The Journal of Shahid Sadoughi University of Medical Sciences
مجله علمي پژوهشي دانشگاه علوم پزشكي شهید صدوقی يزد
JSSU
Medical Sciences
http://jssu.ssu.ac.ir
20
journal20
2228-5741
2228-5733
fa
jalali
1397
1
1
gregorian
2018
4
1
26
12
online
1
fulltext
fa
تأثیر 8 هفته تمرین استقامتی بر محتوای تام و فسفوریلاسیون پروتئینهای AKT1، mTOR، P70S6K1 و 4E-BP1 در عضله اسکلتی FHL موشهای صحرایی مبتلا به دیابت نوع 2
The effect of 8 weeks endurance exercise on the content of total and phosphorylated AKT1, mTOR, P70S6K1 and 4E-BP1 in skeletal muscle FHL of rats with type 2 diabetes
فیزیولوژی ورزش
Exercise Physiology
پژوهشي
Original article
<strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مقدمه:</span></span></strong> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مسیر </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">mTOR</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> در عضله اسکلتی نقش بسیار مهمی در فرآیند سنتز پروتئین دارد. </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">هنوز نقش فعالیت ورزشی استقامتی بر این مسیر مهم در سنتز پروتئین در افراد مبتلا به دیابت نوع 2 بررسی نشده است. هدف از مطالعه حاضر، بررسی </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">تأثیر 8 هفته تمرین استقامتی بر محتوای تام و فسفوریلاسیون پروتئینهای </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">AKT1</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">mTOR</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">P70S6K1</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> و </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">4E-BP1</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> در</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> عضله اسکلتی</span></span></span> <span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">FHL</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> موشهای صحرایی مبتلا به دیابت نوع 2 </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">میباشد.</span></span></span><br>
<strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">روش بررسی<span style="color:black;">:</span></span></span></strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> در این مطالعه</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> تجربی</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، 16 سر موش صحرایی نر از نژاد اسپراگوداولی</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> با میانگین وزن 20</span></span></span><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">±</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">270 گرم انتخاب ‏شدند و به روش تصادفی به 2 گروه کنترل (8 سر) و تمرین استقامتی (8 سر) تقسیم ‏شدند؛ گروه تمرینی 4 روز در هفته مطابق با برنامه تمرینی به‏ مدت 8 هفته به فعالیت ورزشی پرداختند؛ در حالی که گروه کنترل هیچگونه برنامه تمرینی نداشتند. برای تجزیهوتحلیل دادهها از نرم افزار 19</span></span></span> <span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">SPSS V </span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">و آزمون </span></span></span><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">t</span></span></span></span></strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">-مستقل استفاده‏ شد.</span></span></span><br>
<strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نتایج:</span></span></strong> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">تفاوت معناداری در محتوای پروتئینهای </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">AKT1</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> تام </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">(58/0</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">=</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">)، فسفریله (33/0</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">=</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">)</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">mTOR</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> تام </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">(47/0</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">=</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">)، فسفریله (78/0=</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">)</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">P70S6K1</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> تام </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">(24/0</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">=</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">)، فسفریله (12/0</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">=</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">) </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">و </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">4E-BP1</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> تام </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">(45/0</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">=</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">)، فسفریله (48/0</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">=</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">) در گروه تمرین استقامتی نسبت به کنترل مشاهده نشد.</span></span><br>
<strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نتیجهگیری:</span></span></strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> فعالیت ورزشی استقامتی به مدت 8 هفته نتوانست محتوای تام و فسفریله پروتئینهای تحقیق حاضر را افزایش دهد؛ بنابراین نمیتواند منجر به سنتز پروتئین یا هیپرتروفی عضلانی از طریق مسیر </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">mTORC1</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> شود.</span></span>
<strong>Introduction:</strong> The mTOR pathway in skeletal muscle plays a very important role in the protein synthesis process, which plays a very important role in proteins. The role of endurance exercise has not yet been studied in this important pathway in protein synthesis in people with type 2 diabetes. The purpose of the present study was to investigate the effect of 8 weeks endurance training on the content of total and phosphorylated AKT1, mTOR, P70S6K1 and 4E-BP1 in skeletal muscle FHL of rats with type 2 diabetes.<br>
<strong>Methods:</strong> In this experimental study, 16 Sprague-Dawely male rats with average weight of 270±20 were selected and randomly divided into two groups: control (n=8) and endurance training (n=8). The training group exercised according to the training program 4 days a week for 8 weeks. While the control group had no training program. <a name="OLE_LINK11">T-test and SPSS V-19 were used to analyze the data.</a><br>
<strong>Results: </strong>There was not observed any significant difference in the content of total (P=0.58) and phosphorylated (P=0.33) AKT1, total (P=0.47) and phosphorylated (P=0.78) mTOR, total (P=0.24) and phosphorylated (P=0.12) P70S6K1 and total (P=0.45) and phosphorylated (P=0.48) 4E-BP1 proteins in the endurance training group compared to the control group.<br>
<strong>Conclusion: </strong>Endurance training for 8 weeks could not increase the total and phosphorylated content proteins of the present study; therefore, it cannot lead to protein synthesis or muscle hypertrophy through mTORC1 pathway.
تمرین استقامتی, عضله اسکلتی, دیابت نوع 2
Endurance Exercise, Skeletal Muscle, Type 2 Diabete.
1063
1074
http://jssu.ssu.ac.ir/browse.php?a_code=A-10-801-11&slc_lang=fa&sid=1
Saeedeh
Shadmehri
سعیده
شادمهری
saeedeh.sh61@gmail.com