The Journal of Shahid Sadoughi University of Medical Sciences
مجله علمي پژوهشي دانشگاه علوم پزشكي شهید صدوقی يزد
JSSU
Medical Sciences
http://jssu.ssu.ac.ir
20
journal20
2228-5741
2228-5733
fa
jalali
1401
2
1
gregorian
2022
5
1
30
2
online
1
fulltext
fa
تغییرات بیان ژن NLRP3 بافت پانکراس به دنبال تمرین تناوبی با شدت متوسط و شدید به همراه مکمل آلفالیپوئیک اسید لیپوزومال در موشهای دیابتی
NLRP-3 Expression in Pancreatic Tissue Following Moderate and High Intensity Interval Training with Liposomal Alpha-Lipoic Acid Supplement in Diabetic Rats
فیزیولوژی ورزش
Exercise Physiology
پژوهشي
Original article
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">فعالسازی اینفلامازوم</span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">NLRP-3 </span></i> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">از مشخصه­های التهاب مزمن در دیابت نوع دو است</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">. </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">انجام تمرینات ورزشی در کنار مصرف مکملهای آنتیاکسیدانی قادر به کنترل التهاب می­باشد</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">.</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> هدف از مطالعه حاضر، بررسی اثر شدتهای مختلف تمرین همراه با آلفالیپوئیک اسید بر بیان ژن</span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">NLRP-3</span></i> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بافت پانکراس موشها دیابتی بود.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در این مطالعه تجربی، 35 موش ویستار بهصورت تصادفی به 7 گروه: کنترل، دیابتی، دیابتی مکمل، دیابتی تمرین تناوبی شدید، دیابتی تمرین تناوبی متوسط، دیابتی تمرین تناوبی شدید+ مکمل، دیابتی تمرین تناوبی متوسط+مکمل تقسیم شدند. پروتکل تمرین تناوبی متوسط و شدید به مدت 6 هفته و 5 جلسه در هفته اجرا شد. تمرین تناوبی شدید شامل 10 وهله 4 دقیقه</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">­</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ای دویدن (با شدت 90-85% سرعت بیشینه) و تمرین تناوبی متوسط شامل 13 وهله 4 دقیقه</span><span new="" roman="" style="font-family:" times=""></span><span b="" nazanin="" style="font-family:">ای دویدن (با شدت 65-70% سرعت بیشینه) بود. آلفالیپوئیک اسید به مقدار 20 میلیگرم در هر کیلوگرم وزن در روز بهصورت گاواژ به موشها داده شد. بافت</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">­</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">برداری و خونگیری 48 ساعت بعد از آخرین جلسه تمرینی انجام شد. بیان ژن </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">NLRP-3</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> از روش </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">real-time PCR</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> اندازه</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">­</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">گیری گردید.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بیان ژن</span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">NLRP-3</span></i> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بافت پانکراس در گروه دیابتی نسبت به گروه سالم افزایش معنیداری داشت </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">(0/0001</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">P<b>=</b></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">)</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">.</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> آلفالیپوئیک اسید </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">(0/021</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">P<b>=</b></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">)</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، تمرین متوسط </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">(0/009</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">P<b>=</b></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">)</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، تمرین شدید </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">(0/001</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">P<b>=</b></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">)، تمرین متوسط+مکمل (0/003</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">P<b>=</b></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و تمرین تناوبی شدید+مکمل </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">(0/0001</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">P<b>=</b></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">)</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سطح </span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">NLRP-3</span></i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> بافت پانکراس را بهطور معنیداری نسبت به گروه دیایت کاهش دادند.</span></span> <span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجهگیری:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بیماری دیابت نوع دو با افزایش بیان ژن </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">NLRP-3</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در پانکراس همراه است و تمرین تناوبی شدید به همراه مکمل آلفالیپوئیک اسید یک مداخله موثرتر در کاهش </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">NLRP-3</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> پانکراس در دیابت در نظر گرفته میشود.</span></span></span></span></span></span></span></div>
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Introduction:</span></b> <span new="" roman="" style="font-family:" times="">Activation of NLRP-3 Inflammasome is considered an indicator of chronic inflammation in type 2 diabetes. Regular exercise training along with antioxidants supplements can control inflammation. The aim of the present study was to investigate the effect of training with different intensity and alpha lipoic acid supplement on pancreatic NLRP-3 expression in diabetic rats.</span> <span style="font-family:"Times New Roman","serif""></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b><span new="" roman="" style="font-family:" times=""> In this experimental study, 35 Wistar rats were randomly divided into seven groups: control, diabetic, diabetic+ supplement (S), diabetic+ high intensity training (HIT), diabetic+ moderate intensity training (MIT), diabetic+ high intensity training + supplementation (HIT+S), diabetic+ moderate intensity training + supplementation (MIT+S). The HIT and MIT protocols were performed five days a week for six weeks. HIIT included 10 bouts of four minutes running (at 85–90% of VO2max) and MIT, 13 bouts of four minutes running (at 65–70% of VO2max). Alpha lipoic acid was administered orally at a dose of 20 mg/kg was given to rats once a day by gavage. Blood and tissue sample was taken 24 h after last exercise session. Real-time PCR method for the relative expression of NLRP-3 gene expression was used.</span><span new="" roman="" style="font-family:" times=""> <b>Results:</b></span><b> </b><span new="" roman="" style="font-family:" times="">Expression of LRP-3 gene in pancreatic tissue was</span><span new="" roman="" style="font-family:" times=""> increased significantly in the diabetic group compared to control group (p=0.0001). In addition, S (P = 0.021), MIT (P = 0.009), HIT (P = 0.001), MIT+S (P = 0.003) and HIT+S (P = 0.0001), significantly reduced the NLRP-3 levels in pancreatic tissue compared to the diabetic group.</span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><b> </b><span new="" roman="" style="font-family:" times="">Type 2 diabetes is associated with increased mRNA NLRP-3 in pancreatic tissue. MIT and HIT plus alpha lipoic acid can be considered as an effective intervention in decreasing pancreatic mRNA NLRP-3 in diabetic rats.</span><span style="font-family:"Times New Roman","serif""></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><span style="font-family:"Times New Roman","serif""></span></span></span></span></div>
تمرین تناوبی, آلفالیپوئیک اسید, NLRP3, دیابت.
Interval training, Alpha lipoic acid, NLRP-3, Diabetes.
4593
4606
http://jssu.ssu.ac.ir/browse.php?a_code=A-10-4688-1&slc_lang=fa&sid=1
Seyed Abdollah
Fatemi
سید عبدالله
فاطمی
aminak101@yahoo.com