Volume 25, Issue 7 (oct 2017)                   JSSU 2017, 25(7): 585-594 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

tavalaee M, Arbabian M, Nasr- Esfahani M H. Comparison of sperm PLCζ protein and DNA damage between fertile and infertile men . JSSU 2017; 25 (7) :585-594
URL: http://jssu.ssu.ac.ir/article-1-4096-en.html
Abstract:   (3968 Views)
Introduction: Sperm-specific phospholipase C zeta (PLCζ) protein is expressed during spermatogenesis and plays a main role during oocyte activation. In this study, the expression of sperm PLCζ protein and DNA damage in fertile and infertile men was assessed.
Methods: In this case-control study, semen samples were collected from 15 fertile and 10 infertile men candidated for intra-cytoplasmic sperm injection (ICSI). Sperm parameters, the expression of PLCζ protein, and DNA damage were assessed by the World Health Organization (2010) protocol, Western Blot, and TUNEL assay, respectively.
Results: The quality of sperm parameters were significantly lower in the infertile men compared with the fertile men. In addition, the expression of PLCζ protein was significantly lower, and percentage of sperm DNA damage were significantly higher in infertile men than fertile men. 
Conclusion: Our results clearly showed that low or absence expression of PLCζ and sperm DNA damage could be considered as factors involved in failed fertilization in these infertile men. Therefore, the evaluation of some tests such as PLCζ protein and chromatin tests to assess fertilization potential of a semen sample for infertile men are recommended.
Full-Text [PDF 765 kb]   (1992 Downloads)    
Type of Study: Original article | Subject: Physiology
Received: 2017/02/4 | Accepted: 2017/05/20 | Published: 2017/11/25

