Volume 26, Issue 8 (Nov 2019)                   JSSU 2019, 26(8): 662-670 | Back to browse issues page

XML Persian Abstract Print

Abstract:   (1796 Views)
Introdution: The aim of this study was to investigate the effect of vitamin D supplementation on Klotho levels of the brain tissue.
Methods: In this laboratory experimental study, 25 female Lewis rats, after 2 weeks adaptation to lab environmental, were randomly divided into 5 groups: 1-control; 2- control+ EAE; 3-sham; 4vitamin D; 5-vitaminD + EAE.
Results: The data were analyzed  using one way ANOVA, and to examine the weight changes Mann-Whitney test was used to the research groups. In order to induce EAE, 0.4 ml of solution, a combination of guinea Hindi pig spinal cord and adjuvant ratio was injected to each animal. Klotho levels were measured by ELISA.
Finding: There was significantly different in Klotho levels of brain tissue of control+ EAE compared to control group (P=0/001). There was significant difference in Klotho levels of the brain tissue in EAE +vitamin D group compared to control group (P=0/001). In addition, the weight of vitamin D+EAE group was more than control+EAE group (P=0/006).
Conclusion: Pre-treatment with vitamin D  supplementation increases the Klotho levels of brain tissue and may have protective role against EAE.
Full-Text [PDF 972 kb]   (570 Downloads)    
Type of Study: Original article | Subject: Neurology
Received: 2017/04/10 | Accepted: 2018/06/23 | Published: 2018/12/25

