The Journal of Shahid Sadoughi University of Medical Sciences
مجله علمي پژوهشي دانشگاه علوم پزشكي شهید صدوقی يزد
JSSU
Medical Sciences
http://jssu.ssu.ac.ir
20
journal20
2228-5741
2228-5733
fa
jalali
1397
2
1
gregorian
2018
5
1
26
2
online
1
fulltext
other
مقایسۀ 6 هفته تمرین تناوبی خیلی شدید و تداومی به همراه غذای استاندارد بر بیان ژن دسنوترین و FNDC5بعد از یک دوره مصرف غذای پرچرب در دو بافت چربی زیرپوستی و عضلۀ چهار سر رتهای نر چاق
Comparing the 6 weeks of high-intensity interval and continuous training with standard diet on desnutrin and FNDC5 genes expression after a period of high fat diet in subcutaneous adipose tissue and the quadriceps muscle tissue of obese male rats
<strong><span style="color:#006386;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مقدمه</span></span></span></strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> چاقی اختلال سیستمیکی است که تنظیم پروتئینهای موثر در متابولیسم چربی را مختل میکند و به مقاومت به انسولین و دیابت منجر میشود. هدف پژوهش حاضر مقایسۀ 6 هفته تمرین تناوبی خیلی شدید و تداومی به</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">همراه غذای استاندارد بر بیان ژن <em>دسنوترین</em> و </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"> (Fibronectin type III Domain Containing protein5)</span></span></span></span> <em><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">FNDC</span></span></span></span></em><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> در دو بافت چربی زیرپوستی و عضلۀ چهار</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سر رتهای نر چاق بود.</span></span></span><br>
<strong><span style="color:#006386;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">روش بررسی:</span></span></span></strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> نوع پژوهش توسعهای و روش آن تجربی بود</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">.40 رت</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نژاد ویستار بعد از 12هفته غذای پرچرب (30</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">n=</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">) و غذای استاندارد (10</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">n=</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">) جهت اطمینان از چاقی با هم مقایسه شدند. سپس 30 رت چاق تصادفی ساده به 3 گروه تقسیم شدند: کنترل (10</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">n=</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">)، تمرین تداومی (10</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">n=</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">) و تمرین تناوبی خیلی شدید (10</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">n=</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">). تمرین تدوامی و تناوبی خیلی شدید 6 هفته و هر هفته 6 جلسه انجام شد. میزان بیان ژن <em>دسنوترین</em> و </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">FNDC5</span></span></span></span></em><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> در بافت چربی زیرپوستی و عضلۀ چهار سر به روش</span></span></span> <span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Real Time-PCR</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سنجیده شد. </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">برای مقایسۀ تغییرات بین گروهی از آزمون</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">ONE-WAY ANOVA</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> و آزمون تعقیبی توکی و برای تجزیه و تحلیل آماری از نرم</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">افزار </span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">16</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">SPSS</span></span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">و اکسل 2007 استفاده شد</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">.</span></span><br>
<strong><span style="color:#006386;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نتایج:</span></span></span></strong><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> پژوهش نشان داد بیان ژن <em>دسنوترین</em> بافت چربی زیر پوستی بین گروه تمرین تداومی و تمرین تناوبی خیلی شدید تفاوت معناداری داشت </span></span></span></span><span dir="LTR" style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">)</span></span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">011</span></span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">/0</span></span></span></span><span dir="LTR" style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">P=</span></span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">)</span></span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، ولی بیان ژن <em>دسنوترین</em></span></span></span></span><span dir="LTR" style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">)</span></span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">275</span></span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">/0</span></span></span></span><span dir="LTR" style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">P=</span></span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">) و </span></span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">FNDC5</span></span></span></span></em> <span dir="LTR" style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">)</span></span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">981</span></span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">/0</span></span></span></span><span dir="LTR" style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">P=</span></span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">)</span></span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> بافت عضله چهار</span></span></span></span> <span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سر </span></span></span></span> <span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بین دو گروه معنادار نبود</span></span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">.</span></span><br>
<strong><span style="color:#006386;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نتیجهگیری:</span></span></span></strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> به نظر میرسد برای افراد چاق و برای مقابله با آثار مخربی که چاقی بر پروتئینهای <em>دسنوترین </em>و </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">FNDC5</span></span></span></span></em><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> دارد، تمرین تداومی کارآمدتر است. ولی برای کاهش وزن، تمرین تناوبی خیلی شدید روش بهتری است.</span></span></span>
<strong>Introdution:</strong> Obesity is a systemic disorder that disruption the regulation of the effective proteins; in fat metabolism (Desnurtin, FNDC5 it leads to resistance to insulin and Diabetes. The aim of this study was comparing the 6 weeks of high-intensity interval and continuous training with standard diet on <a name="OLE_LINK72">desnutrin and FNDC5 genes expression </a>in subcutaneous adipose tissue and the quadriceps muscle tissue of obese male rats.<br>
<strong>Methods:</strong> This study was fundamental, and its method was experimental. 40 Wistar rats were compared after 12 weeks of <a name="OLE_LINK8">high-fat diet (</a>n=30) and standard diet (n=10) in order to ensure obesity. Then, 30 obese rats were divided randomly into 3 groups: control (n=10), continuous training (n=10), and high-intensity interval training groups (n=10). Continues and <a name="OLE_LINK109">high-intensity interval exercise trainings </a>were conducted according to the principle of overload for 6 weeks and 6 sessions per week. Extraction and <a name="OLE_LINK79">gene expression </a>level desnutrin in subcutaneous adipose tissue, desnutrin, and FNDC5 in quadriceps muscle tissue was assessed using real time-PCR method. The one-way ANOVA test and Tukey's post hoc test were used to compare inter group changes.<br>
<strong>Results:</strong> The study showed that the difference between <a name="OLE_LINK139">high-intensity interval and continuous training </a>on desnutrin gene expression subcutaneous <a name="OLE_LINK28">adipose tissue</a> was significant (P=0.011), but there was no significant difference in desnutrin (P=0.275) and FNDC5 genes expression (P=0.981) between them in the quadriceps muscle tissue.<br>
<strong>Conclusion:</strong> For people who suffer from obesity, and for counteracting with the destructive effects of obesity on fat metabolism factors, including desnutrin and FNDC5, continuous training can be an effective method. However, for weight loss, high intensity interval training is the best method
دسنوترین, FNDC5, تمرین تناوبی خیلی شدید, تمرین تداومی
Desnutrin, FNDC5, High-intensity interval training, Continuous training.
111
125
http://jssu.ssu.ac.ir/browse.php?a_code=A-10-3391-4&slc_lang=other&sid=1
Saeed
Rahmaty
سعید
رحمتی
s_rahmaty4290@yahoo.com