The Effect of Progesterone on Lipid Peroxidation and in Rat  Alzheimer Model by Local Injection of Streptozotocin

Mozafari , M; Hatami , H; Banan , M; Dehgan , G

Volume 24, Issue 7 (Oct 2016) JSSU 2016, 24(7): 587-597 | Back to browse issues page

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Mozafari M, Hatami H, Banan M, Dehgan G. The Effect of Progesterone on Lipid Peroxidation and in Rat Alzheimer Model by Local Injection of Streptozotocin. JSSU 2016; 24 (7) :587-597
URL: http://jssu.ssu.ac.ir/article-1-3563-en.html

The Effect of Progesterone on Lipid Peroxidation and in Rat Alzheimer Model by Local Injection of Streptozotocin

M Mozafari

, H Hatami ^*

, M Banan

, G Dehgan

Abstract: (6019 Views)

Introduction: Alzheimer’s disease is one of the most prevalent brain neurodegenerative diseases and the most common cognitive deficits are memory dysfunction and spatial perception impairment. Progesterone has a neorostroid action in hippocampal neurogenesis, synaptic stability and spatial learning and memory and has antioxidant effect. Since oxidative stress is involved in the pathogenesis of Alzheimer's disease, and conflicting results were reported about the effects of progesterone on therefore in Alzheimer’s, the present study investigated the effect of microinjection of three doses of progesterone on the process of spatial memory and lipid peroxidation and total peroxidation in the hippocampus in the experimental model of AD.

Methods: 42 adult male Wistar rats weighting 250±50 g were randomly divided into six main groups (n=7): control group, sham group, Alzheimer’s model (intracerebroventricular injection of streptozotocin), Alzheimer model + progesterone (0.5, 1.5, 2µg/kg). 2 weeks after injection of streptozotocin (3 mg/kg), Alzheimer's was induced then progesterone injected in different doses. After termination of behavioral testing days, the hippocampus was removed to measure the levels of oxidative stress parameter to measure levels of oxidative stress parameters. Statistical analysis was performed using analysis of variance (ANOVA).

Results: Results showed that intraventricular injection of STZ decreased spatial memory in comparison to control group (P<0.001). Administration progesterone significantly improved spatial memory in experimental models of Alzheimer's rats (P<0.001). MDA and SOD in all doses of progesterone showed a significant reduction compared to the AD animals (P<0.001).

Conclusion: Progesterone can prevent a decrease in spatial learning and memory by reducing oxidative stress in the hippocampus of rats with Alzheimer's.

Keywords: Alzheimer, Spatial Memory, Progesterone, Peroxidation

Full-Text [PDF 595 kb] (1549 Downloads)

Type of Study: Original article | Subject: Physiology
Received: 2016/01/10 | Accepted: 2016/08/6 | Published: 2016/12/26

References

1. 1- Somasundaram R, Dhanaraju MD, Sanjay AS. ALZHEIMER’S DISEASE AND POTENTIAL THERAPEUTIC TARGETS. Pharmacie Globale (IJCP) 2013; 4(5): 1.

2. 2- Elsayad C, Madeo J. The Role of Oxidative Stress in Alzheimer’s disease, Madeo and Elsayad. J Alzheimer’s Dis Parkinsonism 2013; 3: 2.

3. 3- Melcangi RC, Giatti S, Calabrese D, Pesaresi M, Cermenati G, Mitro N, et al. Levels and actions of progesterone and its metabolites in the nervous system during physiological and pathological conditions. Progress in Neurobiology 2014; 113: 56-59.

4. 4- Varadarajan S, Kanski J, Aksenova M, Lauderback C, Butterfield DA. Allan Butterfield. Different Mechanisms of Oxidative Stress and Neurotoxicity for Alzheimer’s Aâ(1-42) and Aâ(25-35). J Am Chem Soc 2001; 123(24): 5625-31.

5. 5- Stein DG, Hoffman SW. Estrogen and progesterone as neuroprotective agents in the treatment of acute brain injuries. Pediatr Rehabil 2003; 6(1): 13-22.

6. 6- Rosario ER, Ramsden M, Pike CJ. Progestins inhibit the neuroprotective effects of estrogen in rat Hippocampus. Brain research 2006; 1099(1): 206-210.

7. 7- Massafra C, Gioia D, De Felice C, Muscettola M, Longini M, Buonocore G. Gender-related differences in erythrocyte glutathione peroxidase activity in healthy subjects. Clin Endocrinol 2002; 57(5): 663-667.

8. 8- Elsayad C, Madeo J. The Role of Oxidative Stress in Alzheimer’s disease. Madeo and Elsayad. J Alzheimer’s Dis Parkinsonism 2013; 3: 2.

9. 9- Martins RN, Harper CG, Stokes GB, Masters CL. Increased cerebral glucose-6-phosphate dehydrogenase activity in Alzheimer’s disease may reflect oxidative stress. J Neurochem 1986; 46(4): 1042-1045.

