The Journal of Shahid Sadoughi University of Medical Sciences
مجله علمي پژوهشي دانشگاه علوم پزشكي شهید صدوقی يزد
JSSU
Medical Sciences
http://jssu.ssu.ac.ir
20
journal20
2228-5741
2228-5733
fa
jalali
1401
7
1
gregorian
2022
10
1
30
7
online
1
fulltext
fa
بررسی ارتباط پلیمورفیسم ژن های CXCR4 و CXCL12 با استعداد ابتلا به رتینوپاتی دیابتی تکثیرشونده در بیماران دیابتی
Association of CXCL12 and CXCR4 Genes Polymorphism with Proliferative Diabetic Retinopathy in Diabetic Patients
ژنتیک
Genetics
پژوهشي
Original article
<div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بیماران مبتلا به دیابت مستعد پیشروی عوارض مرتبط با شبکیه هستند. تاکنون چندین ژن­ در ارتباط با پیشروی رتینوپاتی گزارش شده است که گروهی از این ژن­ها، ژن­های درگیر در رگزایی هستند. ژن­های </span></span><i><span dir="LTR" style="font-size:11.0pt">CXCL12</span></i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><i><span dir="LTR" style="font-size:11.0pt">CXCR4</span></i> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">از جمله مهمترین ژن­ها در این مسیر هستند که کمتر مورد مطالعه و بررسی قرار گرفته­اند.<span style="color:black"> هدف این مطالعه تعیین ارتباط میان پلیمورفیسم</span> ژن­های </span></span><i><span dir="LTR" style="font-size:11.0pt">CXCL12</span></i><i> </i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">(</span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">rs1801157</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">) </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و </span></span><i><span dir="LTR" style="font-size:11.0pt">CXCR4</span></i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> (</span></span><span dir="LTR" style="font-size:11.0pt"><span style="color:black">rs2228014</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">)</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و ریسک ابتلا به رتینوپاتی دیابتی تکثیرشونده در جمعیت یزد</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> می باشد.</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در مطالعه مورد-شاهدی حاضر پلیمورفیسم</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های </span></span>rs2228014<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span>rs1801157<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در میان 103 بیمار مبتلا به دیابت در قالب دو گروه مورد و شاهد مورد بررسی قرار گرفت. در این مطالعه، گروه شاهد شامل 49 بیمار مبتلا به دیابت بوده که عارضه رتینوپاتی را نشان نداده</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">اند و گروه مورد از 54 بیمار دیابتی دارای عارضه رتینوپاتی تکثیر شونده تشکیل شده است. تعیین ژنوتیپ پلیمورفیسم</span></span><span lang="FA" style="font-size:12.0pt"></span> rs2228014<span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با استفاده از روش </span></span>ARMS-PCR<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و واریانت </span></span>rs1801157<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> به روش </span></span>RFLP-PCR<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> صورت گرفت. به منظور تحلیل آماری داده</span></span><span lang="FA" style="font-size:12.0pt"></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ها از نرم</span></span><span lang="FA" style="font-size:12.0pt"></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">افزاز </span></span>SPSS version 16<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و آزمون کای-دو استفاده گردید.</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مدل</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های ژنتیکی مربوط به پلی</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مورفیسم</span></span><span lang="FA" style="font-size:12.0pt"></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های </span></span>rs2228014<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span>rs1801157<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در بیماران مبتلا به رتینوپاتی دیابتی تکثیرشونده در مقایسه با گروه کنترل، اختلاف معناداری از لحاظ آماری نشان نداد (0/05< </span></span>P<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">).</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجهگیری:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">این مطالعه ارتباط معناداری میان پلیمورفیسم</span></span><span lang="FA" style="font-size:12.0pt"></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های </span></span>rs2228014<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span>rs1801157<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و ریسک ابتلا به رتینوپاتی دیابتی تکثیرشونده نشان نداد. ارزیابی این پلیمورفیسم</span></span><span lang="FA" style="font-size:12.0pt"></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ها در جمعیت</span></span><span lang="FA" style="font-size:12.0pt"></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های مختلف و گسترده</span></span><span lang="FA" style="font-size:12.0pt"></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تر به منظور کسب نتایج دقیق</span></span><span lang="FA" style="font-size:12.0pt"></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تر پیشنهاد می</span></span><span lang="FA" style="font-size:12.0pt"></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">گردد.</span></span></span></span></span></span></span></span><br>
</div>
<div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:18.0pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Introduction:</b> <span style="font-size:11.0pt">Patients with diabetes are prone to develop complications related to the retina. So far, several genes have been reported in connection with the progression of retinopathy, and a group of these genes is genes involved in angiogenesis. CXCL12 and CXCR4 genes are among the most important genes in this path, which have been less studied and investigated. </span><span style="font-size:11.0pt">The aim of this study was to determine the relationship between polymorphism of CXCL12 (rs1801157) and CXCR4 (rs2228014) genes and the risk of proliferative diabetic retinopathy in the population of Yazd City.</span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:18.0pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Methods:</b> <span style="font-size:11.0pt"><span style="color:black">In the present case-control study, rs2228014 and rs1801157 polymorphisms were investigated among 103 patients with diabetes in two groups, case and control. In this study, the control group included 49 diabetic patients who did not show retinopathy complications, and the case group consisted of 54 diabetic patients with proliferative retinopathy complications. The Genotyping of rs2228014 polymorphism was examined by ARMS-PCR method and rs1801157 variant by RFLP-PCR technique. The statistical analysis of data was conducted using SPSS software version 16 and chi-square test</span></span>.</span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:18.0pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Results:</b><b> </b><span style="font-size:11.0pt"><span style="color:black">Our results represented no association among the </span></span><span style="font-size:11.0pt"><span style="color:black">rs1801157<b> </b>and rs2228014 </span></span><span style="font-size:11.0pt"><span style="color:black">polymorphisms and the risk of </span></span><span style="font-size:11.0pt"><span style="color:black">proliferative diabetic retinopathy</span></span><span style="font-size:11.0pt"><span style="color:black"> (P>0.05)</span></span>.</span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:18.0pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Conclusion:</b><b> </b><span style="font-size:11.0pt"><span style="color:black">This study did not confirm association of the rs1801157 and rs2228014 polymorphisms with </span></span><span style="font-size:11.0pt"><span style="color:black">proliferative diabetic retinopathy</span></span><span style="font-size:11.0pt"><span style="color:black"> and further studies are required to investigate the correlation of polymorphisms of interest with PDR.</span></span></span></span></span></span></span><br>
</div>
رتینوپاتی دیابتی تکثیر شونده, پلیمورفیسم, CXCL12, CXCR4
Proliferative Diabetic retinopathy, Polymorphism, CXCL12, CXCR4.
5032
5041
http://jssu.ssu.ac.ir/browse.php?a_code=A-10-3483-2&slc_lang=fa&sid=1
Saba
Gharibi
صبا
غریبی
saba.gharibi@gmail.com