Volume 24, Issue 6 (sep 2016)                   JSSU 2016, 24(6): 479-490 | Back to browse issues page

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Abstract:   (5460 Views)

Introduction: Saffron has some applications such as anti-spasmodic and analgesic effects in traditional medicine. The aim of this study was to evaluate the anti melanogenesis and antioxidant properties of crocin (one of the saffron active components) on B16F10 mouse melanoma cell line.

Methods: In this experimental study, B16F10 cells were prepared from Pasteur Institute (NCBI) and were grown in medium containing 10% FBS with 1% antibiotics at 37° C.Then, the cell viability was assessed, the inhibitory effect of crocin on melanogenesis was investigated by fungal tyrosinase,and  tyrosinase activity assessment, melanin synthesis and cellular antioxidant capacity was evaluated after treatment with various concentrations of crocin (1, 2, 4, 8, 16 µg/ ml) by DCFDA assay. Quantitative data were analyzed using SPSS software, one way ANOVA, Tukey test at p ≤0.05.

Results: The data revealed no significant cytotoxicity of crocin(p>0.05) (concentrations of 1-16 µg/ ml)
on B16F10 cells, in addition, fungal tyrosinase activity (***p≤ 0.001), intracellular tyrosinase activity
(*p< 0.05, **p≤ 0.01) and melanin content (*p< 0.05, **p≤ 0. 01 , ***p≤ 0.001)  have suppressed in a dose-dependent manner. Further, reduction of free radicals in treated melanoma cells exhibited that crocin in concentrations of 4and 8 µg/ ml in p<0.05 and-16 µg/ mlin p≤ 0.001 possess significant antioxidant efficacy.

Conclusion: Our results elucidated that crocin was proposed as melanogenesis inhibitor in B16F10 cells, which can be considered as an effective whitening agent.

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Type of Study: Original article | Subject: Biology
Received: 2015/06/19 | Accepted: 2015/10/19 | Published: 2016/10/9

