The Journal of Shahid Sadoughi University of Medical Sciences
مجله علمي پژوهشي دانشگاه علوم پزشكي شهید صدوقی يزد
JSSU
Medical Sciences
http://jssu.ssu.ac.ir
20
journal20
2228-5741
2228-5733
fa
jalali
1401
5
1
gregorian
2022
8
1
30
5
online
1
fulltext
fa
بررسی اثر تائورین و کورکومین بر لقاح داخل آزمایشگاهی در موشهای ماده نژادNMRI تحت تیمار با آکریلآمید
Effect of Taurine and Curcumin on In-Vitro Fertilization in NMRI Mice Treated with Acrylamide
سایر
other
پژوهشي
Original article
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">هدف از مطالعه حاضر بررسی اثر تائورین و کورکومین بر لقاح داخل آزمایشگاهی (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">IVF</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) در موشهای ماده تحت تیمار با آکریلآمید و همچنین تاثیر آن بر غلظت مالوندیآلدهید (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MDA</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) و ظرفیت آنتیاکسیدانی تام (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">TAC</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) میباشد.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">تعداد 48 سر موش ماده بالغ از نژاد </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d">NMRI</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d"> بهطور تصادفی در 6 گروه (کنترل، کورکومین</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d">mg/kg</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d"> 200، تائورین</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d"> mg/kg</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d"> 150، آکریلآمید</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d"> mg/kg</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">5، آکریلآمید+کورکومین </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d">mg/kg</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d"> 100و200، آکریلآمید+تائورین </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d">mg/kg</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d"> 75و 150</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d">(</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d"> قرار گرفتند. تجویز مواد بهصورت خوراکی و 35</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">روز</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">انجام شد.</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">در</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">پایان</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">میزان</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d">IVF</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">، غلظت </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d">MDA</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d"> و</span></span></span> <span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d">TAC</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d"> در</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">گروهها مورد بررسی قرار گرفت.</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">کاهش معنیداری را در تعداد اووسیت، درصد لقاح، جنینهای</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">2</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">، 4 و 8 سلولی، </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d">Compact</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d"> و بلاستوسیت در گروه آکریلآمید نسبت به کنترل نشان داد</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d"> (</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">0/05</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d">p<</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">)</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">. در گروه آکریلآمید میانگین غلظت </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d">MDA</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d"> افزایش و </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d">TAC</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d"> کاهش معنیداری را نسبت به سایر گروهها نشان داد</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d"> (</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">0/05</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d">p<</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">)</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">. افزایش معنیداری در تعداد اووسیت و درصد لقاح در گروه تائورین نسبت به کنترل مشاهده گردید </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">(</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">0/001</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d">p<</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">)</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">. افزایش معنیداری در پارامترهای فوق در گروه آکریلآمید+ تائورین با دوز </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d">mg/kg</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d"> 150 نسبت به گروه آکریلآمید مشاهده شد </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">(</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">0/05</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d">p<</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">)</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">. درصد لقاح و جنین های 2، 4 و 8 سلولی، </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d">Compact</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d"> و بلاستوسیت در گروه کورکومین کاهش معنیداری را نسبت به کنترل نشان داد </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">(</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">0/05</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d">p<</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">)</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">.</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجهگیری:</span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">نتایج بیانگر عملکرد بهتر تائورین در کاهش عوارض آکریلآمید نسبت به کورکومین میباشد. چراکه کورکومین </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">میتواند</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d"> سبب جلوگیری از </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d">IVF</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d"> شود. لذا در ابتدای بارداری باید با احتیاط بیشتری مصرف شود.</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br>
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<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Introduction:</span></b> <span new="" roman="" style="font-family:" times="">The aim of this study was to evaluate the effect of taurine and curcumin on in vitro fertilization (IVF) in female mice treated with acrylamideand and its effect on malondialdehyde (MDA) concentration and total antioxidant capacity (TAC).</span><span style="font-family:"Times New Roman","serif""></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b> <span new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d">A total of 48 adult female NMRI mice were randomly divided into 6 groups, including control, curcumin 200 mg / kg, taurine 150 mg / kg, acrylamide 5 mg / kg, acrylamide 5 mg / kg + curcumin 100 and 200, acrylamide 5 mg / kg + Taurine 75 and 150 mg / kg. </span></span><span new="" roman="" style="font-family:" times=""> The drugs were</span><span new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d"> administered orally for 35 days, and then IVF rate, MDA and TAC concentrations were examined in each group.</span></span> </span></span></span><br>
<span style="font-size:11pt"><span style="line-height:20.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><b> </b><span new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d">The results showed a significant decrease in oocyte, fertilization percentage, 2, 4 and 8 cell, Compact and blastocytes embryos in the acrylamide group compared to the control (P<0.05). In acrylamide group, MDA concentration increased and TAC showed a significant decrease compared to other groups (P<0.05). There was a significant increase in oocyte count and fertilization percentage in taurine group compared to the control (P<0.001). A significant increase in the above parameters was observed in the acrylamide + taurine group at a dose of 150 mg / kg compared to the acrylamide group (P<0.05). Percentage of fertilization and embryos of 2, 4 and 8 cells, Compact and blastocytes in the curcumin group showed a significant decrease compared to the control (P<0.05)</span></span><span new="" roman="" style="font-family:" times="">.</span><span style="font-family:"Times New Roman","serif""></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><b> </b><span new="" roman="" style="font-family:" times="">The results indicate that taurine has a better performance in reducing the side effects of acrylamide than curcumin. Since curcumin can prevent IVF, it should be used with more caution at the beginning of pregnancy.</span><span style="font-family:"Times New Roman","serif""></span></span></span></span><br>
</div>
تائورین, کورکومین, آکریلآمید, لقاح داخل آزمایشگاهی, موش
Taurine, Curcumin, Acrylamide, In Vitro Fertilization, Mice.
4828
4841
http://jssu.ssu.ac.ir/browse.php?a_code=A-10-4788-1&slc_lang=fa&sid=1
Roza
Saberi
رزا
صابری
rozasaberi64@gmail.com