The Journal of Shahid Sadoughi University of Medical Sciences
مجله علمي پژوهشي دانشگاه علوم پزشكي شهید صدوقی يزد
JSSU
Medical Sciences
http://jssu.ssu.ac.ir
20
journal20
2228-5741
2228-5733
fa
jalali
1400
10
1
gregorian
2022
1
1
29
10
online
1
fulltext
fa
تغییرات بیان ژن FOXE1 و lncRNA PTCSC2 در بافتهای توموری در مقایسه با بافتهای سالم در بیماران مبتلا به سرطان کولورکتال
Expression Changes of the FOXE1 and lncRNA PTCSC2 Expression in Tumor Tissues Compared with Normal Tissues in Patients with Colorectal Cancer
ژنتیک
Genetics
گزارش مورد
case report
<strong><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">مقدمه:</span></span></strong> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">در مطالعات به متیلاسیون </span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;">FOXE1</span></span></em><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> در سرطان کولورکتال پرداخته شده و از آن بهعنوان یک بیومارکر تشخیصی نام برده شده است. در این مطالعه برای اولین بار ارتباط بیانی </span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;">FOXE1</span></span></em><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> و </span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;">lncRNA PTCSC2</span></span></em> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">در سرطان کولورکتال و همچنین مقایسه الگوی بیانی آنها در دو بافت سالم و توموری افراد بیمار مورد مطالعه قرار گرفت.</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"></span></span>
<div style="text-align: justify;"><strong><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">روش بررسی:</span></span></strong> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">در این تحقیق از </span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">40 </span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">نمونه بافت بیمار مبتلا به سرطان کولورکتال</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> و 40 بافت نرمال </span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">استفاده شد</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">. سپس </span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">استخراج </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">Total RNA</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> و در ادامه سنتز </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">cDNA</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> صورت گرفت. سپس میزان رونوشت اختصاصی ژن­های </span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;">lncRNA PTCSC2</span></span></em><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> و </span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;">FOXE1</span></span></em><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> در دو بافت توموری و سالم مقایسه شد. نتایج حاصل به وسیله نرم­افزار </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">GraphPad Prism</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> مورد تحلیل آماری قرار گرفت و جهت بررسی بیان </span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;">lncRNA PTCSC2</span></span></em><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> و </span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;">FOXE1</span></span></em> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">در گروه بیماران و سالم از آزمون </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">T-test</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> و نرمافزار پریزم استفاده شد و </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">p </span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">کمتر از </span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">0/05 </span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">به عنوان اختلاف معناداری در نظر گرفته شد.</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"></span></span><br>
<strong><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">نتایج:</span></span></strong> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">سطح بیان </span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;">FOXE1</span></span></em><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> بهطور معنیداری در نمونههای توموری کاهش نشان داد (0/005=</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">P</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">) درحالیکه سطح بیان </span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;">lncRNA PTCSC2</span></span></em> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> در بافت توموری در مقایسه با بافت نرمال تغییر چشمگیری نداشت (0/65=</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">P</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">). به علاوه </span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">سطح بیان </span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;">FOXE1</span></span></em> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> و </span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;">lncRNA PTCSC2</span></span></em><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> ارتباط معناداری با پیشرفت بیماری و سن بیماران نشان نداد. </span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">نمودار </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">ROC</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> نشان داد</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> که ژن </span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;">FOXE1</span></span></em><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> میتواند به عنوان یک متغیر مستقل به صورت نسبتاً مناسبی </span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">(0/03</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">P=</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">)</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> میان دو گروه بافت سالم و بیمار مورد مطالعه تغییرات بیانی داشته باشد.</span></span> <span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;"></span></span></span><br>
<strong><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">نتیجهگیری:</span></span></strong> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">با توجه نتایج این مطالعه</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">،</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> تغییرات بیان ژن </span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;">FOXE1</span></span></em><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> در نمونه­های توموری کاهش چشمگیری داشت و در سرطان کولورکتال می­تواند به عنوان یک بیوماکر برای تشخیص تومور مورد استفاده قرار گیرد.</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"></span></span></div>
<div style="text-align: justify;"><strong>Introduction:</strong> In recent studies, methylation of <em>FOXE1</em> in colorectal cancer has been reported as a diagnostic biomarker. In this study for the first time, the expression of <em>FOXE1</em> and <em>PTCSC2</em> in colorectal cancer was investigated and their expression patterns in two healthy and tumor tissues of patients were compared.<br>
<strong>Methods:</strong> In this study, 40 tumor tissues with colorectal cancer and 40 adjacent normal samples were collected. Total RNA was extracted and cDNA synthesis followed. Then, the specific genes for <em>lncRNA PTCSC2</em> and <em>FOXE1 </em>were amplified. The results were statistically analyzed by Graph Pad Prism software and a T-test was used to compare the expression levels of <em>lncRNA PTCSC2</em> and <em>FOXE1 </em>in the patients and healthy group; p-value less than 0.05 was considered significant difference criteria.<br>
<strong>Results:</strong> In this study, <em>the</em> <em>FOXE1 </em>expression level was significantly decreased in tumor tissue (p-value = 0.005), whereas the <em>lncRNA PTCSC2</em> expression level in tumor tissue was not significantly changed (p-value = 0.65). In addition, the expression levels of <em>FOXE1 </em>and <em>lncRNA PTCSC2</em> did not show a significant relation with disease progression and age of the patients. ROC curve for changes in <em>FOXE1</em> and <em>lncRNA</em> <em>PTCSC2</em> expression showed that the<em>FOXE1</em> gene could be a relatively appropriate independent variable (p-value = 0.03) to differentiate between the two study groups.<br>
<strong>Conclusion:</strong> According to the results of this study, changes in FOXE1 gene expression were significantly reduced in tumor samples and can be used as a biomarker in tumor diagnosis in colorectal cancer.</div>
LncRNA PTCSC2, FOXE1, سرطان کولورکتال.
lncRNA PTCSC2 expression, FOXE1 expression, Colorectal Cancer.
4219
4229
http://jssu.ssu.ac.ir/browse.php?a_code=A-10-4589-1&slc_lang=fa&sid=1
Marzieh
Ghani Dehkordi
مرضیه
قانی دهکردی
marzieh.dehkordi93@gmail.com