The Journal of Shahid Sadoughi University of Medical Sciences
مجله علمي پژوهشي دانشگاه علوم پزشكي شهید صدوقی يزد
JSSU
Medical Sciences
http://jssu.ssu.ac.ir
20
journal20
2228-5741
2228-5733
fa
jalali
1397
7
1
gregorian
2018
10
1
26
6
online
1
fulltext
fa
ارزیابی اثراتmiR-143 لیپوزومه بر زیست پذیری سلولی و بیان ژن آنتی آپوپتوتیک Bcl-2 در سلول های استئوسارکومای انسانی جهت اثر گذاری بردرمان سرطان های متاستاتیک
Evaluation of the effect of liposomal miR-143 on cell viability and anti-apoptotic gene expression of Bcl-2 in human osteosarcoma cells for the treatment of metastatic cancers
بیوشیمی
Biochemistry
پژوهشي
Original article
<div style="text-align: justify;"><strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مقدمه:</span></span></strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مطالعه حاضر به منظور ارزیابی اثر </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">miR-143</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> بر بیان ژن </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Bcl-2</span></span></span></span></em><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> در سطح </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">mRNA</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> و همچنین سمیت سلولی در رده سلولی</span></span></span> <span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">SaOs-2</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">(از رده های استئوسارکوما) و </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Hum- 63</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> (سلولهای سالم استخوان) انجام گرفت.</span></span></span><br>
<strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">روش بررسی:</span></span></strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در مطالعه تجربی حاضر برای انتقال </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">miR-143</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> به داخل سلول های سالم و سرطانی استخوان جهت بررسی اثرات آن بر روی بیان ژن </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Bcl-2</span></span></span></span></em><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> از لیپوزوم کاتیونی لیپوفکتامین 2000 استفاده شد سپس اثرات </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">miR-143</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> بر روی سمیت سلولی (72 ساعته) و بیان ژن </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Bcl-2</span></span></span></span></em><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> به ترتیب به وسیله تست های </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">MTT</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> و پروتکل </span></span></span> <span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">RealTimePcr</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سنجیده شد. </span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">از نرم افزار </span></span></span></span><span dir="LTR" style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">SPSS</span></span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> و آزمون های</span></span></span></span><span dir="LTR" style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">ANOVA </span></span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> و </span></span></span></span><span dir="LTR" style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:times new roman,serif;">Student t-test</span></span></span><span style="letter-spacing:-.2pt;"><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> برای آنالیز آماری نتایج آزمایشات و ارزیابی آنها استفاده شد.</span></span></span></span><br>
<strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نتایج</span></span></strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> نتایج نشان داد که سامانه لیپوفکتامین 2000 به تنهایی هیچ سمیتی در هر دو رده سلولی </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Hum-63</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> و</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">SaOs-2</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> نداشت. سلول های سرطانی </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">SaOs-2</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> نسبت به سلول های </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Hum-63</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> حساسیت بیشتری در مقابل </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">miR-143</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> آزاد و</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Lipo/miR-143</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> از خود نشان دادند. بیشترین کاهش در سلول های </span></span></span> <span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">SaOs-2</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بعد از تیمار با </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Lipo<em>/</em>miR-143</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> مشاهده شد (</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p<0/05</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">). همچنین مطابق نتایج، ترانسفکشن سلولی </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">miR-143</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> توسط لیپوفکتامین 2000 در مقایسه با تیمار سلول ها با </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">miR-143</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> آزاد، باعث کاهش معنی داری در بیان ژن </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Bcl-2</span></span></span></span></em><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> شد (</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p<0/05</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">).</span></span></span><br>
<strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نتیجهگیری:</span></span></strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سامانه لیپیدی لیپوفکتامین 2000 می تواند در جذب سلولی </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">miR-143</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> موثر واقع شود.</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"> miR-143</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">از طریق هدف قرار دادن مسیرهای سلولی مختلفی از جمله میزان بیان ژن </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Bcl-2</span></span></span></span></em><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> می تواند در مسیر درمانی سرطان استخوان (استئوسارکوما) موثر واقع شود.</span></span></span></div>
<strong>Introdution:</strong> The present study was conducted to evaluate the effect of miR-143 on the expression of Bcl2 gene at the level of mRNA, cell cytotoxicity in the SaOs-2 cell line (Osteosarcoma) and Hum 63 (Human primary bone cells).<br>
<strong>Methods:</strong> In this experimental study, miR-143 was introduced into the SaOs-2 and Hum 63 cell by the lipofectam 2000 system. Then, the effects of miR-143 on cytotoxicity (72 hours) and expression of Bcl2 gene were measured by the MTT tests and Real Time Pcr protocols, respectively.<br>
<strong>Results: </strong>The results showed that the lipofectamine 2000 system had no toxicity in both Hum63 and SaOs2 cell lines by itself. SaOs2 cancer cells were more susceptible to miR-143 and Lipo / miR-143 compared to Hum63 cells. The highest reduction in SaOs2 cells was observed after the treatment with Lipo / miR-143 (p <0.05). Furthermore, according to the results, transfection of miR-143 by lipofectamine 2000 into the cell, compared to cell-treated alone by miR-143, significantly reduced Bcl2 expression (p <0.05).<br>
<strong>Conclusion:</strong> The lipoprotein 2000 system can be effective in absorbing miR-143 into the cells. By targeting various cellular pathways, including the expression level of the Bcl2 gene, miR-143 can be effective in the treatment of bone cancer (Osteosarcoma).
miR-143, لیپوفکتامین 2000, Bcl-2, استئوسارکوما
miR-143, Lipofectamin, Bcl-2, Osteosarcoma.
542
552
http://jssu.ssu.ac.ir/browse.php?a_code=A-10-303-5&slc_lang=fa&sid=1
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