Volume 25, Issue 7 (oct 2017)
JSSU 2017, 25(7): 547-555 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Karimi M N, Abbasalipourkabir R, Arab Sadeghabadi Z, Ziamajidi N. The level of gene expression of Bax and Bcl-2 and the activity of caspase 3 in the liver tissues of normal, type 1 and type 2 diabetic rats before and after treatment with aqueous extract of garlic
. JSSU 2017; 25 (7) :547-555
URL: http://jssu.ssu.ac.ir/article-1-4197-en.html
URL: http://jssu.ssu.ac.ir/article-1-4197-en.html
Abstract: (6360 Views)
Introduction: One of the complications of diabetes mellitus is induction of apoptosis in liver tissues. Activation of caspase 3 caused apoptosis in the cells. Garlic is a most common plant in diet that its effect on apoptosis was not determined. In this study the effects of this extract on Bax and Bcl-2 gene expression and the activity of caspase 3, were determined in type 1 and 2 of diabetes in rats.
Methods: Wistar rats divided into six groups included normal control rats, diabetes type 1 (D1), diabetes type 1 treated with garlic extract (D1G), diabetes type 2 (D2), diabetes type 2 treated with garlic extract (D2G) and garlic control (G). At the end of treatment time, liver tissues were removed quickly and stored
in -70 °C. For evaluation of gene expression, RNA extraction, cDNA synthesis and Real time PCR were done. The activity of caspase 3 was determined with flourometric method.
Results: The level of gene expression of Bax and Bcl-2, did not change significantly in different groups. The activity of caspase 3 in D1 rats, increased significantly compared to the control rats(p=0.003). The activity of this enzyme was high in D1G compared to control rats yet (p=0.021). In D2, D2G and G groups, caspase activity was not changed in comparison with the control rats
Conclusion: Diabetes mellitus type1 caused apoptosis in rat livers, but garlic extract cannot ameliorate it.
Methods: Wistar rats divided into six groups included normal control rats, diabetes type 1 (D1), diabetes type 1 treated with garlic extract (D1G), diabetes type 2 (D2), diabetes type 2 treated with garlic extract (D2G) and garlic control (G). At the end of treatment time, liver tissues were removed quickly and stored
in -70 °C. For evaluation of gene expression, RNA extraction, cDNA synthesis and Real time PCR were done. The activity of caspase 3 was determined with flourometric method.
Results: The level of gene expression of Bax and Bcl-2, did not change significantly in different groups. The activity of caspase 3 in D1 rats, increased significantly compared to the control rats(p=0.003). The activity of this enzyme was high in D1G compared to control rats yet (p=0.021). In D2, D2G and G groups, caspase activity was not changed in comparison with the control rats
Conclusion: Diabetes mellitus type1 caused apoptosis in rat livers, but garlic extract cannot ameliorate it.
Type of Study: Original article |
Subject:
Biochemistry
Received: 2017/05/11 | Accepted: 2017/06/3 | Published: 2017/11/14
Received: 2017/05/11 | Accepted: 2017/06/3 | Published: 2017/11/14
References
1. Ghamarian A, Abdollahi M, Su X, Amiri A, Ahadi A, Nowrouzi A. Effect of chicory seed extract on glucose tolerance test (GTT) and metabolic profile in early and late stage diabetic rats. Daru 2012; 20(1): 56. [persion]
2. Tripathi V, Verma J. Different models used to induce diabetes: A Comprehensive Review. Int J Pharm Pharm Sci 2014; 6(6): 29-32.
3. Robertson RP. Chronic oxidative stress as a central mechanism for glucose toxicity in pancreatic islet beta cells in diabetes. J Biol Chem 2004; 279(41): 42351-4.
4. Schattenberg JM, Schuchmann M. Diabetes and apoptosis: liver . Apoptosis 2009; 14(12): 1459-71.
5. Bayir H, Kagan VE. Bench-to-bedside review:Mitochondrial injury, oxidative stress and apoptosis– there is nothing more practical than a good theory.Crit Care 2008; 12(1): 206.
