The Journal of Shahid Sadoughi University of Medical Sciences
مجله علمي پژوهشي دانشگاه علوم پزشكي شهید صدوقی يزد
JSSU
Medical Sciences
http://jssu.ssu.ac.ir
20
journal20
2228-5741
2228-5733
fa
jalali
1400
11
1
gregorian
2022
2
1
29
11
online
1
fulltext
fa
بررسی اختلالات تعدادی در کروموزم های ۱۸،X وY در مردان نابارور الیگوتراتواسپرمی توسط تکنیک FISH
Aneuploidy Assessment of X, Y, 18 Chromosomes in Sperm of Oligoteratospermia Using FISH Technique
ژنتیک
Genetics
پژوهشي
Original article
<div style="text-align: justify;"><strong><span style="letter-spacing:-.1pt;"><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">مقدمه:</span></span></span></strong> <span style="letter-spacing:-.1pt;"><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">ناباروری،</span></span></span> <span style="letter-spacing:-.1pt;"><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">عدم باروری بعد از یک سال مقاربت بدون استفاده از وسایل جلوگیری است</span></span></span><span style="letter-spacing:-.1pt;"><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">. </span></span></span><span style="letter-spacing:-.1pt;"><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">ناباروری در گذشته عمدتاً مشکل زنانه بود اما با مشخص شدن نقش فاکتورهای مردانه، درصد قابل توجهی از ناباروریها به نواقص اسپرم اختصاص یافت.</span></span></span><span style="letter-spacing:-.1pt;"><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"></span></span></span><br>
<strong><span style="letter-spacing:-.1pt;"><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">روش بررسی</span></span></span></strong>: <span style="letter-spacing:-.1pt;"><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">این مطالعه به صورت مورد- شاهدی از نمونههای اسپرم،</span></span></span><span style="letter-spacing:-.1pt;"><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">30</span></span></span> <span style="letter-spacing:-.1pt;"><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">مرد</span></span></span> <span style="letter-spacing:-.1pt;"><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">نابارور مبتلا به الیگوتراتواسپرمی بهعنوان گروه مورد و30</span></span></span> <span style="letter-spacing:-.1pt;"><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">مرد بارور بدون سابقه ناباروری و دارای اسپرم نرمال به عنوان گروه شاهد،</span></span></span><span style="letter-spacing:-.1pt;"><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> همه افراد بیمار و شاهد در محدوده سنی 20 تا 49 سال، که برای درمان ناباروری به پژوهشکده علوم تولید مثل یزد مراجعه کرده بودند، به منظور بررسی </span></span></span><span style="letter-spacing:-.1pt;"><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">کروموزوم</span></span></span> <span style="letter-spacing:-.1pt;"><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">های 18،</span></span></span><span dir="LTR" style="letter-spacing:-.1pt;"><span style="font-family:Times New Roman,serif;"> X</span></span><span style="letter-spacing:-.1pt;"><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">و</span></span></span><span dir="LTR" style="letter-spacing:-.1pt;"><span style="font-family:Times New Roman,serif;"> Y </span></span><span style="letter-spacing:-.1pt;"><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">با استفاده از تکنیک </span></span></span><span dir="LTR" style="letter-spacing:-.1pt;"><span style="font-family:Times New Roman,serif;"> FISH</span></span><span style="letter-spacing:-.1pt;"><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">انجام گرفت. نتایج با نرمافزار</span></span></span><span dir="LTR" style="letter-spacing:-.1pt;"><span style="font-family:Times New Roman,serif;"> SPSS version 16 </span></span><span style="letter-spacing:-.1pt;"><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">و</span></span></span><span style="letter-spacing:-.1pt;"><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> تستهای</span></span></span> <span style="letter-spacing:-.1pt;"><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">آماری</span></span></span><span dir="LTR" style="letter-spacing:-.1pt;"><span style="font-family:Times New Roman,serif;"> T-TEST </span></span><span style="letter-spacing:-.1pt;"><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">و</span></span></span><span dir="LTR" style="letter-spacing:-.1pt;"><span style="font-family:Times New Roman,serif;">Chi-Square Tests </span></span> <span style="letter-spacing:-.1pt;"><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">برای تجزیه و تحلیل آماری و ضریب همبستگی </span></span></span><span dir="LTR" style="letter-spacing:-.1pt;"><span style="font-family:Times New Roman,serif;">R</span></span><span style="letter-spacing:-.1pt;"><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> پیرسون به منظور سنجش ارتباط بین متغیرها و سطح آماری 0/05 > </span></span></span><span dir="LTR" style="letter-spacing:-.1pt;"><span style="font-family:Times New Roman,serif;"> P</span></span><span style="letter-spacing:-.1pt;"><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">در نظر گرفته شد.</span></span></span><span style="letter-spacing:-.1pt;"><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"></span></span></span><br>
