The Journal of Shahid Sadoughi University of Medical Sciences
مجله علمي پژوهشي دانشگاه علوم پزشكي شهید صدوقی يزد
JSSU
Medical Sciences
http://jssu.ssu.ac.ir
20
journal20
2228-5741
2228-5733
fa
jalali
1400
12
1
gregorian
2022
3
1
29
12
online
1
fulltext
fa
بررسی سطوح سرمی سایتوکاینهای التهابی در زنان با علل مختلف ناباروری طی سیکلهای تحریک کنترل شده تخمدان
Evaluating the Serum Levels of Inflammatory Cytokines in Women with Various Causes of Infertility during Controlled Ovarian Stimulation Cycles
ایمونولوژی
Immunology
پژوهشي
Original article
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">میزان غلظت سایتوکاین­های التهابی در سطوح سرمی و نقش آنها در موفقیت سیکل­های باروری زنان دارای فاکتورهای زنانه ناباروری، ناشناخته است</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">.</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> بنابراین هدف از این مطالعه، بررسی سطوح سرمی سایتوکاین­های التهابی درزنان با علل ناباروری مختلف طی سیکل­های تحریک کنترل شده تخمدان است.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در این مطالعه مورد-شاهدی، 25 زن نابارور با علل ناباروری زنانه یا ناشناخته (گروه مورد) و 24 زن نابارور با علل ناباروری مردانه (گروه شاهد) در طی سیکلهای تحریک کنترل شده تخمدان شرکت کردند. 5 سی سی خون در روز تخمکگیری از آنها گرفته شد و پس از جداسازی سرم، میزان </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">IL6</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">TNFα</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> سرمی با روش الایزا اندازهگیری شد.</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">از نرمافزار آماری</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">version 16 </span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">SPSS</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> تست آماری </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Independent T test</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> برای مقایسه متغیرها در دو گروه استفاده شد.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در این مطالعه، در میزان </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">TNFα</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">IL6</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> بین دو گروه مورد و شاهد تفاوت معناداری یافت نش</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">د (0/05</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">).</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> همچنین تفاوت معناداری در سطح </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">TNFα</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> بر حسب علل ناباروری نیز مشاهده نشد (0/05</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">P></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">). ولی تفاوت معناداری در سطح </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">IL6</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> بر حسب انواع علل ناباروری (0/02</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">P=</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) دیده شد. تفاوت معنادار بین گروه زنان نابارور دارای تخمدان پلیکیستیک و با علل ناشناخته ناباروری با گروه زنان با فاکتور ناباروری مردانه مشاهده شد (به ترتیب با 0/008</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">P=</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و 0/02 </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">P=</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> ). همچنین بالاترین سطح </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">IL6</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> (3/36</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">±</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">28/20) مربوط به گروه ناباروری با علت ناشناخته است و ارتباط معناداری بین سطح </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">IL6</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با تعداد اووسیت و جنین یافت شد (0/01</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">P<</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">).</span></span> <span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجهگیری:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بالاتر بودن سطح سرمی</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">IL6 </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در گروه ناباروری با علت ناشناخته میتواند بهعنوان یکی از معیارهای پاتولوژی ناباروری و یک پارامتر تاثیرگذار بر تعداد اووسیت و جنین در این گروه محسوب گردد. با این وجود به مطالعات با تعداد جامعه هدف بیشتر برای تبین این شاخص احتمالی نیاز است.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></div>
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Introduction:</span></b> <span new="" roman="" style="font-family:" times="">Concentration of inflammatory cytokines in serum levels and their role in the success of reproductive cycles with female infertility factors is unknown, so the aim of this study was to evaluate the serum levels of inflammatory cytokines in women with different causes of female infertility during controlled ovarian stimulation cycles.</span><span style="font-family:"Times New Roman","serif""></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b> <span new="" roman="" style="font-family:" times="">In this case-control study, 25 infertile women with female infertility or unknown causes (case group) and 24 infertile women with male infertility causes (control group) participated in controlled ovarian stimulation cycles. 5 cc of blood was taken from them on the day of ovulation and after serum isolation, serum IL6 and TNFα levels were measured by ELISA.</span><span style="font-family:"Times New Roman","serif""></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><b> </b><span new="" roman="" style="font-family:" times="">In this study, there was no significant difference in TNFα and IL6 levels between case and control groups (P> 0.05). There was also no significant difference in TNFα levels in terms of causes of infertility (P> 0.05). However, there was a significant difference in IL6 level in terms of various causes of infertility (P = 0.02). There was a significant difference between polycystic ovarian as well as unknown group and male factor group considering serum levels of interleukin-6 (P = 0.008, P = 0.02, respectively). Furthermore, the highest level of IL6 (28.3 ±20.36) belonged to the infertility group with unknown cause and a significant relationship was found between IL6 level and the number of oocytes and embryos (P <0.01).</span><span style="font-family:"Times New Roman","serif""></span></span></span></span><br>
<b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span> </b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">Higher serum IL6 levels in the infertility group with unknown cause could be considered as a factor in the pathogenesis of infertility in the patients with unknown causes and a parameter affecting the number of oocytes and embryos in this group. However, studies with a larger target population are needed to explain this potential indicator.</span></span></div>
ناباروری, اینترلوکین-6, فاکتور نکروز دهنده تومور آلفا, سرم
Infertility, Interleukin-6, Tumor necrosis factor-α, Serum.
4373
4381
http://jssu.ssu.ac.ir/browse.php?a_code=A-10-4776-1&slc_lang=fa&sid=1
Leila
Azod
لیلا
عضد
l.azod@yahoo.com