fa بررسی فراوانی سلول‌های تک هسته‌ای بیان کننده پروتئین MR1 در مایع مفصلی بیماران مبتلا به آرتریت روماتوئید ایمونولوژی Immunology پژوهشي Original article <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b> <span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">آرتریت روماتوئید (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RA</span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">) یک بیماری مفصلی خودایمن التهابی با شیوع 0/5 تا 1 درصدی در جوامع است. جمعیتی از سلول‌های ایمنی به‌نام سلول‌های </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">T</span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> نامتغیر مخاطی (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MAIT</span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">) در انسان شناسایی شده‌اند که هنوز نقش آنها در </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RA</span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> مشخص نشده است. این سلول‌ها به آنتی‌ژن‌های عرضه شده توسط مولکول‌های شبه </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MHC</span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> کلاس یک به نام </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MR1</span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> پاسخ می‌دهند. در این مطالعه میزان بیان مولکول‌های </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MR1</span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> بر سطح سلول‌های تک هسته‌ای در مایع مفصلی بیماران </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RA</span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> بررسی می‌گردد</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">.</span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></b><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> در این مطالعه مورد شاهدی 14 فرد بیمار مبتلا به آرتریت روماتوئید پیشرفته و 14 فرد سالم انتخاب شدند. با کسب رضایت از هر بیمار مایع مفصلی و خون محیطی دریافت شد. از افراد سالم نیز 5 میلی لیتر خون محیطی گرفته شد. فراوانی سلول‌های بیان کننده </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MR1</span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> در مایع مفصلی و خون محیطی با روش فلوسایتومتری سنجیده شد. از آزمون </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Mann&ndash;Whitney</span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> برای مقایسه میانگین درصد سلول‌ها بیان کننده </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MR1</span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> در گروه</span></span><span lang="AR-SA" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">&not;</span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">های مورد مطالعه استفاده شد و </span></span><span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">0/05&nbsp;</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P</span></span><span lang="FA" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">˂</span></span>&nbsp; <span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">معنی</span></span><span lang="AR-SA" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">&not;</span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">دار درنظر گرفته شد.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> به‌طور میانگین 12/5 درصد از سلول‌های مایع مفصلی در بیماران </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RA</span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">، پروتئین </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MR1</span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> را بیان کردند. هم‌چنین 0/65 درصد از سلول‌های خون محیطی این بیماران نیز از نظر </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MR1</span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> مثبت بودند. در گروه کنترل، 0/61 درصد از سلول‌های تک‌هسته‌ای خون محیطی </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MR1</span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> را بیان کردند. فراوانی سلول‌های بیانکننده‌ </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MR1</span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> در خون محیطی افراد سالم و بیماران </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RA</span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> تفاوت معنی‌داری نشان ندادند در حالی‌که فراوانی سلول‌های تک هسته‌ای بیان کننده‌ </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">MR1</span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"> در مایع مفصلی در این بیماران به‌طور معنی‌داری از خون این بیماران و از خون افراد سالم بیشتر بود </span></span><span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">(</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P<0.001</span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">). </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.1pt">نتیجه‌گیری:</span></span></span></b><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.1pt"> نتایج این مطالعه نشان داد که 12/5 درصد از سول‌های تک هسته‌ای مایع مفصلی بیماران </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">RA</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.1pt"> پروتئین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">MR1</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.1pt"> را بیان می‌کنند که می‌تواند به فعالیت سلول‌های </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">MAIT</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.1pt"> منجر شود.</span></span></span></span></span></span></span> <span style="font-size:11pt"><span calibri="" style="font-family:"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Introduction:</span></span></b> <span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disorder with a prevalence of 0.5 to 1 percent in the population. Recently, a population of immune cells known as mucosal-associated invariant T cells (MAIT cells) has been identified in humans, although their role in RA remains unclear. These cells respond to antigens presented by MHC class I-like molecules called MR1. This study examined the expression level of MR1 molecules on the surface of mononuclear cells in the synovial fluid of patients with RA.</span></span><span style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> <span style="font-size:11pt"><span calibri="" style="font-family:"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Methods:</span></span></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> In this case-control study, 14 patients with advanced RA and 14 healthy individuals were selected. Synovial fluid and peripheral blood samples were collected from the patients. Additionally, only blood samples were collected from the healthy individuals. The frequency of MR1-expressing cells in synovial fluid and peripheral blood was measured using flow cytometry and reported as the average percentage of MR1-positive cells.</span></span><span style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> <span style="font-size:11pt"><span calibri="" style="font-family:"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></b><b> </b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">On average, 12.5% of synovial fluid cells from patients with rheumatoid arthritis expressed the MR1 protein. In addition, 0.65% of peripheral blood cells in these patients were positive for MR1. In the control group, 0.61% of peripheral blood mononuclear cells showed MR1 expression. Statistical analysis showed that the expression level of MR1 on mononuclear cells in synovial fluid of rheumatoid arthritis patients was significantly higher than in the blood of these patients and in the blood of healthy individuals (P<0.001).</span></span><span style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> <span style="font-size:11pt"><span calibri="" style="font-family:"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></b><b> </b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">The increased expression of MR1 on the surface of mononuclear cells in the synovial fluid of RA patients may lead to the activation of MAIT cells</span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">.</span></span></span></span> آرتریت روماتوئید, سلول‌های MAIT, سلول‌های تک هسته‌ای مایع مفصلی, MR1 Rheumatoid arthritis, Mucosal-Associated Invariant T, MHC Class I-related protein 1 (MR1). 10177 10185 http://jssu.ssu.ac.ir/browse.php?a_code=A-10-1115-4&slc_lang=fa&sid=1 Vahid Zare وحید زارع vahidzare90imeno@gmail.com 0009-0007-7425-6307 No Department of Immunology, Medicine Faculty, Shahid Sadoughi University of Medical Sciences, Yazd, Iran دانشگاه علوم پزشکی شهید صدوقی ali shams علی شمس alis743@yahoo.com 0000-0002-3914-4568 Yes Shahid Sadoughi University of Medical Sciences دانشگاه علوم پزشکی شهید صدوقی یزد Mohammad Taher tahoori محمدطاهر طهوری immuno.2006@yahoo.com 0000-0003-2304-536X No Shahid Sadoughi University of Medical Sciences دانشگاه علوم پزشکی شهید صدوقی یزد ALI dehghan علی دهقان drdehghanali@yahoo.com 0000-0001-9291-6419 No Shahid Sadoughi University of Medical Sciences دانشگاه علوم پزشکی شهید صدوقی یزد