<strong><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">نتایج:</span></span></strong> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">اختلالات</span></span> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">مشاهده شده در</span></span> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">مردان</span></span> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">نابارور الیگوتراتواسپرمی در</span></span> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">ارتباط با گروه کنترل و بین</span></span> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">اختلالات</span></span> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">کروموزومی،</span></span> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">شمارش، مورفولوژی اسپرم (</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">01</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">/</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">0</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">></span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">P</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">) و مدت زمان ناباروری</span></span> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">(</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">01</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">/</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">0</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">></span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">P</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">) </span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">ارتباط</span></span> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">معناداری</span></span> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">وجود</span></span> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">داشت</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">. همچنین، بررسی اختلالات کروموزومی با سن افراد عدم وجود همبستگی بود ولی نرخ اختلالات کروموزومی در گروه سنی 49-40 سال به 50% افزایش یافته، که بالاترین میزان در بین گروههای سنی داشته است و قطعاً نیاز به بررسی در جامعه آماری بزرگتری دارد.</span></span> <span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;"></span></span></span><br>
<strong><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">نتیجهگیری:</span></span></strong> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">بیماران مبتلا به </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">OT</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> ممکن است در معرض خطر افزایش تولد نوزادان آنیوپلوئیدی باشند که پیشنهاد به بررسی </span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">آنیوپلوئیدی­های کروموزومی توسط تکنیک­های </span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">مولکولار سیتوژنتیک مانند </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"> FISH </span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">می­گردد</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">.</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"></span></span></div>
<div style="text-align: justify;"><strong>Introduction:</strong> Not being to get pregnant, after one year of unprotected sex is called infertility. In the post, infertility was mainly a female problem, but the role of male factors in infertility has denotation, although a greater percentage of this infertility is related to the deficiencies of semen.<br>
<strong>Methods:</strong> This case-control study was performed on sperm samples from 30 infertile oligoteratozoospermia (OT) patients as case group and 30 fertile and normosperm as controls, all the patients and controls between the ages of 20-49 years, who were referred to the Yazd Infertility Center for the treatment of infertility, were used to check the chromosomes 18, X and Y using the FISH technique. The results were considered using SPSS version 16 software and statistical tests T-TEST and Chi-Square Tests for statistical analysis and Pearson R correlation coefficient to measure the relationship between variables and statistical level P <0.05.<br>
<strong>Results:</strong> Disorders were observed in infertile oligoteratozoospermia men who were significantly related to the control group and between the chromosomal abnormalities, sperm counts and morphology <span dir="RTL">)</span>P<0/01) and there was also significant difference in correlation between chromosomal abnormalities and duration of infertility (P<0/01). In addition, there was no correlation between chromosomal abnormalities and age, but the rate of chromosomal abnormalities in the age group of 40-49 years increased to 50%, which has the highest rate among age groups and definitely needs to be examined in a larger statistical population.<br>
<strong>Conclusion:</strong> This finding suggest that patients with OT may be at an increased risk of producing aneuploid offspring. Considering the chromosomal aneuploidy is recommended by cytogenetic molecular techniques.<br>
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ناباروری مردان, اختلالات کروموزومی, الیگوتراتواسپرمی, تکنیک FISH
Male infertility, Abnormalities chromosomes, FISH.
4312
4321
http://jssu.ssu.ac.ir/browse.php?a_code=A-10-4043-1&slc_lang=fa&sid=1
Mahsa
Nasr Esfahani
مهسا
نصراصفهانی
mahsa_nasresfahani@yahoo.com