1. 1- Cha J, Kim S. Anti-melanogenesis in B16F0 Melanoma Cells by Extract of Fermented Cordyceps militaris Containing High Cordycepin. J Life Sci 2013; 23(12): 1516-24.
2. 2- Wu L, Chang L, Chen S, Fan N, Ho JA. Antioxidant activity and melanogenesis inhibitory effect of the acetonic extract of Osmanthus fragrans: A potential natural and functional food flavor additive. Food Sci Technol 2009; 42(9):b1513-9.
3. 3- Sariri R, Sabbaghzadeh R, Poumohamad F. In-Vitro Antioxidant and Anti-Tyrosinase Activity of Methanol Extracts from Crocus Sativus Flowers. Pharmacology Online 2011; 3: 1-11.
4. 4- Moreira CG, Horinouchi CDS, Souza-filho CS, Campos FR, Barison A, Cabrini DA, et al. Hyperpigmentant activity of leaves and flowers extracts of Pyrostegia venusta on murine B16F10 melanoma. J Ethnopharmacol 2012; 141(3): 1005-11.
5. 5- Yoon WJ, Ham YM, Yoon HS, Lee WJ, Lee NH, Hyun CG. Acanthoic Acid Inhibits Melanogenesis through Tyrosinase Down- regulation and Melanogenic Gene Expression in B16 Melanoma Cells. Nat Prod Commun 2013; 8: 1-4.
6. 6- Zhou J, Shang J, Ping F, Zhao G. Alcohol extract from Vernonia anthelmintica ( L .) willd seed enhances melanin synthesis through activation of the p38 MAPK signaling pathway in B16F10 cells and primary melanocytes. J Ethnopharmacol 2012; 143(2): 639-47.
7. 7- Tsai T, Huang C, Wu W, Huang W, Chyuan J, Tsai P. Antioxidant, cell-protective, and anti-melanogenic activities of leaf extracts from wild bitter melon (Momordica charantia Linn. var. abbreviata Ser.) cultivars. Bot Stud 2014; 55(78): 1-11.
8. 8- Boonpisuttinant K, Ruksiriwanich W, Winitchai S. In Vitro Anti-melanogenesis and Collagen Biosynthesis Stimulating Activities of Star Grass (Hypoxis aurea Lour) Extracts. Asian J Appl Sci 2014; 2(4): 405-13.
9. 9- Huang H, Hsieh W, Niu Y, Chang T. Inhibition of melanogenesis and antioxidant properties of Magnolia grandiflora L. flower extract. Complement Altern Med 2012; 12(1): 1-9.
10. 10- Ebanks JP, Wickett RR, Boissy RE. Mechanisms Regulating Skin Pigmentation: The Rise and Fall of Complexion Coloration. Int J Mol Sci 2009; 10: 4066-87.
11. 11- Smit N, Vicanova J, Pavel S. The Hunt for Natural Skin Whitening Agents. Int J Mol Sci 2009; 10: 5326-49
12. 12- Chang T. Natural Melanogenesis Inhibitors Acting Through the Down-Regulation of Tyrosinase Activity. Materials 2012; 5: 1661-85.
13. 13- Han JH, Byeon S, Hyun C, Lee NH. Melanogenesis Inhibitory Activity in the Extracts of Oreocnide fruticosa (Gaudich.) Hand.-Mazz Branches. J Appl Pharm Sci 2014;4(1):166-9.
14. 14- Kuo PC, Damu AG, Cherng CY, Jeng JF, Teng CM, Lee EJ, et al. Isolation of a natural antioxidant, dehydrozingerone from zingiber officinale and synthesis of its analogues for recognition of effective antioxidant and antityrosinase agents. Arch Pharm Res 2005; 28: 518-28.
15. 15- Elmore AR. Final report of the safety assessment of l-ascorbic acid, calcium ascorbate, magnesium ascorbate, magnesium ascorbyl phosphate, sodium ascorbate, and sodium ascorbyl phosphate as used in cosmetics. Int J Toxicol 2005; 24: 51-111.
16. 16- Yao C, Lin Y, Shaaban M, Chen J. Inhibitory effect of ectoine on melanogenesis in B16-F0 and A2058 melanoma cell lines. Biochem Eng J 2013; 78: 163-69.
17. 17- Lee SJ, Lee WJ, Chang SE, Lee G. Antimelanogenic effect of ginsenoside Rg3 through extracellular
18. signal-regulated kinase-mediated inhibition of microphthalmiaassociated transcription factor. J Ginseng Res 2015; 39: 238-42.
19. 18- Hashemi F, Zarei MA. Tyrosinase Inhibitory Activity Within Hexane Extract of Ten Screened Plants From Kurdistan Province of Iran. Int J Adv Biol Biomed Res 2014; 2(11): 2795-99.
20. 19- Hadizadeh F, Mohajeri SA, Seifi M. Extraction and Purification of Crocin from Saffron Stigmas Employing a Simple and Efficient Crystallization Method. Pak J Biol Sci 2010; 13 (14): 691-98.
21. 20- Christodoulou E, Kadoglou NP, Kostomitsopoulos N, Valsami G. Saffron: a natural product with potential pharmaceutical applications. J Pharm Pharmacol 2015;67(12):1634-49.
22. 21- Shirali S, Zahra Bathaie S, Nakhjavani M. Effect of crocin on the insulin resistance and lipid profile of streptozotocin-induced diabetic rats. Phytother Res 2013;27(7):1042-47.
23. 22- Srivastava R, Ahmed H, Dharamveer RK, Saraf SA. Crocus sativus L: A comprehensive review. Pharmacogn Rev 2010; 4(8): 200-08.
24. 23- Gutheil WiG, Reed G, Ray A, Dhard A. Crocetin: an agent derived from saffron for prevention and therapy for cancer. Curr Pharm Biotechnol 2012; 13(1): 173-79.
25. 24- Piperaceae CDC. Antioxidant and Anti-tyrosinase Activities from Piper officinarum C.DC (Piperaceae). J Appl Pharm Sci 2014; 4(5): 87-91.
26. 25- Lee D, Cha B, Lee Y, Kim G, Noh H. The Potential of Minor Ginsenosides Isolated from the Leaves of Panax ginseng as Inhibitors of Melanogenesis. Int J Mol Sci 2015; 16: 1677-90.
27. 26- Qiao Z, Koizumi Y, Zhang M, Natsui M, Flores MJ, Gao L, et al. Anti-Melanogenesis Effect of Glechoma hederacea L. Extract on B16 Murine Melanoma Cells. Biosci J 2012; 10: 1877- 83.
28. 27- Wang TJ, An J, Chen XH, Deng QD, Yang L. Assessment of Cuscuta chinensis seeds׳ effect on melanogenesis: comparison of water and ethanol fractions in vitro and in vivo. J Ethnopharmacol 2014; 154(1): 240- 48.
30. 28- Chiang HM, Chien YC, Wu CH, Kuo YH, Wu WC, Pan YY, et al. Hydroalcoholic extract of Rhodiola rosea L. (Crassulaceae) and its hydrolysate inhibit melanogenesis in B16F0 cells by regulating the CREB/MITF/tyrosinase pathway. Food Chem Toxicol 2014; 65: 129-39.
31. 29- Akhtar N, Muhammad H, Khan S, Ashraf S, Shair I, Ali A. Skin Depigmentation Activity of Crocus sativus Extract Cream. Trop J Pharm Res 2014; 13(11): 1803-08.
32. 30- Kim YJ, Kang KS,Yokozawa T. The anti-melanogenic effect of pycnogenol by its anti-oxidative actions. Food Chem Toxicol 2008; 46(7): 2466-71.
33. 31- Kim YC, Choi SY, Park EY. Anti-melanogenic effects of black, green, and white tea extracts on immortalized melanocytes. Vet Sci 2015; 16(2): 135-43.

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