2. 1- Dendrou CA, Fugger L, Friese MA. Immunopathology of multiple sclerosis. Nature Reviews Immunology 2015; 15: 545-58.
3. 2- Jin JJ, Ko IG, Kim SE, Shin MS, Kim SH, Jee YS. Swimming exercise ameliorates multiple sclerosis-induced impairment of short-term memory by suppressing apoptosis in the hippocampus of rats. J Exer Rehabil 2014; 10(2): 69-74.
4. 3- Steinman L. Multiple sclerosis: a coordinated immunological attack against myelin in the central nervous system. Cell 1996; 85(3): 299-302.
5. 4- Tasset I, Agüera E, Sánchez-López F, Feijóo M, Giraldo AI, Cruz AH, et al. Peripheral oxidative stress in relapsing–remitting multiple sclerosis. Clin Biochem 2012; 45(6): 440-4.
6. 5- Aleagha MSE, Siroos B, Ahmadi M, Balood M, Palangi A, Haghighi AN, et al. Decreased concentration of Klotho in the cerebrospinal fluid of patients with relapsing–remitting multiple sclerosis. J Neuroimmunology 2015; 281: 5-8.
7. 6- Zeldich E, Chen C-D, Colvin TA, Bove-Fenderson EA, Liang J, Zhou TBT, et al. The neuroprotective effect of Klotho is mediated via regulation of members of the redox system. J Biological Chem 2014; 289(35): 24700-15.
8. 7- Teocchi MA, Ferreira AÉD, de Oliveira EPdL, Tedeschi H, D’Souza-Li L. Hippocampal gene expression dysregulation of Klotho, nuclear factor kappa B and tumor necrosis factor in temporal lobe epilepsy patients. J Neuroinflammation 2013; 1;(10):53.
9. 8- Gilgun-Sherki Y, Melamed E, Offen D. The role of oxidative stress in the pathogenesis of multiple sclerosis: the need for effective antioxidant therapy. J Neurol 2004; 251(3): 261-8.
10. 9- Schwarz S, Leweling H. Multiple sclerosis and nutrition. Multiple Scler 2005; 11(1): 24-32.
11. 10- Van Etten E, Branisteanu D, Overbergh L, Bouillon R, Verstuyf A, Mathieu C. Combination of a 1, 25-dihydroxyvitamin D 3 analog and a bisphosphonate prevents experimental autoimmune encephalomyelitis and preserves bone. Bone 2003; 32(4): 397-404.
12. 11- Sherlin DG, Verma R. Vitamin D ameliorates fluoride-induced embryotoxicity in pregnant rats. Neurotoxicology teratology 2001; 23(2): 197-201.
13. 12- Pazhoohan S, Satarian L, Asghari A-A, Salimi M, Kiani S, Mani A-R, et al. Valproic Acid attenuates disease symptoms and increases endogenous myelin repair by recruiting neural stem cells and oligodendrocyte progenitors in experimental autoimmune encephalomyelitis. Neurodegenerative Dis 2013; 13(1): 45-52.
14. 13- Cantorna MT. Vitamin D and autoimmunity: is vitamin D status an environmental factor affecting autoimmune disease prevalence? Proceedings of the society for Experimental Biology Med 2000; 223(3): 230-3.
15. 14- Spach KM, Hayes CE. Vitamin D3 confers protection from autoimmune encephalomyelitis only in female mice. The J Immunol 2005; 175(6): 4119-26.
16. 15- Forster RE, Jurutka PW, Hsieh J-C, Haussler CA, Lowmiller CL, Kaneko I, et al. Vitamin D receptor controls expression of the anti-aging klotho gene in mouse and human renal cells. Biochem Biophys Res Commun 2011; 414(3): 557-62.
17. 16- Yamamoto M, Clark JD, Pastor JV, Gurnani P, Nandi A, Kurosu H, et al. Regulation of oxidative stress by the anti-aging hormone klotho. J Bio Chem 2005; 280(45): 38029-34.
18. 17- Kokkinaki M, Abu-Asab M, Gunawardena N, Ahern G, Javidnia M, Young J, et al. Klotho regulates retinal pigment epithelial functions and protects against oxidative stress. The J Neurosci 2013; 33(41):16346-59.
19. 18- Kuwahara N, Sasaki S, Kobara M, Nakata T, Tatsumi T, Irie H, et al. HMG-CoA reductase inhibition improves anti-aging klotho protein expression and arteriosclerosis in rats with chronic inhibition of nitric oxide synthesis. Int J Cardiol 2008; 123(2): 84-90.
20. 19- Mana P, Linares D, Fordham S, Staykova M, Willenborg D. Deleterious role of IFNγ in a toxic model of central nervous system demyelination. Am J pathol 2006;168(5): 1464-73.
21. 20- Imazeki I, Matsuzaki J, Tsuji K, Nishimura T. Immunomodulating effect of vitamin D3 derivatives on type-1 cellular immunity. Biomed Res 2006; 27(1): 1-9.
22. 21- Lemire J. 1, 25-Dihydroxyvitamin D3–a hormone with immunomodulatory properties. Zeitschrift für Rheumatologie 2000; 59(1): I24-I7.
23. 22- Mattner F, Smiroldo S, Galbiati F, Muller M, Di Lucia P, Poliani PL, et al. Inhibition of Th1 development and treatment of chronic-relapsing experimental allergic encephalomyelitis by a non-hypercalcemic analogue of 1, 25-dihydroxyvitamin D3. Eur J immunol 2000; 30(2): 498-508.
24. 23- Mosayebi G, Ghazavi A, Payani MA. Effect Of Sesame Oil On The Production Of Ifn-Gamma And Il-10 From Th1 And Th2 Cells In C57bl/6 Mice With Experimental Autoimmune Medical journal of Tabriz University of Medical sciences 2007; 29(3).
25. 24- Cantorna Mt. Vitamin D Multiple Sclerosis And Inflammatory Bowel Disease. Archives of Biochemistry Biophysics 2012; 523(1): 103-6.
26. 25- Gill D, Tan PH. Induction of pathogenic cytotoxic T lymphocyte tolerance by dendritic cells: a novel therapeutic target. Expert Opin Ther Targets 2010; 14(8): 797-824.
27. 26- Binyamin O, Larush L, Frid K, Keller G, Friedman-Levi Y, Ovadia H, et al. Treatment of a multiple sclerosis animal model by a novel nanodrop formulation of a natural antioxidant. Int J Nanomedicine 2015; 10: 7165-74.
28. 27- Kesby JP, Eyles DW, Burne TH, McGrath JJ. The effects of vitamin D on brain development and adult brain function. Mol Cell endocrinol 2011; 347(1): 121-7.
29. 28- Chang JM, Kuo MC, Kuo HT, Hwang SJ, Tsai JC, Chen H-C, et al. 1-α, 25-Dihydroxyvitamin D 3 regulates inducible nitric oxide synthase messenger RNA expression and nitric oxide release in macrophage-like RAW 264.7 cells. J Lab Clin Med 2004; 143(1): 14-22.
30. 29-Moreno JA, Izquierdo MC, Sanchez-Niño MD, Suárez-Alvarez B, Lopez-Larrea C, Jakubowski A, et al. The inflammatory cytokines TWEAK and TNFα reduce renal klotho expression through NFκB. J Am Soc Nephrol 2011; 22(7): 1315-25