Volume 26, Issue 5 (Agu 2018)
JSSU 2018, 26(5): 385-392 |
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Abstract: (3315 Views)
Introduction: In the recent decades, the prevalence of Klebsiella pneumoniae beta-lactamase producing strains has a significant role in nosocomial infections. The aim of this study was to determine the prevalence of different types of CTX-M enzymes among clinical isolates of K. pneumoniae.
Methods: In this descriptive cross-sectional study, during six months, K. pneumoniae strains were isolated from laboratory ward of Babol educational hospitals and referred to microbiology laboratory of Babol University of Sciences for final confirmation. Thereafter, antibiogram pattern analysis and the frequency of CTX-M1 and CTX-M15 genes were evaluated. Finally, the results were analyzed by SPSS version 24 software.
Results: During six months of this study in 2017, 65 K. pneumoniae and also other bacterial strains such as Escherichia coli, Pseudomonas aeruginosa, and Acinetobacter baumannii were collected from laboratory ward of Shahid Beheshti and Ayatollah Rouhani Hospitals. By disc diffusion method, the highest resistance belonged to Amoxicillin (91%), Cefoxitin (45%), Piperacillin (45%) and the lowest belonged to Amikacin (24%) and Imipenem (0%). On the other hand, the percentage of CTX-M1 and CTX-M15 genes were also found to be 62% and 69%, respectively.
Conclusion: Regarding to the high prevalence of strains containing broad-spectrum beta-lactamase enzymes and due to the antibiotic resistance among K. pneumoniae strains isolated from clinical specimens and also by considering this issue that the current study was the first research for evaluating the frequency of these genes in Babol educational hospitals therefore, it is necessary to adopt appropriate drug regimens to reduce antibiotic resistances
Methods: In this descriptive cross-sectional study, during six months, K. pneumoniae strains were isolated from laboratory ward of Babol educational hospitals and referred to microbiology laboratory of Babol University of Sciences for final confirmation. Thereafter, antibiogram pattern analysis and the frequency of CTX-M1 and CTX-M15 genes were evaluated. Finally, the results were analyzed by SPSS version 24 software.
Results: During six months of this study in 2017, 65 K. pneumoniae and also other bacterial strains such as Escherichia coli, Pseudomonas aeruginosa, and Acinetobacter baumannii were collected from laboratory ward of Shahid Beheshti and Ayatollah Rouhani Hospitals. By disc diffusion method, the highest resistance belonged to Amoxicillin (91%), Cefoxitin (45%), Piperacillin (45%) and the lowest belonged to Amikacin (24%) and Imipenem (0%). On the other hand, the percentage of CTX-M1 and CTX-M15 genes were also found to be 62% and 69%, respectively.
Conclusion: Regarding to the high prevalence of strains containing broad-spectrum beta-lactamase enzymes and due to the antibiotic resistance among K. pneumoniae strains isolated from clinical specimens and also by considering this issue that the current study was the first research for evaluating the frequency of these genes in Babol educational hospitals therefore, it is necessary to adopt appropriate drug regimens to reduce antibiotic resistances
Type of Study: Original article |
Subject:
Microbiology
Received: 2017/12/25 | Accepted: 2018/01/20 | Published: 2018/10/22
Received: 2017/12/25 | Accepted: 2018/01/20 | Published: 2018/10/22
References
1. Forbes BA, Sahm DF, Weissfeld AS. Bailey & Scott’s Diagnostic Microbiology. UK: Mosby; 2007.
2. Podschun R, Pietsch S, Höller C, Ullmann U. Incidence of Klebsiella species in surface waters and their expression of virulence factors. Appl Environment Microbio 2001; 67: 3325-27.
3. Calfee DP. Recent advances in the understanding and management of Klebsiella pneumoniae. F1000Res 2017; 27: 1760.
4. Zamani A, Yousefi Mashouf R, Ebrahimzadeh Namvar AM, Alikhani MY. Detection of magA Gene in Klebsiella spp. Isolated from Clinical Samples Detection of magA. Iran J Basic Med Sci 2013; 16: 173-6.
5. Hendrik TC, Voor In 't Holt AF, Vos MC. Clinical and Molecular Epidemiology of Extended-Spectrum Beta-Lactamase-Producing Klebsiella spp.: A Systematic Review and Meta-Analyses. PLoS One 2015; 10(10): e0140754.
6. Ku YH, Chuang YC, Chen CC, Lee MF, Yang YC, Tang HJ, et al. Klebsiella pneumoniae Isolates from Meningitis: Epidemiology, Virulence and Antibiotic Resistance. Scientific Reports 2017; 7: 6634.
7. Veras DL, Lopes AC, da Silva GV, Gonçalves GG, de Freitas CF, de Lima FC, et al. Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes bla SHV, bla TEM, bla CTX-M, or bla KPC When Subject to β-Lactam Antibiotics. Scientific World J 2015; 2015: 572128.
8. Bonnet R. Growing group of extended-spectrum β-lactamases: the CTX-M enzymes. Antimicrob Agents Chemother 2004; 48(1): 1-14.
9. Tzouvelekis LS, Tzelepi E, Tassios PT, Lagakis NJ. CTXM–type β-lactamases: an emerging group of extended-spectrum enzymes. Inter J Antimicrobial Agents 2000; 14(2): 137-42.
10. Cantón R, Coque M. The CTX-M β-lactamase pandemic. Current Opinion in Microbiology. Curr Opin Microbiol2006; 9: 466-75.
11. Calbo E, Freixas N, Xercavins M, Riera M, Nicolás C, Monistrol O, et al. Foodborne nosocomial outbreak of SHV1 and CTX-M-15-producing Klebsiella pneumoniae: epidemiology and control. Clin Infect Dis 2011; 52(6): 743-9.
12. Peerayeh SN, Rostami E, Siadat SD, Derakhshan S. High rate of aminoglycoside resistance in CTX-M-15 producing Klebsiella pneumoniae isolates in Tehran, Iran. Lab Med 2014; 45(3): 231-7.
13. Flores C CPA, Romão CM, Bianco K, Chaia de Miranda C, Breves A, Paula S, Souza A, et al. Detection of antimicrobial resistance genes in betalactamase- and carbapenemase-producing Klebsiella pneumoniae by patient surveillance cultures at an intensive care unit in Rio de Janeiro, Brazil. Brazilian J Pathology Laboratory Med 2016; 5: 284-92.
14. Conceição T, Brízio A, Duarte A, Lito LM, Cristino JM, Salgado MJ. First description of CTX-M-15-producing Klebsiella pneumoniae in Portugal. Antimicrobial Agents and Chemotherapy. 2005; 49(1): 477-78.
15. Soge OO, Queenan AM, Ojo KK, Adeniyi BA, Roberts MC. CTX-M-15 extended-spectrum (beta)-lactamase from Nigerian Klebsiella pneumoniae. J Antimicrobial Chemotherapy 2006; 57(1): 24-30.
16. Melegh S, Schneider G, Horváth M, Jakab F, Emődy L, Tigyi Z. Identification and characterization of CTX-M-15 producing Klebsiella pneumoniae clone ST101 in a Hungarian university teaching hospital. Acta Microbiologica Et Immunologica Hungarica 2015; 62(3): 233-45.
17. Amiri A, Firoozeh F, Moniri R, Zibaei M. Prevalence of CTX-M-Type and PER Extended-Spectrum β-Lactamases among Klebsiella spp. Isolated From Clinical Specimens in the Teaching Hospital of Kashan, Iran. Iran Red Crescent Med J 2016; 18(3): e22260.
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