in the dark phase is recommended to these patients as a new treatment strategy.</span></span></div> دیابت,تمرین استقامتی, استرس اکسایشی, Bmal1 Diabetes, , Endurance Training, Oxidative Stress, Bmal1 4973 4986 http://jssu.ssu.ac.ir/browse.php?a_code=A-10-4818-1&slc_lang=fa&sid=1 Maryam Janbozorgi مریم جان بزرگی m.janbozorgy@scu.ac.ir 0000-0002-3614-727x No Department of Sport Physiology, University of Tehran, Tehran, Iran. دانشجوی دکتری، گروه فیزیولوژی ورزشی، دانشگاه تهران، تهران، ایران. Abassali Gaini عباسعلی گایینی aagaeini@ut.ac.ir 0000-0002-8679-0669 Yes Department of Sport Physiology, University of Tehran, Tehran, Iran. گروه فیزیولوژی ورزشی، دانشگاه تهران، تهران، ایران. Siroos Choobineh سیروس چوبینه Choobineh@ut.ac.ir 0000-0002-4466-0986 No Department of Sport Physiology, University of Tehran, Tehran, Iran. گروه فیزیولوژی ورزشی، دانشگاه تهران، تهران، ایران. Mohamad reza Tabandeh محمدرضا تابنده m.tabandeh@scu.ac.ir 0000-0003-3258-8550 No Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran. گروه علوم پایه، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران.