Volume 26, Issue 5 (Agu 2018)                   JSSU 2018, 26(5): 426-438 | Back to browse issues page

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malek shahi M, razavian S M H, Aghaee S S. Evaluation of malone-di-aldehide as an indicatorfor semen bacterial contamination . JSSU. 2018; 26 (5) :426-438
URL: http://jssu.ssu.ac.ir/article-1-4442-en.html
Abstract:   (1521 Views)
Introdution: The statistics of infertility among couples is about 15%. 60% of infertility cases are related to male factors, and the share of infertility due to genital tract infection is 20%. In bacteriospermia, the presence of bacteria and leukocytes produce active forms of oxygen and damage to sperm cells. In the present study, determination of frequency and diversity of bacteria involved in urinary tract infection, determination of malondialdehyde in sperm and seminal plasma as an index of oxidative damage and its relation with microbial contamination and effect of oxidative stress and microbial contamination on the quality of semen was evaluated in infertile men.
Methods: This trans-sectional study performed on 53 patients referred to the Infertility Treatment Center of Jahad Daneshgahi. After sampling, semen parameters according to WHO (1999) protocol and bacterial contamination by culture and differential tests and also malondialdehyde concentration in sperms and seminal plasma via fluorimetric method were determined and results analyzed by ANOVA and T test in SPSS16 software.
Results: Based on the results, 92% of the patients had bacterial contamination, including Staphylococcus aureus (65%), Staphylococcus epidermidis (24.5%), Proteus mirabilis (2.4%), Klebsiella (4.8%) and Enterococcus (4.8%). Bacteriospermia had not only a significant effect on the quality of semen, especially sperm motility (p<0.01), but significantly increased (p<0.01) the malondialdehyde concentration in patientschr('39') cells and seminal plasma.
Conclusion: Infection affects semen quality and decreases the chance of a successful ART. Malondialdehyde can be used as a quick and accurate indicator for evaluating bacterial contamination in infertile men.
Full-Text [PDF 1081 kb]   (383 Downloads)    
Type of Study: Original article | Subject: Biochemistry
Received: 2018/01/8 | Accepted: 2018/02/10 | Published: 2019/04/8

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