fa تاثیر اسانس گیاه رزماری بر بیان ژن آپوپتوتیک BIM در رده سلولی MCF-7 سرطان پستان زیست شناسی Biology پژوهشي Original article <div style="text-align: justify;"><span dir="LTR"></span><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سرطان پستان شایع</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&shy;</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ترین سرطان در بین زنان محسوب می‌شود و درمان آن با عوارض فراوانی همراه است. گیاهان دارویی نسبت به داروهای شیمیایی عوارض کمتری دارند از این جهت در درمان بسیاری از بیماری</span></span>‌<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ها اهمیت ویژه‌ای دارند. گیاه رزماری به دلیل داشتن خواص آنتی‌اکسیدانی اثرات ضد سرطانی دارد. هدف از این مطالعه ارزیابی اثر اسانس گیاه رزماری بر بیان ژن </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">BIM</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Bcl-2-like 11</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) در رده سلولی </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MCF-7</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> سرطان پستان است.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در این مطالعه تجربی، جهت ارزیابی سمیت سلولی اسانس رزماری، سلول‌های </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MCF-7</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> به مدت 24، 48 و 72 ساعت در معرض غلظت</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&not;</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های مختلفی از اسانس قرار گرفته و سپس بقای سلولی با استفاده از روش </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MTT</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> بررسی شد. سپس، سلول</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MCF-7</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> به مدت 24 ساعت در معرض اسانس قرار داده شدند تا میزان بیان ژن </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">BIM</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با استفاده از تکنیک </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Real Time PCR</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> مورد سنجش قرار گیرد. آنالیز آماری داده</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ها با استفاده از تست آماری </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">T-Student</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و نرم افزار </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">SPSS version 16</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> انجام گرفت.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> نتایج حاصل از آزمایش </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MTT</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> نشان می‌دهد که مرگ سلولی وابسته به دوز و زمان است. کمترین میزان بقا 19% پس از 72 ساعت بود و با افزایش غلظت اسانس، مرگ سلولی نیز افزایش یافت</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">(</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P<0.05</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">). نتایج </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Real Time PCR</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> نشان دهنده افزایش معنی‌داری در بیان ژن </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">BIM</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> پس از تیمار با اسانس می‌باشد (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P<0.05</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">).</span></span> <span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجه‌گیری:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج این مطالعه بیانگر اثر سایتوتوکسیک اسانس رزماری بر روی سلول</span></span><span lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&not;</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های سرطانی پستان بوده و با افزایش بیان ژن آپوپتوتیک </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">BIM</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> می‌تواند سبب القای اثرات ضد سرطانی باشد.</span></span></span></span></span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Introduction:</span></b> <span new="" roman="" style="font-family:" times="">Breast cancer is the most common cancer among women</span> <span new="" roman="" style="font-family:" times="">and its treatment is associated with many side effects. Herbal medicine has fewer side effects than chemical drugs, so they are especially important in the treatment of many diseases. Rosemary plant has anti-cancer effects due to its antioxidant properties. The aim of this study was to evaluate the effect of Rosemary essential oil on <i>BIM</i> gene expression in MCF-7 cell line.</span><span style="font-family:"Times New Roman","serif""></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b> <span new="" roman="" style="font-family:" times="">In this experimental study,</span> <span new="" roman="" style="font-family:" times="">to evaluate the cellular toxicity of rosemary essential oil, MCF-7 cells were exposed to different concentrations of essential oil for 24, 48 and 72 hours, and their survival was investigated using MTT assay. Then, MCF-7 cells were exposed to essential oil for 24 hours to determine the <i>BIM</i> gene expression using the Real Time PCR technique. Statistical analysis of data was performed using T-Student test and SPSS version 16 software.</span><span style="font-family:"Times New Roman","serif""></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">Results:</span></span></b><b> </b><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">The results of the MTT assay show that cell death is dose- and time-dependent. The effect of different amounts of essential oil on the survival rate of cancer cells was statistically significant (p˂0.05) and the lowest survival rate was 19% in 72 hours and with increasing essential oil concentration, cell death also increased. Real Time PCR results show a significant increase in BIM gene expression after essential oil treatment.</span></span><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt"></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">Conclusion:</span></span></b><b> </b><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">In conclusion, the results of this study demonstrate the cytotoxic effect of rosemary essential oil on breast cancer cells and it can increase the expression of BIM, an apoptotic gene and induce anticancer effects.</span></span><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt"></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt"></span></span></span></span></span></div> اسانس رزماری, ژن BIM, سلول MCF-7, سرطان پستان Rosemary essence, BIM gene, MCF-7 cell, Breast cancer. 4452 4461 http://jssu.ssu.ac.ir/browse.php?a_code=A-10-3360-4&slc_lang=fa&sid=1 Mozhgan Shakhseniaie مژگان شخص نیائی mozhgan.niaie@gmail.com 0000-0003-1689-7898 No Biology Department, Faculty of Sciences, Science and Arts University, Yazd, Iran. گروه زیست شناسی، دانشکده علوم، دانشگاه علم و هنر، یزد، ایران. Narges Nikoonahad Lotfabadi نرگس نیکونهاد لطف‌آبادی nikounahad_1976@yahoo.com 0000-0003-2214-7268 Yes Biology Department, Faculty of Sciences, Science and Arts University, Yazd, Iran. گروه زیست شناسی، دانشکده علوم، دانشگاه علم و هنر، یزد، ایران. Fatemah Haghirossadat فاطمه حقیرالسادات fhaghirosadat@gmail.com 0000-0002-8655-2118 No Department of Advanced Medical Sciences and Technologies, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. گروه علوم و فناوری‌های نوین، دانشکده پیراپزشکی، دانشگاه علوم پزشکی وخدمات بهداشتی و درمانی شهید صدوقی یزد، یزد، ایران.