Volume 13, Issue 4 (Atumn 2005)
JSSU 2005, 13(4): 76-81 |
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Dehghani - Ashkezari M, Kalantar M, Parivar K, Aflatoonian A. Predictability Power of Acrosome Reaction Test in Ivf Treatment Cycles. JSSU 2005; 13 (4) :76-81
URL: http://jssu.ssu.ac.ir/article-1-707-en.html
URL: http://jssu.ssu.ac.ir/article-1-707-en.html
Abstract: (8467 Views)
Introduction: It is well known that one of the powerful sperm function tests is the acrosome reaction (AR),
which is a prerequisite in the fertilization process. The predictability of sperm fertilizing ability using AR has
been suggested for IVF treatment cycles. The aim of study was to assess the power of AR using human
follicular fluid (hFF) to predict the fertilization rate (FR) in IVF cycles.
Materials &Methods: This investigation was experimental. During 9 month, 54 different semen samples
were collected from infertile men exactly before insemination of retrieved oocytes. Each sample was divided
into 4 aliquots and semen analysis (SA) was done on the first aliquot. For Acrosomal reaction, the sperm
samples were washed with Ham'sf10 culture media and after 2 hours in 37C incubator, the samples were
divided into 3 tubes. The first tube was control, DMSO 1mg/ml was added to the second tube and follicular
fluid was added to the third one. The acrosome was stained by double staining method and acrosomal status
was examined.
Results: The data analysis showed that there are no significant relationships between fertilization, sperm
count, fast moving sperms, slow moving sperms, overall sperm motility and morphology. The results also
showed that the mean of acrosome reactions in groups with rate 50 and > 50% were significant (p<0.05). In
addition, using ROC analysis, with cut-off value of 45% for fertilization, a cut-off value of 10.5% was
achieved.
Conclusion: In order to have a more accurate selection of the method of fertilization, predict the success rate
of IVF and prevent possible complications, it is advisable to use acrosome reaction test
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