1. World Health Organization. WHO laboratory manual for the examination and processing of human semen. Cambridge: Cambridge University Press, 2010.
2. ESHRE, ART fact sheet. [Internet] http://www.eshre.eu/Guidelines-and-Legal/ ART-fact-sheet.aspx 2014, date last accessed.
3. Chandra A, Copen CE, Stephen EH. Infertility service use in the United States: data from the National Survey of Family Growth, 1982-2010. Natl Health Stat Report 2014; 22 (73): 1-21.
4. Allen C, Reardon W. Assisted reproduction technology and defects of genomic imprinting. BJOG 2005; 112(12): 1589-94.
5. Barroso G, Valdespin C, Vega E, Kershenovich R, Avila R, Avendaño C, et al. Developmental sperm contributions: fertilization and beyond. Fertil Steril 2009; 92(3): 835-48.
6. Beck-Fruchter R, Lavee M, Weiss A, Geslevich Y, Shalev E. Rescue intracytoplasmic sperm injection: a systematic review. Fertil Steril 2014; 101(3): 690-8.
7. Swain JE, Pool TB. ART failure: oocyte contributions to unsuccessful fertilization. Hum Reprod Update 2008; 14(5): 431-46.
8. Yanagida K, Fujikura Y, Katayose H. The present status of artificial oocyte activation in assisted reproductive technology. Reprod Med Biol 2008; 7(3): 133–142.
9. Vanden Meerschaut F, Nikiforaki D, Heindryckx B, De Sutter P. Assisted oocyte activation following ICSI fertilization failure. Reprod Biomed Online 2014; 28(5): 560-71.
10. Kashir J, Heindryckx B, Jones C, De Sutter P, Parrington J, Coward K. Oocyte activation, phospholipase C zeta and human infertility. Hum Reprod Update 2010; 16(6): 690-703.
11. Amdani SN, Yeste M, Jones C, Coward K. Sperm Factors and Oocyte Activation: Current Controversies and Considerations. Biol Reprod 2015; 93(2): 50.
12. Amdani SN, Yeste M, Jones C, Coward K. Phospholipase C zeta (PLCζ) and male infertility: Clinical update and topical developments. Adv Biol Regul 2016; 61 :58-67.
13. Nasr-Esfahani MH, Deemeh MR, Tavalaee M. Artificial oocyte activation and intracytoplasmic sperm injection. Fertil Steril 2010; 94(2): 520-6.
14. Aydinuraz B, Dirican EK, Olgan S, Aksunger O, Erturk OK. Artificial oocyte activation after intracytoplasmic morphologically selected sperm injection: A prospective randomized sibling oocyte study. Hum Fertil (Camb) 2016; 19(4): 282-288.
15. Deemeh MR, Tavalaee M, Nasr-Esfahani MH. Health of children born through artificial oocyte activation: a pilot study. Reprod Sci 2015; 22(3): 322-8.
16. Montag M, Köster M, van der Ven K, Bohlen U, van der Ven H. The benefit of artificial oocyte activation is dependent on the fertilization rate in a previous treatment cycle. Reprod Biomed Online 2012; 24(5): 521-6.
17. Tavalaee M, Nasr-Esfahani MH. Expression profile of PLCζ, PAWP, and TR-KIT in association with fertilization potential, embryo development, and pregnancy outcomes in globozoospermic candidates for intra-cytoplasmic sperm injection and artificial oocyte activation. Andrology 2016; 4(5):850-6.
18. Kheirollahi-Kouhestani M, Razavi S, Tavalaee M, Deemeh MR, Mardani M, Moshtaghian J, et al. Selection of sperm based on combined density gradient and Zeta method may improve ICSI outcome. Hum Reprod 2009; 24(10): 2409-16.
19. Li S, Winuthayanon W. Oviduct: roles in fertilization and early embryo development. J Endocrinol 2017; 232(1): R1-R26.
20. Aarabi M, Yu Y, Xu W, Tse MY, Pang SC, Yi YJ, et al. The testicular and epididymal expression profile of PLCζ in mouse and human does not support its role as a sperm-borne oocyte activating factor. PLoS One 2012; 7(3):e33496.
21. Heytens E, Schmitt-John T, Moser JM, Jensen NM, Soleimani R, Young C, et al. Reduced fertilization after ICSI and abnormal phospholipase C zeta presence in spermatozoa from the wobbler mouse. Reprod Biomed Online 2010; 21(6):742-9.
22. Sanusi R, Yu Y, Nomikos M, Lai FA, Swann K. Rescue of failed oocyte activation after ICSI in a mouse model of male factor infertility by recombinant phospholipase Cζ. Mol Hum Reprod 2015; 21(10): 783-91.
23. Kamali-Dolat Abadi M, Tavalaee M, Shahverdi A, Nasr-Esfahani MH. Evaluation of PLCζ and PAWP Expression in Globozoospermic Individuals. Cell J 2016; 18(3):438-45.
24. Aghajanpour S, Ghaedi K, Salamian A, Deemeh MR, Tavalaee M, Moshtaghian J, et al. Quantitative expression of phospholipase C zeta, as an index to assess fertilization potential of a semen sample. Hum Reprod 2011; 26(11): 2950-6.
25. Escoffier J, Yassine S, Lee HC, Martinez G, Delaroche J, Coutton C, et al. Subcellular localization of phospholipase Cζ in human sperm and its absence in DPY19L2-deficient sperm are consistent with its role in oocyte activation. Mol Hum Reprod 2015; 21(2): 157-68.
26. Lambard S, Galeraud-Denis I, Martin G, Levy R, Chocat A, Carreau S. Analysis and significance of mRNA in human ejaculated sperm from normozoospermic donors: relationship to sperm motility and capacitation. Mol Hum Reprod 2004; 10(7): 535-41.
27. Janghorban-Laricheh E, Ghazavi-Khorasgani N, Tavalaee M, Zohrabi D, Abbasi H, Nasr-Esfahani MH. An association between sperm PLCζ levels and varicocele? J Assist Reprod Genet 2016; 33(12): 1649-55.

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | SSU_Journals

Designed & Developed by : Yektaweb