The Journal of Shahid Sadoughi University of Medical Sciences
مجله علمي پژوهشي دانشگاه علوم پزشكي شهید صدوقی يزد
JSSU
Medical Sciences
http://jssu.ssu.ac.ir
20
journal20
2228-5741
2228-5733
fa
jalali
1397
12
1
gregorian
2019
3
1
26
11
online
1
fulltext
fa
اثر تزریق عصاره الکلی فرولا اسزوویتسیانا بر روی نقایص شناختی و پرواکسیداسیون القاء شده توسط اتیدیوم بروماید در مدل تجربی MS
The effect of ethanolic extract of Ferula szowitsiana on cognitive deficits and lipid peroxidation induced by ethidium bromide in experimental model of MS
فیزیولوژی
Physiology
پژوهشي
Original article
<div></div> <strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مقدمه:</span></span></strong> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">هدف از </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مطالعه</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">حاضر، اثر</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ری</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ز</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">تزریق کوتاه مدت عصار</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ه</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> فرولااسزوویتسی</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ا</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نا بر روی حافظه فضایی و پروکسیداسیون لیپیدی در هیپوک</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ا</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مپ مدلهای تجربی مالتیپلاسکلروزیس (</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">MS</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">) است.</span></span><br>
<strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">روش بررسی:</span></span></strong> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در این مطالعه تجربی تعداد 35 سر رت نر نژاد ویستار به طور تصادفی به پنج گروه هفتتایی شامل کنترل، شاهد، مدل </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">MS</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، و دو گروه تیمار عصاره تقسیم شدند. در</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">گروه مدل تجربی </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">MS</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، القای</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">MS</span></span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> با تزریق سم اتیدیومبروماید به هیپوکامپ انجام گرفت. یک هفته پس از القای </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">MS</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، دو گروه تیمار به مدت 3 روز عصاره فرولااسزوویتسیانا را با دوزهای </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">g/ratµ</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">5 و </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">g/ratµ</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">10</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">دریافت کردند. پس </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">از</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">اتمام</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">دوره</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">تیمار،</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">جهت سنجش حافظه فضایی</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، آزمون</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ماز</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آبی</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">موریس انجام</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">گرفت</span></span><span style="font-family:b nazanin;">.</span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> در</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">خاتمه،</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بعد از </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نمونهبرداری</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">از</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">هیپوکامپ هر دوطرف و سنجش مالوندیآلدئید (</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">MDA</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">)، </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">دادهها به</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">صورت</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">میانگین</span></span> <span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">± </span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">خطای</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">استاندارد</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">میانگین (</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Mean±S.E.M</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">) </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ارائه</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">گردید و اختلاف</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">معنیدار</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">توسط</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آنالیز</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">واریانس</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">یکطرفه (</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">ANOVA</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">) با آزمون تعقیبی </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Student-Newman-Keuls </span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> به وسیله نرمافزار </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Instat3</span></span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مورد سنجش قرار گرفت.</span></span><br>
<strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نتایج:</span></span></strong> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نتایج نشان داد که مسافت طی شده (29/53</span></span><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">±</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">44/1022) و زمان (29/3</span></span><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">±</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">30/41) رسیدن به سکوی پنهان در گروه مدلهای </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">MS</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> نسبت به مسافت طی شده (56/29</span></span><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">±</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">94/885) و زمان (65/0</span></span><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">±</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> 26/36) گروه کنترل سالم افزایش یافت </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">(001/0</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">P</span></span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"><</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">) و تیمار کوتاه مدت عصاره فرولااسزوویتسیانا، مسافت (16/24</span></span><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">±</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> 39/838) و زمان (24/1</span></span><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">±</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> 87/39) رسیدن به سکو را در این مدلها </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">کاهش داد (001/0</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">P</span></span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"><</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">). میزان </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">MDA</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> مدلهای تجربی </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">MS</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> (51/0</span></span><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">±</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> 8/3) نسبت به گروه کنترل (13/0</span></span><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">±</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">68/0) افزایش یافت (001/0 </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">P<</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">) و در مدلهای </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">MS</span></span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">تحت</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">تیمار</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">با</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">عصاره</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">فرولا (04/0</span></span><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">±</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> 34/0)کاهش </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">یافت</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">(001/0 </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">P<</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">).</span></span><br>
<strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نتیجهگیری:</span></span></strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> تیمار</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">عصاره فرولااسزوویتسیانا</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">با</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">کاهش</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">میزان</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">محصولات</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">پروکسیداسیون</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">لیپیدی</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">قادر</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">به</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ممانعت</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">از</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">کاهش</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">روند</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">یادگیری</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">و</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">حافظه</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مدلهای تجربی </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">MS</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> است.</span></span><br>
<div></div>
<strong>Introduction:</strong> The aim of this study was to investigate the effect of short-term microinjection of Ferula szowitsiana extract on the process of spatial memory and lipid peroxidation in the hippocampus in an experimental model of Multiple Sclerosis (MS).<br>
<strong>Methods:</strong> In this experimental study, 35 male Wistar rats were randomly divided into five groups. Each group has 7 rats. These groups were included control, sham, model of MS and MS groups with plant extract treatments. In the experimental model of MS groups, induction of MS was carried out by single injection of ethidium bromide (EB) into the hippocampus. One week after MS induction by EB (0.01 %), the MS groups were treated by Ferula szowitsiana extract (5 and 10 μg/rat) for 3 consecutive days. Finally following the treatment period, for measuring spatial memory, Morris Water Maze test was carried out and the hippocampus of both sides were dissected and used for measurement of Malondialdehyde (MDA).<br>
<strong>Results:</strong> The results showed that in the experimental model of MS group travelled distance (1022.44±53.29) and escape latency (41.30±3.29) increased compared to travelled distance (885.94±29.56) and escape latency (36.26±0.65) in the control group (p<0.001). Short-term treatment by Ferula szowitsiana extract in this models decreased the travelled distance (838.39±24.16) and escape latency (39.87±1.24) (P<0.001). MDA increased in the experimental model of MS group (3.8±0.51) compared to the control group (0.68±0.13) (p<0.001) and in the Ferula treated group (0.34±0.04) decreased compared to the MS animals (P<0.001).<br>
<strong>Conclusion:</strong> Treatment with Ferula szowitsiana extract is able to prevent memory and learning reduction, through inhibition of lipid peroxidation in an experimental model of MS.<br>
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هیپوکمپ, مالتیپل اسکلروزیس, مالوندیآلدئید, فرولااسزوویتسیانا, حافظه فضایی, رت
Hippocampus, Multiple sclerosis, Malondialdehyde, Ferula szowitsiana, Spatial memory, rat.
1008
1018
http://jssu.ssu.ac.ir/browse.php?a_code=A-10-3440-1&slc_lang=fa&sid=1
Somayeh
Abdi
سمیه
عبدی
somayehabdi.2013b@gmail.com