The Journal of Shahid Sadoughi University of Medical Sciences
مجله علمي پژوهشي دانشگاه علوم پزشكي شهید صدوقی يزد
JSSU
Medical Sciences
http://jssu.ssu.ac.ir
20
journal20
2228-5741
2228-5733
fa
jalali
1397
5
1
gregorian
2018
8
1
26
5
online
1
fulltext
fa
بررسی اثر متابولیت های تولیدی سویه بومی Pseudomonas sp UW4 بر بیان ژن p53 در رده سلولی SK-BR-3 سرطان پستان انسان
Survey the effect of produced metabolites by native Pseudomonas sp. UW4 on p53 gene expression in SK-BR-3 breast cancer cell line
زیست شناسی
Biology
پژوهشي
Original article
<strong><span style="font-family:b nazanin;">مقدمه:</span></strong> <span style="color:#222222;"><span style="font-family:b nazanin;">با توجه به مشکلات در حال توسعه </span></span><span style="color:#222222;"><span style="font-family:b nazanin;">مسمومیت سیستمیک و مقاومت دارویی در شیمی</span></span> <span style="color:#222222;"><span style="font-family:b nazanin;">درمانی، کشف</span></span><span style="font-family:b nazanin;"> <span style="color:#222222;">ترکیبات</span></span> <span style="color:#222222;">فعال زیستی</span> و ضد<span dir="LTR"><span style="font-size:10.0pt;">­</span></span><span style="font-family:b nazanin;">سرطانی <span style="color:#222222;">جدید</span></span> <span style="color:#222222;">بسیار ضروری است. بنابراین در این مطالعه به بررسی فعالیت ضد­سرطانی متابولیت تولیدی سویه بومی </span><em><span dir="LTR"><span style="color:#222222;"><span style="font-size:10.0pt;">Pseudomonas</span></span></span></em><span dir="LTR"><span style="color:#222222;"><span style="font-size:10.0pt;"> sp. UW4</span></span></span> <span style="color:#222222;"><span style="font-family:b nazanin;"> در جهت شناسایی ترکیبات جدید پرداخته شد.</span></span><br>
<strong><span style="font-family:b nazanin;">روش بررسی:</span></strong> <span style="font-family:b nazanin;">مطالعه حاضر به صورت تجربی از اردیبهشت 1394 تا شهریور 1394 در دانشگاه آزاد اسلامی شهرکرد انجام شد. <span style="color:#222222;">ابتدا</span></span> <span style="color:#222222;">سلول­های</span> <span style="color:#222222;">سرطان پستان انسان</span><span dir="LTR"><span style="font-size:10.0pt;">SK-BR-3 </span></span><span style="font-family:b nazanin;"> <span style="color:#222222;">با غلظت­های مختلف</span></span> <span style="color:#222222;">(</span>5، 10 <span style="color:#222222;">و 20 میلیگرم/میلیلیتر)</span> <span style="color:#222222;">به مدت 24</span> <span style="color:#222222;">و 48 ساعت</span> <span style="color:#222222;">با متابولیت</span> <span style="color:#222222;">تولید</span> <span style="color:#222222;"><span style="font-family:b nazanin;">شده توسط سویه بومی </span></span><em><span dir="LTR"><span style="color:#222222;"><span style="font-size:10.0pt;">Pseudomonas</span></span></span></em><span dir="LTR"><span style="color:#222222;"><span style="font-size:10.0pt;"> sp. UW4</span></span></span><span style="color:#222222;"><span style="font-family:b nazanin;"> تیمارشدند </span></span><span style="font-family:b nazanin;">و</span><span style="font-family:b nazanin;"> میزان بقاء سلولی با روش رنگ</span><span dir="LTR"><span style="font-size:10.0pt;">­</span></span><span style="font-family:b nazanin;">سنجی </span><span dir="LTR"><span style="font-size:10.0pt;">MTS</span></span><span style="font-family:b nazanin;"> مورد بررسی قرار گرفت.</span><span style="font-family:b nazanin;"> سپس <span style="color:#222222;">بیان ژن</span></span> <em><span dir="LTR"><span style="font-size:10.0pt;">p53</span></span></em><span style="color:#222222;"><span style="font-family:b nazanin;"> با استفاده از روش</span></span> <span dir="LTR"><span style="font-size:10.0pt;">RT-Real Time PCR</span></span><span style="font-family:b nazanin;"> بررسی شد. <span style="letter-spacing:-.2pt;">جهت آنالیز آماری، از نرم افزار 16</span></span><span dir="LTR" style="letter-spacing:-.2pt;"><span style="font-size:10.0pt;">SPSS</span></span><span style="letter-spacing:-.2pt;"><span style="font-family:b nazanin;"> و آزمون آماری </span></span><span dir="LTR" style="letter-spacing:-.2pt;"><span style="font-size:10.0pt;">ANOVA</span></span><span style="letter-spacing:-.2pt;"><span style="font-family:b nazanin;"> یک طرفه استفاده شد.</span></span><br>
