The Journal of Shahid Sadoughi University of Medical Sciences
مجله علمي پژوهشي دانشگاه علوم پزشكي شهید صدوقی يزد
JSSU
Medical Sciences
http://jssu.ssu.ac.ir
20
journal20
2228-5741
2228-5733
fa
jalali
1397
5
1
gregorian
2018
8
1
26
5
online
1
fulltext
fa
مطالعه آزمایشگاهی اثر تیمار همزمان بیسفنولA و ویتامینC بر توانایی زیستی و تمایز استئوژنیک سلولهای بنیادی مزانشیمی مغز استخوان رت بالغ
In vitro study on the effect of Bisphenol A and vitamin C co-treatment on the viability and osteogenic differentiation of adult rat bone marrow mesenchymal stem cells
زیست شناسی
Biology
پژوهشي
Original article
<strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مقدمه:</span></span></strong> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بیسفنول </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">A</span></span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">از طریق تولید رادیکال­های آزاد، </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مورفولوژی، </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">توانایی زیستی و </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">تمایز سلولهای بنیادی مزانشیم مغز استخوان رت </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">(rMSCs)</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> به استئوبلاست را مختل می</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">کند. ویتامین </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">C</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> یک آنتی اکسیدانت قوی است و از سلول</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ها در مقابل استرس اکسیداتیو محافظت میکند. هدف از این مطالعه بررسی اثر تیمار همزمان بیسفنول</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">A</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> و ویتامین</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">C</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> به</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">عنوان یک آنتیاکسیدانت بر توانایی زیستی و تمایز استئوژنیک </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">rMSCs</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> بود.</span></span></span><br>
<strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">روش بررسی:</span></span></strong> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در این مطالعه تجربی </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">rMSCs</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> به چهار گروه کنترل، بیسفنول</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">A</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> (200نانومولار)، بیسفنول</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">A</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> (200نانومولار)+ ویتامین</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">C</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> (300میکرومولار) و ویتامین</span></span></span> <span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">C</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">(300میکرومولار) تقسیم شد و برای مدت 21 روز، در محیط کشت استئوژنیک، تیمار شد. سپس توانایی زیستی، تمایز استئوژنیک، تغییرات مورفولوژیک و شکستگی </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">DNA</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> در سلولهای گروههای مختلف مورد ارزیابی قرار گرفت. داده­ها </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">با</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">روش آنالیز</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آماری</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">واریانس</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">یک</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">طرفه </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">و تست </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Tukey</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> تجزیه و تحلیل و تفاوت میانگینها در سطح </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">P</span></span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"><</span></span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">0.05</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> معنیدار در</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نظر گرفته شد.</span></span></span><br>
<strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نتایج:</span></span></strong> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">کاهش معنی</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">­</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">داری در توانایی زیستی سلولها، میزان معدنی شدن ماتریکس استخوانی، فعالیت آنزیم آلکالین</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">­</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">فسفاتاز و غلظت کلسیم داخل سلولی</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> (</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">P</span></span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">< 0/00</span></span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">1</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">) و هم</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">چنین افزایش قابل</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">­</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">توجهی در شکستگی </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">DNA</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، در سلولهای گروه بیسفنول</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">A</span></span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نسبت به گروه کنترل مشاهده شد. </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">پارامترهای فوق، در گروه بیسفنول</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">A</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> + </span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ویتامین</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">C</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> نسبت به گروه کنترل تفاوت معنی</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">داری نداشت </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">(p> 0/05)</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">.</span></span></span><br>
<strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نتیجهگیری:</span></span></strong> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نتایج حاصل از این پژوهش نشان داد که ویتامین </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">C</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> قادر است اثرات نامطلوب بیسفنول</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">A</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> را بر توانایی زیستی و تمایز استئوژنیک </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">r</span></span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">MSCs</span></span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">جبران کند.</span></span></span>
<span style="color:#000000;"><strong>Introdution:</strong> Bisphenol A (BPA) disturbs the morphology, viability and differentiation of rat bone marrow mesenchymal stem cells (rMSCs) to osteoblast via the production of free radicals. Vitamin C (vit-C) is a potent antioxidant and protects cells against oxidative stress. The aim of this study was to investigate the effect of co-treatment of BPA and vitamin C as an antioxidant on the viability and osteogenic differentiation of rMSCs.<br>
<strong>Methods:</strong> In this experimental study, rMSCs were divided into 4 groups; control, BPA (200nM), BPA (200nM) + vit-C (300µM) as well as vit- C (300µM) and treated for 21 days in the osteogenic media. Then, cell viability, osteogenic differentiation, morphological changes and <cite>DNA breakage in different groups of cells were evaluated. Data were </cite>analyzed using one-way ANOVA and Tukey‘s test and the means were considered significantly different at P<0.05.<br>
<strong>Results:</strong> A significant reduction in the cell viability, bone matrix mineralization, alkalin phosphatase activity and intracellular calcium concentration (P<0.001) as well as a considerable increase in <cite>DNA breakage </cite>was seen in the BPA group compared to the control. The above parameters were compensated in the BPA + Vit-C group to the control level.<br>
<strong>Conclusion:</strong> The results of this investigation showed that Vit-C can compensate the adverse effects of BPA on the viability and osteogenic differentiation of rMSCs.</span>
بیسفنولA, ویتامینC, سلولهای بنیادی مزانشیم مغز استخوان رت, تمایز استئوژنیک
Bisphenol A ,Vitamin C,Rat Bone Marrow,Mesenchymal Stem Cells,Osteogenic Differentiation.
450
462
http://jssu.ssu.ac.ir/browse.php?a_code=A-10-3197-2&slc_lang=fa&sid=1
Malek
Soleimani Mehranjani
ملک
سلیمانی مهرنجانی