Volume 24, Issue 2 (May 2016)                   JSSU 2016, 24(2): 174-184 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Gharirvand Eskandari E, Doudi M. Investigation of Antileishmanial Effect of Alcoholic Extract and Essential Oil of Medicinal Plant Leaf Black Alfalfa (Medicago Lupulina), on The Number of Clinical Isolates of Leishmania Major Promastigotes in Vitro . JSSU. 2016; 24 (2) :174-184
URL: http://jssu.ssu.ac.ir/article-1-3557-en.html
Abstract:   (5325 Views)

Introduction: Leishmaniasis has created enormous global health problems. Side effects, drug resistance and the lack of effective vaccines had led to the new effective compounds effective of plants. The aim of this study was to introduce a traditional medicinal plant called Black alfalfa (Medicago Lupulina) that can be used as a valuable resource against cutaneous leishmaniasis.

Methods: In this experimental study, alcoholic extract was prepared by maceration and essential oil by distillation water method. Leishmania major promastigotes were cultured at 25 ± 2° C in N.N.N culturemedium, then in Schneider and next were cultured in RPMI- 1640. afterward, using MTT (Methyl Thiazole Tetrazolium), the IC50 (Inhibitory Concentrations 50%) for extracts, essence and Glucantime were determined. The results using Tukey and t-test were analyzed and were presented by software SPSS16. MTT assay were repeated. 3 times for each sample.

Results: IC50 for alfalfa leaf extract and essential oil of black alfalfa and Glucantime against L. major promastigotes was determined after 24, 48 and 72 hours, 240, 130 and 69 micrograms per ml, and 801, 340 and 190 micrograms per ml, also 26, 19 and 11 micrograms per ml , respectively. There was a significant differences between the IC50 plant extract and essential oil and Glucantime after 24, 48 and 72 hours.

Conclusion: Alcoholic extracts and essential oil the plant had significant anti leishmaniasis effects in vitro. In this way, it can be considered as an anti-leishmaniasis among the herbs.

Full-Text [PDF 517 kb]   (1894 Downloads)    
Type of Study: Original article | Subject: Microbiology
Received: 2016/01/8 | Accepted: 2016/04/9 | Published: 2016/07/19

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


© 2021 All Rights Reserved | SSU_Journals

Designed & Developed by : Yektaweb