<strong><span style="font-family:b nazanin;">نتایج:</span></strong> <span style="color:black;"><span style="font-family:b nazanin;">تیمار با متابولیت تولیدی </span></span><span style="font-family:b nazanin;">سویه بومی </span><em><span dir="LTR"><span style="color:#222222;"><span style="font-size:10.0pt;">Pseudomonas </span></span></span></em><span dir="LTR"><span style="color:#222222;"><span style="font-size:10.0pt;">sp. UW4</span></span></span> <span style="font-family:b nazanin;">، <span style="color:black;">نشان داد</span> با</span> <span style="font-family:b nazanin;">افزایش</span> <span style="font-family:b nazanin;">غلظت به</span> <span style="font-family:b nazanin;">صورت</span> <span style="font-family:b nazanin;">وابسته</span> <span style="font-family:b nazanin;">به</span> <span style="font-family:b nazanin;">دوز</span> <span style="font-family:b nazanin;">و</span> <span style="font-family:b nazanin;">زمان،</span> <span style="font-family:b nazanin;">توان</span> <span style="font-family:b nazanin;">زیستی</span> <span style="font-family:b nazanin;">سلول­ها</span> <span style="font-family:b nazanin;">کاهش</span> <span style="font-family:b nazanin;">پیدا کرد</span><span style="font-family:b nazanin;">ه است، </span><span style="font-family:b nazanin;">به</span> <span style="font-family:b nazanin;">طوری</span> <span style="font-family:b nazanin;">که بیشترین</span> <span style="font-family:b nazanin;">تأثیر</span> <span style="font-family:b nazanin;">مربوط</span> <span style="font-family:b nazanin;">به</span> <span style="font-family:b nazanin;">غلظت</span> <span style="font-family:b nazanin;">۲۰ میلی­گرم/میلی­لیتر</span> <span style="font-family:b nazanin;">و</span> <span style="font-family:b nazanin;">در</span> <span style="font-family:b nazanin;">زمان</span> <span style="font-family:b nazanin;">48 ساعت</span> <span style="font-family:b nazanin;">پس</span> <span style="font-family:b nazanin;">از</span> <span style="font-family:b nazanin;">تیمار</span> <span style="font-family:b nazanin;">سلول­ها</span> <span style="font-family:b nazanin;">بود. <span style="color:black;">هم</span></span> <span style="color:black;"><span style="font-family:b nazanin;">چنین افزایش بیان ژن </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">p53</span></span></em><span style="font-family:b nazanin;"> <span style="color:black;">به ویژه در غلظت­های 10 و 20</span></span> <span style="color:#222222;"><span style="font-family:b nazanin;">میلیگرم/میلیلیتر</span></span><span style="color:black;"><span style="font-family:b nazanin;"> بعد از 24 ساعت و در </span></span><span style="color:black;"><span style="font-family:b nazanin;">غلظت­های 5، 10 و 20</span></span><span style="color:#222222;"><span style="font-family:b nazanin;">میلیگرم/میلیلیتر</span></span><span style="color:black;"><span style="font-family:b nazanin;"> بعد از 48 ساعت مشاهده شد </span></span><span dir="LTR"><span style="font-size:10.0pt;">(P<0/05)</span></span><span style="font-family:b nazanin;">. </span><br>
<strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نتیجهگیری:</span></span></strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> می­توان از <span style="color:#222222;">متابولیت</span></span> <span style="color:#222222;">تولید شده توسط سویه بومی </span></span><em><span dir="LTR"><span style="color:#222222;">Pseudomonas</span></span></em><span dir="LTR"><span style="color:#222222;"> sp. UW4</span></span><span style="color:#222222;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> در درمان سرطان پستان </span></span></span><span dir="LTR">SK-BR-3</span><span style="color:#222222;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> استفاده کرد</span></span></span><span style="font-family:b koodak;"><span style="font-size:9.0pt;">.</span></span>
<strong>Introdution:</strong> Regarding to the development the problems of systemic toxicity and drug resistance in cancer chemotherapy, the continuing discovery of new bioactive compounds and anticancer agents is very necessary. Therefore, in this study, we checked the anticancer activity of native <em>Pseudomonas</em> sp. UW4 metabolite to find new compound.<br>
<strong>Methods:</strong> This experimental study was performed in Shahrekord Islamic Azad University from April 2015 to August 2015<span dir="RTL">.</span> First SK-BR-3 human breast cancer cells with different concentrations (5, 10 and 20 mg/ml) were treated with produced metabolite by native <em>Pseudomonas</em> sp. UW4 for 24 and 48 hours. Cell viability was assessed by MTS assay. Then, <em>p53 </em>expression was detected by RT-Real Time PCR. For statistical analysis, SPSS16 software and one way ANOVA test were used.<br>
<strong>Results:</strong> Treatment with produced metabolite by the native <em>Pseudomonas </em>sp. UW4 showed the decreases the viability of cells in a time and dose dependent manner, the most effective concentration of this substance was 20 mg/ml and 48 h after treatment. Also, an increase in <em>p53 </em>gene expression, significantly in 10 and 20 mg/ml after 24 h and 5, 10 and 20 mg/ml after 48h was observed (P<0.05).<br>
<strong>Conclusion:</strong> Produced metabolite by native <em>Pseudomonas</em> sp. UW4 could be used for treatment of SK-BR-3 breast cancer.
Pseudomonas sp.UW4, متابولیت, سرطان پستان SK-BR-3, توان زیستی, ژن p53,Real Time PCR RT-
Pseudomonas sp.UW4, Metabolite, Breast cancer SK-BR-3, Cell viability, p53 gene, RT-Real Time PCR
399
409
http://jssu.ssu.ac.ir/browse.php?a_code=A-10-2961-2&slc_lang=fa&sid=1
Setareh
Pourhavashemi
ستاره
پورهواشمی
starhavashemi@yahoo.com