10. 10- Smith MA, Hirai K, Hsiao K, Pappolla MA, Harris PL, Siedlak SL, et al. Amyloid-_ deposition in Alzheimer transgenic mice is associated with oxidative stress. J Neurochem 1998; 70(5): 2212-2215.

11. 11- Šalković-Petrišić M, Knezović A, Hoyer S, Riederer P. What have we learned from the streptozotocin-induced animal model of sporadic Alzheimer's disease, about the therapeutic strategies in Alzheimer's research? Journal of Neural Transmission 2013; 120(1): 233-52.

12. 12- Markesbery WR, Carney JM. Oxidative alterations in Alzheimer's disease. Brain Pathol 1999; 9(1): 133-146.

13. 13- Mahadik SP, Mukherjee S, Correnti I, Sheffer R. Elevated levels of lipid peroxidation products in plasma from drug-naive patients at onset of psychosis. Schizophr Res 1995; 15: 6670.

14. 14- Kuloghi M, Ustundag B, Atmaca M, Canatan H, Tezean AE, Cinkiline N. Lipid peroxidation and antioxidant enzyme levels in patients with schizophrenia and bipolar disorder. Cell Biochem Funct 2002; 20(2): 171-175.

15. 15- Reddy R, Sahebarao MP, Mukherjee S, Murthy JN. Enzymes of the antioxidant system in chronic schizophrenic patients. Biol Psychiatry 1991; 30(4): 409-12.

16. 16- Sugino N, Karube-Harada A, Kashida S, Takiguchi S, Kato H. Differential regulation of copper- zinc superoxide dismutase and manganese superoxide dismutase by progesterone wihrawal n human endometrial stromal cells. Molecular Human Reproduction 2002; 8(1): 68-74.

17. 17- ŠalkovićPetrišić M, Knezović A, Hoyer S, Riederer P. What have we learned from the streptozotocininduced animal model of sporadic Alzheimer's disease, about the therapeutic strategies in Alzheimer's research. Journal of Neural Transmission 2013; 120(1): 233-52.

18. 18- Markesbery WR, Carney JM. Oxidative alterations in Alzheimer's disease. Brain Pathol 1999; 9(1): 133-146.

20. 19- Mohammadzadeh E, Alipour F, Khallaghi B. Evaluation of Spatial Memory Impairment after Intracerebroventricular Streptozocin Injection in Adult Rats. Shefaye Khatam 2014; 2(1): 40-5.

21. 20- Dolatabadi HRD, Reisi P, Malekabadi HRA, Alaei H, Pilehvarian AA. Effects of Folic Acid on Passive Avoidance Learning and Memory in Rat Alzheimer Model by Intracerebroventricular Injection of Streptozotocin. J Isfahan Med School 2010; 28(112).

22. 21- Iranmanesh F, Sayyadi A, Fayegh A, Shafiee Z. Surveying of estrogen and progesterone effect on electroencephalogram and mini-mental status examination (MMSE) in female patients with Alzheimer’s. Dis Birjand Univ J 2004; 13(2): 9-15.

23. 22- Shahrivar T, Moazedi AA, Rasekh AR, Almasi-Turk S, Roozbehi. A The effects of intrahippocampus injection of progesterone on passive avoidance learning and memory in adult male rats. ISMJ 2014; 17(4): 524-532.

25. 23- Rajabian H, Mirzazadeh E, Jand Y, Rad G. Effect of progesterone administration in newborns rats on Morris Water Maze learning susceptibility after adolescence. J Arak Univ Med Sci 2009; 11(4): 23-31.

26. 24- Orr PT, Lewis MC, Frick KM. Dorsal hippocampal progesterone infusions enhance object recognition in young female mice. Pharmacology, Biochemistry and Behavior 2009; 93(2): 177-182.

27. 25- Frye CA, Lacey EH. Progestins in"uence performance on cognitive tasks independent of changes in affective behavior. Psychobiology 2000; 28(4): 500-63.

28. 26- Walf AA, Rhodes ME, Frye CA. Ovarian steroids enhance object recognition in naturally cycling and ovariectomized, hormone-primed rats. Neurobiol Learn Mem 2006; 86(1): 35-64.

29. 27- Ferdowsi K, Hatami H, Dehghan G. Evaluation the Simultaneous Effects of Progesterone and Vitamin C on Anxiety in Male Wistar Rats. Armaghane-danesh, Yasuj Univ Med Sci J(YUMSJ) 2015; 20(6): 516-525.

30. 28- Unfer TC, Maurer LH , Kemerich DM, Figueiredo CG, Duarte MMF, Gelain DP, et al. N on-genomic, direct modulatory effect of 17b-estradiol, progesterone and their synthetic derivatives on the activity of human erythrocyte CuZn superoxide dismutase. Free Radic Res 2013; 47(3): 219-32.