6. He B, Lu N, Zhou Z. Cellular and nuclear degradation during apoptosis. Curr Opin Cell Biol 2009; 21(6): 900-12.
7. Ide N, Lau BH. Garlic compounds minimize intracellular oxidative stress and inhibit nuclear factor-κB activation. The J nutrition 2001; 131(3): 1020S-6S.
8. Masjedi F, Gol A, Dabiri S. Preventive effect of garlic (Allium sativum L.) on serum biochemical factors and histopathology of pancreas and liver in streptozotocin-induced diabetic rats. Iran J Pharm Res. 2013; 12(3): 325-38.
9. El-Demerdash F, Yousef M, El-Naga NA. Biochemical study on the hypoglycemic effects of onion and garlic in alloxan-induced diabetic rats. Food Chem Toxicol 2005; 43(1): 57-63.
10. Brownlee M. Biochemistry and molecular cell biology of diabetic complications. Nature 2001; 414(6865): 813-20.
11. Robertson RP. Chronic oxidative stress as a central mechanism for glucose toxicity in pancreatic islet beta cells in diabetes. J Biol Chem 2004; 279(41): 42351-4.
12. Frances DE, Ronco MT, Monti JA, Ingaramo PI, Pisani GB, Parody JP, et al. Hyperglycemia induces apoptosis in rat liver through the increase of hydroxyl radical: new insights into the insulin effect. J Endocrinol 2010; 205(2): 187-200.
13. Reed JC. Bcl-2 and the regulation of programmed cell death. J Cell Biol 1994; 124(1-2): 1-6.
14. Chang C-C, Chang C-Y, HuangJ-P, Hung L-M. Effect of resveratrol on oxidative and inflammatory stress in liver and spleen of streptozotocin-induced type 1 diabetic rats. Chin J Physiol 2012; 55(3): 192-201.
15. Ingaramo PI, Ronco MT, Francés DE, Monti JA, Pisani GB, Ceballos MP, et al. Tumor necrosis factor alpha pathways develops liver apoptosis in type 1 diabetes mellitus. Mol Immunol 2011; 48(12-13): 1397-407.
16. Dias AS, Porawski M, Alonso M, Marroni N, Collado PS, González-Gallego J. Quercetin decreases oxidative stress, NF-κB activation, and iNOS overexpression in liver of streptozotocin-induced diabetic rats. The J nutrition 2005; 135(10): 2299-304.
17. Nasiri A, Ziamajidi N, Abbasalipourkabir R, Goodarzi MT, Saidijam M, Behrouj H, et al. Beneficial Effect of Aqueous Garlic Extract on Inflammation and Oxidative Stress Status in the Kidneys of Type 1 Diabetic Rats. Indian J Clin Biochem 2017; 32(3): 329-336.
18. Ziamajidi N, Nasiri A, Abbasalipourkabir R, Sadeghi Moheb S. Effects of garlic extract on TNF-α expression and oxidative stress status in the kidneys of rats with STZ+ nicotinamide-induced diabetes. Pharm Biol 2017; 55(1): 526-31.
19. Kapoor R, Kakkar p. Naringenin accords hepatoprotection from streptozotocin induced diabetes in vivo by modulating mitochondrial dysfunction and apoptotic signaling cascade. Toxicol Rep 2014; 1: 569-581.
20. Alabdan MA. Silimarin ameliorates liver damage by modulating oxidative stress and apoptosis in liver of STZ-induced diabetic rats. Int J Adv Res 2016; 4(3): 1935-1942.
21. Giribabu N, Karim K, Kilari EK, Kassim NM, Salleh N. Anti-Inflammatory, anti-apoptotic and pro-proliferative effects of Vitis Vinifera seed ethanolic extract in the liver of streptozotocin-nicotinamide-induced diabetes in male rats. Can J Diabetes 2017. Article in press.
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
