Volume 26, Issue 3 (Jun 2018)                   JSSU 2018, 26(3): 275-285 | Back to browse issues page

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Barzegari firouzabadi F, Oryan S, Sheikhha M H, Kalantar S M, javed A. Laboratory study: evaluation and comparison of physicochemical properties, level of surface charge and cytotoxicity of lipid nanocomposites for carrying nucleic acid in bone cancer. JSSU. 2018; 26 (3) :275-285
URL: http://jssu.ssu.ac.ir/article-1-4447-en.html
Abstract:   (1477 Views)
Introdution: The aim of this study was to investigate the physico-chemical properties, surface charge and cytotoxicity of nanocomposite formulations for the design of lipid nano-systems with maximum loading for nucleic acids in bone marrow cancer.
Methods For this study, six types of liposomes with different compositions were synthesized using DPPC, cholesterol, DSPE-mPEG (2000) and DOTAP. Then, nanosystems were evaluated for particle size, surface charge, cytotoxicity and loading rate of miRNA in two MG-63 and SaOs2 cell lines, which are human cell bone marrow cell lines.
Results: All nano-systems were monodisperse. Among the systems, the F6 formula has the lowest toxicity and the highest load of miRNA-143, due to the presence of a suitable amount of DOTAP and PEG phospholipid in the liposome structure.
Conclusion: Liposomal formulation with a suitable percentage of DOTAP cationic phospholipid can be used as a successful carrier for the transport of miRNA in the gene therapy process for the treatment of various cancers, especially metastatic types.
Full-Text [PDF 1508 kb]   (474 Downloads)    
Type of Study: Original article | Subject: Biology
Received: 2018/01/13 | Accepted: 2018/02/10 | Published: 2018/08/19

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