دوره 13، شماره 4 - ( پاییز 1384 )
جلد 13 شماره 4 صفحات 75-71 |
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Dehghani F, Dezfulian A, Panjehshahin M, Zahiri S. Estimation of Glomerular Volume in Rats with Glycerol-Induced Acute Renal Failure. JSSU 2005; 13 (4) :71-75
URL: http://jssu.ssu.ac.ir/article-1-699-fa.html
URL: http://jssu.ssu.ac.ir/article-1-699-fa.html
دهقانی فرزانه، دزفولیان عبدالرحمن، پنجه شاهین محمد رضا، ظهیری شهلا. تخمین معدل حجمی گلومرول ها در نارسایی حاد کلیه القا شده توسط گلیسرول در موش صحرایی نر. مجله علمي پژوهشي دانشگاه علوم پزشكي شهید صدوقی يزد. 1384; 13 (4) :71-75
چکیده: (7285 مشاهده)
Introduction: The use of stereological methods in evaluation of volume changes in vital organs such as
kidney is very important. In this study, the volume of glomeruli in glycerol-induced acute renal failure in rats
was determined.
Methods: 48 Sprague-Dawley male rats were divided into 2 groups (1control, 1experiment). Experimental
group was injected 10ml/kg of 50% glycerol intramuscularly, while the same volume of saline was injected
in the control group. After 48h, animals were dissected under deep anesthesia, and right kidneys were fixed
by vascular perfusion. Microscopic slides were stained with H-E. Glomerular volumes were calculated using
projecting microscope, point counting and cavalieri principal.
Results: The results indicated that renal failure induced-glycerol didn’t affect the glomerular volume
Conclusion: It seems that estimation of glomerular volume can not be a useful variable in identification of
acute renal failure induced glycerol, but further investigations are needed to clarify the issue
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