The Journal of Shahid Sadoughi University of Medical Sciences
مجله علمي پژوهشي دانشگاه علوم پزشكي شهید صدوقی يزد
JSSU
Medical Sciences
http://jssu.ssu.ac.ir
20
journal20
2228-5741
2228-5733
fa
jalali
1402
3
1
gregorian
2023
6
1
31
3
online
1
fulltext
fa
بررسی ارتباط پلی مورفیسمهای ژن رسپتور ویتامینD با بیماران مبتلا به مالتیپل اسکلروزیس پاسخگو و غیر پاسخگو به درمان با اینترفرون بتا
Evaluation of the Relationship between Vitamin D Receptor Gene Polymorphisms and Multiple Sclerosis Patients Who Responsive or non-Responsive to Treatment with Beta Interferon
ایمونولوژی
Immunology
پژوهشي
Original article
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">هدف این مطالعه جستجوی ارتباط بین 4 پلیمورفیسم </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">FokIrs10735810 ,TaqIrs731236 ,ApaIrs797532</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">BsmIrs1544410</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> واقع در رسپتور ویتامین </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">D</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در بیماران </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MS</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> پاسخگو و غیر پاسخگو به درمان با اینترفرون بتا و هموار</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">کردن راه جهت تجویز صحیح و سریع داروی مناسب، همچنین کاهش زمان، هزینه و عدم موفقیت در درمان است.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:20.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">نوع مطالعه مشاهده­ای و روش مطالعه به صورت مورد-شاهدی است</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">.</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> از 200 بیمار </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MS</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> که تحت درمان با داروی اینترفرون بودند (100 نفر پاسخگو و 100 نفر غیر پاسخگو به درمان) نمونه خون گرفته شد و با استفاده از تکنیک </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">PCR-RFLP</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> نمونهها تحت هضم آنزیمی آنزیمهای محدودکننده </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">ApaI ,FokI ,TaqI</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">BsmI</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> قرار گرفتند. داده­ها با استفاده از نرم افزارهای </span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">SPSS version 16</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">، </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">SNP Analyzer</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> و </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">Prism</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> و آزمون­های کا </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"></span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black"></span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:102%"><span style="font-family:"B Nazanin""><span style="color:black"></span></span></span></span><span style="font-size:11.0pt"><span style="line-height:102%"><span style="font-family:"Times New Roman",serif"><span style="color:black"></span></span></span></span><span dir="RTL" style="font-size:12.0pt"><span style="line-height:102%"><span style="font-family:"B Nazanin""><span style="color:black"></span></span></span></span></span> <span style="font-size:11pt"><span style="line-height:20.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">و رگرسیون لجستیک مورد ارزیابی قرار گرفت.</span></span></span><span b="" nazanin="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:20.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> اختلاف معنیداری در فراوانیهای ژنوتیپی و آللی</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="letter-spacing:.2pt">پلیمورفیسم­های</span></span></span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:.2pt"> ApaI</span></span></span></span><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="letter-spacing:.2pt">(</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="letter-spacing:.2pt">0/68</span></span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:.2pt">p=</span></span></span><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="letter-spacing:.2pt">)، </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:.2pt">BsmI</span></span></span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> (</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="letter-spacing:.2pt">0/65</span></span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:.2pt">p=</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">)، </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:.2pt">FokI</span></span></span></span><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="letter-spacing:.2pt"> (</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="letter-spacing:.2pt">0/57</span></span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:.2pt">p=</span></span></span><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="letter-spacing:.2pt">) و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">TaqI</span></span></span></span><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt"> (</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="letter-spacing:.2pt">0/50</span></span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:.2pt">p=</span></span></span><span b="" nazanin="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.1pt">) </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در جمعیت افراد بیمار در بین دو گروه پاسخگو و غیر پاسخگو به درمان مشاهده نگردید.</span></span><b><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt"></span></span></span></span></b></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجهگیری:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> طبق مشاهدات بین پلیمورفیسمهای </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">ApaI ,FokI ,TaqI</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">BsmI</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> واقع در ژن رسپتور ویتامین </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">D</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و پاسخ یا عدم پاسخ به درمان به اینترفرون، ارتباطی وجود ندارد.</span></span></span></span></span></span></span></div>
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Introduction:</span></b> <span new="" roman="" style="font-family:" times="">Multiple Sclerosis is a chronic, inflammatory disorder of the central nervous system, which leads to demyelination of nerves. Treatment with interferon beta is one of the best approaches to reduce recurrence and progression of the disease. The aim of this study is to search for the relationship between 4 polymorphisms FokIrs10735810, TaqIrs731236, ApaIrs797532 and BsmIrs1544410 located in the vitamin D receptor in MS patients responsive and non-responsive to interferon beta treatment and pave the way for the correct and fast administration of the appropriate drug, as well as reducing time, cost and lack of success in treatment.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b><span new="" roman="" style="font-family:" times=""> The type of study is observational and the study method is case-control. Blood samples were taken from 200 MS patients who were treated with interferon (100 responders and 100 non-responders). Using PCR-RFLP technique, the samples were subjected to enzymatic digestion with restriction enzymes ApaI, FokI, TaqI and BsmI. The data were evaluated using SPSS version 16, SNP Analyzer and Prism software, and K-tests and logistic regression.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><b> </b><span new="" roman="" style="font-family:" times="">A significant difference in the genotypic and allelic frequencies of ApaI (p=0.68), BsmI (p=0.65), FokI (p=0.57) and TaqI (p=0.50) polymorphisms in the patients between the two responsive and non-responsive groups to treatment was not observed.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion</span></b><span new="" roman="" style="font-family:" times=""> According to our findings, there was no significant correlation between ApaI, FokI, TaqI and BsmI polymorphisms located in vitamin D receptor gene and response to treatment. </span><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br>
</div>
مالتیپل اسکلروزیس, رسپتور ویتامینD, اینترفرون بتا
Multiple sclerosis, Vitamin D receptor gene, Beta-interferon.
6494
6504
http://jssu.ssu.ac.ir/browse.php?a_code=A-10-4691-1&slc_lang=fa&sid=1
Zeinab
Fatahi
زینب
فتاحی
z.fatahi84@yahoo.com