The Journal of Shahid Sadoughi University of Medical Sciences
مجله علمي پژوهشي دانشگاه علوم پزشكي شهید صدوقی يزد
JSSU
Medical Sciences
http://jssu.ssu.ac.ir
20
journal20
2228-5741
2228-5733
fa
jalali
1395
11
1
gregorian
2017
2
1
24
11
online
1
fulltext
fa
نقش پلیمورفیسمrs7895833 SIRT-1 در استعداد ابتلا به سندروم تخمدان پلیکیستیک
Role of SIRT-1 rs7895833 polymorphism in susceptibility to polycystic ovary syndrome
ژنتیک
Genetics
پژوهشي
Original article
<p dir="RTL" style="margin-bottom:0cm;margin-bottom:.0001pt;text-align:justify;text-indent:11.35pt;line-height:20.0pt;direction:rtl;unicode-bidi:embed;"><strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مقدمه</span></span></strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">: سیرتوئین-1 (</span></span><span dir="LTR"><span style="font-family:times new roman,serif;">SIRT-1</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">) یک پروتئین داستیلاز است که نقش مهمی در بسیاری از فرایندهای سلولی دارد. در زنان مبتلا به سندروم تخمدان پلیکیستیک (</span></span><span dir="LTR"><span style="font-family:times new roman,serif;">PCOS</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">) سطح سرمی</span></span> <span dir="LTR"><span style="font-family:times new roman,serif;">SIRT-1</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> نسبت به زنان سالم بالاتر است. هدف این مطالعه، بررسی نقش پلیمورفیسم </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">SIRT-1 rs7895833</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> در استعداد ابتلا به </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">PCOS</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> است.</span></span></p>
<p dir="RTL" style="margin-bottom:0cm;margin-bottom:.0001pt;text-align:justify;text-indent:11.35pt;line-height:20.0pt;direction:rtl;unicode-bidi:embed;"><strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">روش بررسی:</span></span></strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> این مطالعه مورد-شاهدی بر روی 456 زن ایرانی در فاصله زمانی فروردین تا اسفند 1394 صورت گرفت. نمونه خون از 274 زن مبتلا به </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">PCOS</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> و 182 زن سالم در بیمارستان مادر و کودک غدیر شیراز که از لحاظ سن (5</span></span><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">±</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">) همسانسازی شدند، جمعآوری شد. پس از استخراج </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">DNA</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، تعیین ژنوتیپ </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">SIRT-1</span></span> <span dir="LTR"><span style="font-family:times new roman,serif;">rs7895833</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> با روش </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">Tetra</span></span><span dir="LTR"><span style="font-size:12.0pt;">-</span></span><span dir="LTR"><span style="font-family:times new roman,serif;">ARMS</span></span> <span dir="LTR"><span style="font-family:times new roman,serif;">PCR</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> انجام گرفت. جهت تجزیه و تحلیل اطلاعات از نرمافزار </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">SPSS</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> ویرایش 18 استفاده شد.</span></span></p>
<p dir="RTL" style="margin-bottom:0cm;margin-bottom:.0001pt;text-align:justify;text-indent:11.35pt;line-height:20.0pt;direction:rtl;unicode-bidi:embed;"><strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نتایج</span></span></strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">: در مدل غالب برای آلل </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">G</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> (مقایسه بین </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">AG</span></span><span dir="LTR"><span style="font-size:12.0pt;">+</span></span><span dir="LTR"><span style="font-family:times new roman,serif;">GG</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> در مقابل </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">AA</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">)، ژنوتیپ­های </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">AG</span></span><span dir="LTR"><span style="font-size:12.0pt;">+</span></span><span dir="LTR"><span style="font-family:times new roman,serif;">GG</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> پلیمورفیسم </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">rs7895833</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> در ژن </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">SIRT-1</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> با افزایش خطر ابتلا به </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">PCOS</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> همراه بودند (0/002</span></span><span style="font-family:times new roman,serif;">=</span><span dir="LTR"><span style="font-family:times new roman,serif;">p</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، 3/24-1/23</span></span><span style="font-family:times new roman,serif;">:</span><span dir="LTR"><span style="font-family:times new roman,serif;">CI</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> 95%، 2/01:</span></span><span dir="LTR"><span style="font-family:times new roman,serif;">OR</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">). بعلاوه، ارتباط معنی</span></span><span style="font-family:arial,sans-serif;"><span style="font-size:12.0pt;"></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">داری بین آلل</span></span> <span dir="LTR"><span style="font-family:times new roman,serif;">G</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> و ابتلا به </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">PCOS</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> وجود داشت (0/008</span></span><span style="font-family:times new roman,serif;">=</span><span dir="LTR"><span style="font-family:times new roman,serif;">p</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، 1/19-1/101:</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">CI</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> 95%، 1/45:</span></span><span dir="LTR"><span style="font-family:times new roman,serif;">OR</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">).</span></span></p>
<p dir="RTL" style="margin-bottom:0cm;margin-bottom:.0001pt;text-align:justify;text-indent:11.35pt;line-height:20.0pt;direction:rtl;unicode-bidi:embed;"><strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نتیجهگیری</span></span></strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">: نتایج مطالعه ارتباط احتمالی پلیمورفیسم </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">rs7895833</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> ژن</span></span> <span dir="LTR"><span style="font-family:times new roman,serif;">SIRT-1</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">و استعداد ابتلا به </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">PCOS</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> را نشان دادند.</span></span></p>
<p dir="RTL" style="margin-bottom:0cm;margin-bottom:.0001pt;text-align:justify;text-indent:11.35pt;line-height:20.0pt;direction:rtl;unicode-bidi:embed;"></p>
<p style="text-align: justify;"><strong><em>Introduction</em></strong>: Sirtuin 1 is a protein deacetylase that plays an important role in many cellular processes. The serum sirtuin 1 levels are higher in the polycystic ovary syndrome (PCOS) patients than the healthy subjects. The aim of this study was to assess the role of SIRT-1 rs7895833 polymorphism in the susceptibility of PCOS.</p>
<p style="text-align: justify;"><strong><em>Methods</em></strong><strong>:</strong> This case-control study was done on 456 Iranian women from March to February 2015. Blood samples were collected from 274 women with PCOS and 182 age matched (±5) healthy women at Shiraz Ghadir Mother and Child Hospital. After DNA extraction, SIRT1 rs7895833 genotype determination was done by Tetra-ARMS PCR. Data analysis was performed by SPSS version 18.</p>
<p style="text-align: justify;"><strong><em>Results</em></strong>: In the dominant model for G allele (AG+GG vs. AA), AG+GG genotypes in SIRT1 rs7895833 gene polymorphism was associated with the increased risk of PCOS (OR: 2.01, %95CI:1.32-3.24, P=0.002). Moreover, there was a significant association between the G allele and PCOS (OR: 1.45, %95CI:1.10-1.91, P=0.008).</p>
<p style="text-align: justify;"><strong><em>Conclusion:</em></strong> The results showed a possible association between SIRT1 rs7895833 gene polymorphism and susceptibility to PCOS.</p>
سندروم تخمدان پلیکیستیک, SIRT-1, چاقی, پلیمورفیسم
Polycystic Ovary Syndrome, SIRT1, Obesity, Polymorphism
861
867
http://jssu.ssu.ac.ir/browse.php?a_code=A-10-2936-1&slc_lang=fa&sid=1
Zahra
Rezaei
زهرا
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