The Journal of Shahid Sadoughi University of Medical Sciences
مجله علمي پژوهشي دانشگاه علوم پزشكي شهید صدوقی يزد
JSSU
Medical Sciences
http://jssu.ssu.ac.ir
20
journal20
2228-5741
2228-5733
fa
jalali
1400
12
1
gregorian
2022
3
1
29
12
online
1
fulltext
fa
بررسی پلیمورفیسم 4612666rs در ژن 3NLRp در بیماران دیابت نوع ۲ در آذربایجان شرقی
Study of Polymorphism Rs4612666 in NLRP3 Gene in Patients with Type 2 Diabetes in East Azerbaijan
ژنتیک
Genetics
پژوهشي
Original article
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">دیابت نوع دو، یک بیماری شایع چند فاکتوری است. مطالعات نشان داده است که ژن 3</span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">NLRP</span></i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> نقش مهمی در مقاومت به انسولین دارد. هدف از مطالعه حاضر بررسی همراهی پلیمورفیسمهای 4612666</span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">rs</span></i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> از ژن 3</span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">NLRp</span></i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با دیابت نوع دو در جمعیت استان </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">آذربایجان شرقی</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">می</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">­</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">باشد.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در این مطالعه موردی - شاهدی، نمونه خون از 110 نفر بیمار و 110 نفر سالم به عنوان گروه کنترل جمعآوری شد. به دنبال استخراج </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">DNA</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> از تمام نمونه</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">­</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ها و سنجش کیفیت با استفاده از الکتروفورز، نمونه</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">­</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ها با پرایمرهای اختصاصی خود با </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">PCR</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و الکتروفورز بررسی شدند. تعیین ژنوتیب افراد برای 4612666</span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">rs</span></i><i> </i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">با استفاده از روش </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">PCR-RFLP</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> انجام شد. </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">دادههای حاصل شده با نرم افزار</span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">version 16 </span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">SPSS</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> وسط آزمون توصیفی و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">chi-square</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> مورد بررسی قرار گرفت و سطح معنیداری کمتر از 0/05 در نظر گرفته شد.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b><i> </i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">آنالیز نتایج بین دو گروه سالم و بیمار، از نظر آماری یک اختلاف معنیداری (0/05</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">p <</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">)</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> را در فراوانی ژنوتیپ</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">­</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">TT</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">CC</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">TC</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> مربوط به پلیمورفیسم4612666</span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">rs</span></i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در موقعیت ژن 3</span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">NLRP</span></i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> نشان داد (0/000 </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">=</span> <span dir="LTR" new="" roman="" style="font-family:" times="">p</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">). درصد آلل </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">T</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> نیز در افراد سالم و بیمار به ترتیب 65/9 درصد و 45/9 درصد و درصد آلل </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">C</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در افراد سالم و بیمار به ترتیب 34/1 درصد و 54/1 درصد گزارش شد.</span></span> <span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span></span><br>
<b><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجهگیری:</span></span></b> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج در این مطالعه نشان می</span></span><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">­</span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">دهد که احتمالاً بین افزایش آلل </span></span><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">C</span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و ابتلا به دیابت می</span></span><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">­</span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تواند رابطه وجود داشته باشد اگر چه برای تایید این نتایج نیاز به انجام مطالعات گسترده می</span></span><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">­</span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">باشد. </span></span></div>
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Introduction:</span></b> <span new="" roman="" style="font-family:" times="">Type 2 diabetes is a common multifactorial disease. Studies have shown that the <i>NLRP3</i> gene plays an important role in insulin resistance. The aim of this study was to investigate the association of <i>rs4612666</i> polymorphisms of <i>NLRp3</i> gene with type 2 diabetes in the population of East Azarbaijan province.</span><span style="font-family:"Times New Roman","serif""></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b> <span new="" roman="" style="font-family:" times="">In this case-control study, blood samples from 110 patients and 110 healthy individuals as control group were collected. Following to extract of DNA from all samples and the quality assay using electrophoresis, the samples with their specific primers were examined by PCR and electrophoresis. Genotyping of individuals for <i>rs4612666</i> was performed using PCR-RFLP method</span><span new="" roman="" style="font-family:" times="">.</span> <span new="" roman="" style="font-family:" times="">Data were analyzed by SPSS software version 21 by descriptive and Chi-square test .The significance level was considered to be less than 0.05</span><span style="font-family:"Times New Roman","serif""></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><b> </b><span new="" roman="" style="font-family:" times="">Analysis of the </span><span new="" roman="" style="font-family:" times="">results showed a statistically significant difference (p <0.05) in the frequency of TT, CC and TC genotypes related to <i>rs4612666</i> polymorphism at <i>NLRP3</i> gene position (P = 0.000). The percentage of T allele in healthy and unhealthy individuals was 65.9% and 45.9%, respectively, and the percentage of C allele in healthy and unhealthy patients was 34.1% and 54.1%, respectively.</span><span style="font-family:"Times New Roman","serif""></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:18.0pt"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><b> </b><span new="" roman="" style="font-family:" times="">The results of this study suggest that there may be a link between an increase in the C allele and diabetes, although extensive studies are needed to confirm these results.</span><span style="font-family:"Times New Roman","serif""></span></span></span></span></div>
پلیمورفیسم 4612666rs, دیابت نوع 2,ژن 3NLRp
polymorphism rs4612666, Type 2 diabetes, NLRp3.
4405
4414
http://jssu.ssu.ac.ir/browse.php?a_code=A-10-4596-1&slc_lang=fa&sid=1
Fatemeh
Hosseinpour Shordarag
فاطمه
حسین پور شوردرق
fatemeh.hosseinpoor1990@gmail.com